[1]苏丽媛,周索,杨建伟,等.异三聚体G蛋白在2,4D诱导的拟南芥根生长发育中的作用[J].西北林学院学报,2007,22(03):92-95.
 SU Liyuan,ZHOU Suo,YANG Jianwei,et al.The Role of Heterotrimeric G Protein in 2,4D Induction , Growth and Development of Arabidopsis thaliana Roots[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(03):92-95.
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异三聚体G蛋白在2,4D诱导的拟南芥根生长发育中的作用()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第22卷
期数:
2007年03期
页码:
92-95
栏目:
林木繁育与栽培
出版日期:
2007-06-30

文章信息/Info

Title:
The Role of Heterotrimeric G Protein in 2,4D Induction , Growth and Development of Arabidopsis thaliana Roots
作者:
苏丽媛1周索2杨建伟2梁宗锁1*
1.西北农林科技大学,陕西 杨陵 712100;2.南阳师范学院 生物系,河南 南阳 473061
Author(s):
SU Liyuan1ZHOU Suo2YANG Jianwei2LIANG Zongsuo1
1.Northwest A F University; Yangling, Shaanxi 712100,China;2.Department of Biology, Nanyang Teachers College, Nanyang, Henan 473061,China
关键词:
拟南芥生长素异三聚体G蛋白生长发育
Keywords:
Arabidopsis thaliana auxin heterotrimeric G protein growth and development
分类号:
Q946.1
文献标志码:
A
摘要:
利用拟南芥的野生型(ws)、异三聚体G蛋白α亚基基因GPA1缺失突变体(gpa11和gpa12)和超表达突变体(wGα和cGα)为材料,通过施加不同质量浓度(0-0.010 mg·L-1)的2,4D处理,对拟南芥根生长发育的形态指标进行了测定。结果表明(1)随着培养基中2,4D质量浓度的不断增大,5种基因型主根的伸长生长都受到抑制,且抑制作用随浓度升高而增强;4种突变体和野生型主根的生长在相同浓度2,4D处理下,无明显差异;(2)2,4D在一定浓度范围内,对拟南芥侧根的生长发育起促进作用;在2,4D诱导的侧根生长中,相同浓度2,4D处理下,缺失突变体明显比野生型侧根多,超表达突变体比野生型侧根少;gpa11和gpa12分别在2,4D质量浓度为0.005 mg·L-1和0.008 mg·L-1时的侧根数目与野生型差异最大。初步证明G蛋白不参与2,4D诱导的拟南芥主根生长发育的调节,而在侧根生长发育中可能起负调节作用。
Abstract:
Wild type (ws) Arabidopsis thaliana, two null mutants of G protein α subunit gene (gpα11 and gpα12) and two overexpressed mutants of G protein α subunit gene (wGα and cGα) were treated with 2,4D at different concentrations (0-0.010 mg·L-1) to comparatively examine some morphological parameters related to growth and development of the roots. It was observed that (1) increasing concentration of 2,4D inhibited elongation of the taproots in a dosedependent way. No differences were recorded between the ws and the four mutants; (2) within a certain range of concentration, 2,4D showed promoting effect on the growth and development of the lateral roots. Under the same concentration of 2,4D the numbers of the lateral roots were higher in null mutants and lower in overexpressed mutants, with reference to the wild type. The numbers of the lateral roots had the highest differences between the gpa12,gpa11 and the ws at 0.005 mg·L-1 and 0.008 mg·L-1 2,4D concentrations.It is concluded that the α subunit of G protein had no effect on the grow of taproot promoted by 2,4D, while playing a negative regulation role in the growth of lateral roots.

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备注/Memo

备注/Memo:
收稿日期:20060510修回日期:20060628基金项目:河南省自然科学基金项目(0624050023)作者简介:苏丽媛(1981),女,陕西宝鸡人,在读硕士,主要从事植物生理学研究。*通讯作者:梁宗锁,教授,博士生导师。Email : Liangzs@ms.iswc.ac.cn
更新日期/Last Update: 2010-05-04