[1]杨谷良,谭晓风.热硼酸盐法提取梨雌蕊RNA方法改进及提取效果[J].西北林学院学报,2006,21(02):54.
 YANG Guliang,TAN Xiaofeng.Study on Using Hotborate Method in Extracting the Total RNA from the Pistil of Pyrus pyrifolia[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2006,21(02):54.
点击复制

热硼酸盐法提取梨雌蕊RNA方法改进及提取效果()
分享到:

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第21卷
期数:
2006年02期
页码:
54
栏目:
林业基础科学
出版日期:
2006-04-30

文章信息/Info

Title:
Study on Using Hotborate Method in Extracting the Total RNA from the Pistil of Pyrus pyrifolia
作者:
杨谷良1谭晓风2
1.黄冈师范学院 生物系,湖北 黄冈 438000;2. 中南林学院 经济林育种与栽培国家林业局重点实验室,湖南 长沙 410006
Author(s):
YANG Guliang1TAN Xiaofeng2
1.Biology Academy, Huanggang Normal College, Huanggang, Hubei 438000, China;2.Central South Forestry University, Changsha, Hunan 410006, China
关键词:
热硼酸盐法RNA提取雌蕊
Keywords:
hotborate method RNA extraction pear pistil
分类号:
S718.43
文献标志码:
A
摘要:
提取梨雌蕊的总RNA;方法:修改后的热硼酸盐法。采用电泳检测RNA的完整性、并测定了提取物的浓度和纯度、根据 S基因设计引物对提取的总RNA进行3'RACE实验等方法对提取产物进行检测。结果:修改后的整个提取过程可以在1 d内完成,提取的总RNA电泳条带清晰,总RNA提取液的浓度为1.925 μg/L, OD 260/OD 280 值为1.926,3'RACE实验得到了预期的条带。结论:经过改进后的热硼酸盐法适合提取梨雌蕊的RNA。 第2期杨谷良等热硼酸盐法提取梨雌蕊RNA方法改进及提取效果
Abstract:
Objective: To extract the total RNA from the pistil of Pyrus pyrifolia ; an improved hotborate method was used. The integration of the RNA was ascertained by gel electrophoresis; the purity and the concentration was measured, and the test of 3`RACE was completed after the primer of P1 was designed based on the S gene.The results showed that the whole extraction process could be completed in 1 day. The strings of the gel electrophoresis of total RNA were distinctly. The concentration of the extracted RNA liquor was 1.925 μg/L. The value of OD 260/ OD 280 was 1.926 and the expected gel electrophoresis strings from 3`RACE emerged. It is suggested the improved hotborate method is suitable to extract the total RNA from the pistil of P. pyrifolia.

参考文献/References:

[1]陈森,孙坚.国有林场存在的问题及深化改革的思路[J].云南林业调查规划设计,1999, 24(4):3740.
[2]王岩峰,杨永侠.基于GIS牧草种植管理专家系统的分析与设计[J].草业科学,2004, 21(1):6164.
[3]赵鹏祥,李秀信,李卫忠.基于MapGIS的空间分析在林业中的应用[J].西北林学院学报,2002, 17(1):9193. 
[4]钟耳顺.地理信息系统应用与社会背景分析[J].地理研究,1995, 14(2):9197.
[5]宋关福,钟耳顺.组件式地理信息系统研究与开发[J].中国图象图形学报,1998, 3(4):313317.
[6]宋关福,钟耳顺,刘纪远,等.多源空间数据无缝集成研究[J].地理科学进展,2000, 19(2):110115.
[7]Rizzoli A E, Davis J R, Abel D J. Model and data integration and reuse in environmental decision support system[J]. Decision Support System, 1998, 24(2):127144.
[8]李德仁.论RS、GPS与GIS集成的定义、理论与关键技术[J].遥感学报,1997, 1(1):1821.
[9]洪玲霞,唐守正,杜纪山,等.天然林区森林资源数据和图面更新方法[J].林业科学,2001, 37(1):8389.[LM]

相似文献/References:

[1]陈欣,祝传书,冯俊涛*,等. 雷公藤RNA提取方法选择与优化[J].西北林学院学报,2014,29(02):112.[doi:doi:10.3969/j.issn.1001-7461.2014.02.20]
 CHEN Xin,ZHU Chuan-shu,FENG Jun-tao*,et al. The Establishment and Optimization of RNA Isolation in Tripterygium wilfordii [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(02):112.[doi:doi:10.3969/j.issn.1001-7461.2014.02.20]

备注/Memo

备注/Memo:
20050823修回日期:20050916基金项目:湖南省科技三项基金“湖南省主要沙梨品种自交不亲和基因的研究”(0JZY2155)作者简介:杨谷良(1974),男,湖南宁乡人,博士,研究方向为林业生物技术。
更新日期/Last Update: 2010-05-04