[1]王家明,张喜庆,田晓敏,等. 雷公藤属内生真菌研究（Ⅱ） 昆明山海棠内生真菌的分离鉴定及抗肿瘤活性菌株筛选[J].西北林学院学报,2012,27(03):109-112.
　WANG Jia-ming,ZHANG Xi-qing,TIAN Xiao-min,et al. Study on the Endophytic Fungi of Tripterygium（Ⅱ） Isolation of Endophytic Fungi from T. hypoglaucum and Screening of Strains Exhibited Antitumor Activities[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(03):109-112.
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 雷公藤属内生真菌研究（Ⅱ） 昆明山海棠内生真菌的分离鉴定及抗肿瘤活性菌株筛选()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第27卷
期数:
2012年03期
页码:
109-112
栏目:
出版日期:
2012-05-31

文章信息/Info

Title:
 Study on the Endophytic Fungi of Tripterygium（Ⅱ） Isolation of Endophytic Fungi from T. hypoglaucum and Screening of Strains Exhibited Antitumor Activities
文章编号:
1001-7461(2012)03-0109-04
作者:
 王家明;  张喜庆;  田晓敏;  王彩骄;  杜万富;  孙华; 王永禄; 李敬华
 (1.工业发酵微生物教育部重点实验室，天津科技大学 生物工程学院，天津 300457; 2.南京工业大学 药学院，江苏 南京 210000; 3.中国中医科学院 中医药信息研究所，北京 100700)
Author(s):
 WANG Jia-ming;  ZHANG Xi-qing;  TIAN Xiao-min;  WANG Cai-jiao;  DU Wan-fu;  SUN Hua;  WANG Yong-lu;  LI Jing-hua
 (1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; 2. School of Pharmaceutical Sciences, Nanjing University of Technology, Nanjing，Jiangsu 210000, China; 3. Institute of Information on Traditional Chinese Medicine, Chinese Academy of Chinese Medical Sciences, Beijing 100700, China)
关键词:
 ; 昆明山海棠; 内生真菌; 抗肿瘤活性; MTT法
Keywords:
 ; Tripterygium hypoglaucum;  endophytic fungi;  anti-tumor activity;  MTT assay
分类号:
S718.81
文献标志码:
A
摘要:
 为从昆明山海棠根及茎中分离鉴定内生真菌，并对其发酵产物乙酸乙酯提取物进行体外抗肿瘤活性筛选，采用微生物纯化方法，从昆明山海棠植物中分离得到内生真菌, 根据菌落形态和显微特征对菌株进行分类鉴定。选用Bel-7402（人肝癌细胞）、A549（人肺癌细胞）、BGC823（人胃癌细胞）、HCT-8（人结肠癌细胞）为指标，采用MTT法对发酵产物进行体外抗肿瘤活性筛选。结果表明，从昆明山海棠不同器官中分离得到37株内生真菌，经形态观察鉴定为2目、4科、8属。其中3株内生真菌发酵产物对不同肿瘤细胞具有显著选择性细胞毒作用。研究结果为国内首次从昆明山海棠植物中分离得到内生真菌，昆明山海棠内生真菌种类多样，具有潜在的研究和应用价值。

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