[1]李俊红,张焕玲,李周岐.杜仲组织培养再生体系的优化[J].西北林学院学报,2008,23(04):97.
　LI Jun hong,ZHANG Huan ling,LI Zhou qi*.ptimization of Tissue Culture Regeneration System of Eucommia ulmoides Oliv.[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(04):97.
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杜仲组织培养再生体系的优化()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第23卷
期数:
2008年04期
页码:
97
栏目:
林木遗传育种
出版日期:
2008-07-30

文章信息/Info

Title:
ptimization of Tissue Culture Regeneration System of Eucommia ulmoides Oliv.
作者:
李俊红; 张焕玲; 李周岐
西北农林科技大学 林学院，陕西 杨陵 712100
Author(s):
LI Junhong; ZHANG Huanling; LI Zhouqi*
College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China
关键词:
杜仲; 组织培养; 再生体系; 优化
Keywords:
Eucommia ulmoides Oliv;  tissue culture;  regeneration syetem;  optimization
分类号:
S723.132
文献标志码:
A
摘要:
以幼嫩叶片为外植体，对杜仲再生体系的优化进行了研究。结果表明：叶片诱导愈伤组织的最适宜培养基为MS + NAA1.5 mg/L + BA2.0 mg/L，诱导率为95%、愈伤组织增殖最适宜培养基为MS+NAA1.0 mg/L +BA2.0 mg/L，良好率为98.5%、诱导不定芽的最适宜培养基为MS+ NAA0.5 mg/L +BA2.0 mg/L，分化率为40%、最适宜生根培养基1/2MS+IBA1.5 mg/L，生根率为92.5%。
Abstract:
Optimization of regeneration system of Eucommia ulmoides Oliv. was studied by using tender leaves as explants. The results showed that MS+NAA1.5 mg/L +BA2.0 mg/L had the best effects on induceing callus with a inducing rate of 95%, the best medium of callus growth was MS+NAA1.0 mg/L +BA2.0 mg/L with a conserve rate of 98.5%, the suitable medium for bud formation was MS medium added NAA0.5 mg/L, BA2.0 mg/L, with a differentiation rate of 40%, the optimum media for rooting is 1/2MS＋IBA1.5 mg/L with 92.5% rooting ratio.

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备注/Memo

备注/Memo:
收稿日期：20071225修回日期：20080411 基金项目：陕西省农业科技攻关项目“高产优质杜仲多倍体新品种选育”（2005K01G144）。作者简介：李俊红，男，西北农林科技大学林木遗传育种专业硕士研究生。 *通讯作者：李周岐，男，教授，博士生导师。
更新日期/Last Update: 2008-07-30