[1]王培军涂作菊,周自云,胡景江.假大白菇深层发酵过程中胞外酶活性的变化假大白菇深层发酵过程中胞外酶活性的变化[J].西北林学院学报,2007,22(01):26-30.
WANG Peijun,TU Zuoju,ZHOU Ziyun,et al.Changes of Extracellular Enzyme Activity During the Submerged Culture of Russula pseudodelica Lange[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(01):26-30.
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假大白菇深层发酵过程中胞外酶活性的变化假大白菇深层发酵过程中胞外酶活性的变化()
《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]
- 卷:
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第22卷
- 期数:
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2007年01期
- 页码:
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26-30
- 栏目:
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森林生物学研究
- 出版日期:
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2007-01-30
文章信息/Info
- Title:
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Changes of Extracellular Enzyme Activity During the Submerged Culture of Russula pseudodelica Lange
- 作者:
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王培军1; 3涂作菊2; 周自云3; 胡景江3
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1.太原市园林科研所,山西 太原 030012;2. 陕西省紫阳县植保站 725300;3. 西北农林科技大学 生命科学学院,陕西 杨陵 712100
- Author(s):
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WANG Peijun1; TU Zuoju2; ZHOU Ziyun3; HU Jingjiang3
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1.Landscape Gardening Institute of Taiyuan, Taiyuan, Shanxi 030012,China; 2. Plant Protecting Station of Ziyang, Shaanxi 725300, China; 3.College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China
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- 关键词:
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假大白菇; 深层发酵; 胞外蛋白质; 胞外还原糖; 淀粉酶; 纤维素酶
- Keywords:
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Russula pseudodelica Lange; submerged culture; extracellular protein; extracellular deoxioized saccharide; cellulase
- 分类号:
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S718.81
- 文献标志码:
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A
- 摘要:
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本文首次对假大白菇深层发酵过程中的5项生理指标与6种胞外酶的活性进行了测定,结果表明,菌丝生物量在第10 d达到最大值;pH值先降后升;胞外还原糖与蛋白质浓度分别于第5 d、第6 d达到峰值;胞外多糖含量在第6 d出现峰值;淀粉酶、CMC酶、纤维素脱脂棉酶与滤纸酶的酶活变化趋势相近;愈创木酚氧化酶与邻苯二酚氧化酶的酶活性很小。
- Abstract:
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Dynamic variations of 5 physiological indices and octivities of 6 kinds of extracellular enzymes during the submerged culture of Russula pseudodelica Lange. were investigated. The results showed that the maximum of hypha growth was 6.58 g·L-1,appeared in the 10th day; pH of fluid medium decreased, then increased; the peak times of extracellular protein and deoxioized saccharide were in the 5th day, the 6th day; extracellular polysaccharide appeared its maximum in the 6th day ; dynamic variation of diastase, CMC enzyme, ellulose during the submerged culture were quite similar; the activities of guaiacol oxidase and polyphenol oxidase were very low during the submerged culture.
参考文献/References:
[1]卯晓岚.中国经济真菌[M].北京科学出版社,1998.503 .[2]卯晓岚.中国大型真菌[M].河南河南科学技术出版社,2000.[3]高俊凤.植物生理学实验技术[M].西安世界图书出版西安公司,2000.137138 .[4]陈毓荃.生物化学实验方法和技术[M].北京.科学出版社.2002.[5]倪新江,潘迎捷.袋载香菇生长期间不同料层中木质纤维素的降解及有关酶活性的变化[J].食用菌学报.1998,5(1)1317.[6]中国科学院上海植物生理研究所.现代植物生理学实验指南[M].北京科学出版社.1999.124.[7]王玉万,徐文玉.构菌栽培过程中对木质纤维素的降解和几种多糖分解酶活性的变化[J].微生物学通报.1989,16(3)137140.[8]王宜磊,邓振坦,赵良田. 彩绒革盖菌愈创木酚氧化酶活性研究[J].植物学通报,2000,17(5);462465.[9]刘祖同,罗信昌.食用蕈菌生物技术及应用[M].北京.清华大学出版社,2002.(上接第14页)3小结与讨论柠条叶片在阳光充足的夏季晴天,其Pn日进程是一条单峰曲线,Pn在1100时左右达到最大值,约为8.092μmol·m-2·s-1;柠条TRAN的日变化也为一单峰曲线,但柠条TRAN日变化峰值出现在1300
备注/Memo
- 备注/Memo:
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收稿日期20060410修回日期20060429基金项目教育部优秀博士论文基金(200057)作者简介王培军 (1979),男,山西盂县人,硕士,研究方向为生物工程。
更新日期/Last Update:
2010-05-04