[1]崔秋华,孙永玉,段明伦,等. 齿瓣石斛无菌萌发条件优化研究[J].西北林学院学报,2012,27(05):106-110.
　CUI Qiu-hua,SUN Yong-yu,DUAN Ming-lun,et al. Optimization of Sterile Germination Conditions for Dendrobium devonianum[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(05):106-110.
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 齿瓣石斛无菌萌发条件优化研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第27卷
期数:
2012年05期
页码:
106-110
栏目:
出版日期:
2012-09-20

文章信息/Info

Title:
 Optimization of Sterile Germination Conditions for Dendrobium devonianum
文章编号:
1001-7461(2012)05-0106-05
作者:
 崔秋华; 孙永玉; 段明伦; 李昆; 黄传响; 闫红
 （1.中国林业科学研究院 资源昆虫研究所，云南 昆明 650224；2.云南龙陵县林业局，云南 龙陵678300; 3.攀枝花市林业局，四川 攀枝花617000）
Author(s):
 CUI Qiu-hua;  SUN Yong-yu;  DUAN Ming-lun;  LI Kun;  HUANG Chuan-xiang;  YAN Hong
 (1. Research Institute of Resource Insects, CAF, Kunming,Yunnan 650224，China；2. Forest Bureau of Longling County，Longling，Yunnan 678300，China；3. Panzhihua Forestry Bureau，Panzhihua，Sichuan 617000, China)
关键词:
 ; 齿瓣石斛; 无菌萌发; 条件优化
Keywords:
Dendrobium devonianum;  sterile germination;  optimization
分类号:
S723.1
文献标志码:
A
摘要:
 为优化齿瓣石斛无菌萌发条件，通过研究影响齿瓣石斛种子萌发的因子，探寻适合齿瓣石斛种子最优萌发的培养基及培养条件。结果表明：齿瓣石斛种子无菌培养的适宜培养基为MS培养基,蔗糖浓度为20 g/L,浓度过低，萌发苗生长差，玻璃化严重；胚龄对齿瓣石斛的萌发率有重要影响，适宜的种子胚龄为10～11个月，胚龄时间短萌发率低；胚龄时间过长灭菌时蒴果易开裂，污染率大；添加0.3 mg/L NAA，可缩短萌发时间，种子的萌发率、幼苗的成苗率和生长情况相比对照较好；不同培养条件萌发效果有明显的差异。适宜的萌发条件可促进齿瓣石斛萌发，提高萌发率，萌发后幼苗生长状况较好。

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