[1]刘兴菊,梁海永,杨敏生*,等.发根农杆菌rolC基因的克隆及序列分析[J].西北林学院学报,2007,22(02):72-75.
　LIU Xing-ju,LIANG Hai-yong,YANG Min-sheng*,et al.Cloning of rolC Gene of Ri Plasmid and Sequence Analysis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(02):72-75.
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发根农杆菌rolC基因的克隆及序列分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第22卷
期数:
2007年02期
页码:
72-75
栏目:
林木遗传育种与栽培
出版日期:
2007-03-30

文章信息/Info

Title:
Cloning of rolC Gene of Ri Plasmid and Sequence Analysis
文章编号:
1001-7461(2007)02-0072-04
作者:
刘兴菊; 梁海永;  杨敏生*;  周怀军
(河北农业大学 林学院，河北 保定 071000)
Author(s):
LIU Xing-ju; LIANG Hai-yong; YANG Min-sheng*; ZHOU Huai-jun
（College of Forestry; Agricultural University of Hebei; Baoding; Hebei 071000; China）
关键词:
Ri质粒;  rolC基因;  克隆
Keywords:
Ri plasmid;  rolC gene;  sequence analysis
分类号:
S718.46
文献标志码:
A
摘要:
以发根农杆菌30148为材料,设计了rolC基因的特异性引物,应用PCR技术,扩增出了正确序列的rolC基因585 bp片段,并利用pUCm-T载体对此片段进行了克隆,并进行了序列分析。所克隆rolC基因与已发表的序列相比较,核苷酸序列的同源性达到100%,但另一个克隆中部分碱基发生了改变,同源性达到99%。
Abstract:
Ri plasmid was developed and a 585bp fragment of rolC gene was amplified by PCR via primer of rolC gene,and the fragment was cloned into vector pUCm-T.Comparison with previously published nucleotide sequence showed that the homology was 100%.But another clone gene nucleotide sequence showed that the homology was 99% with it.

参考文献/References:

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［7］ 萨姆布鲁克J,弗里奇EF,曼尼阿蒂斯T著.金冬雁,黎孟枫译.分子克隆实验指南［M］.北京:科学出版社,1999.
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备注/Memo

备注/Memo:
收稿日期：2006-04-27 修回日期：2006-06-27
基金项目：河北省自然科学基金（302312)
作者简介：刘兴菊（1976-) ，女,河北涿州人，硕士，从事林业生物技术及林木育种研究.
*通讯作者：杨敏生（1962-) ，男，内蒙古多伦人，教授，博士生导师，从事林木遗传育种工作.
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