[1]李永丽,周洲,范晶超,等. 2001杨组培再生体系建立[J].西北林学院学报,2012,27(06):83-87.
　LI Yong-li,ZHOU Zhou,FAN Jing-chao,et al. Establishment of Regeneration System for Populus ×euramericana cv.‘2001’[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(06):83-87.
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 2001杨组培再生体系建立()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第27卷
期数:
2012年06期
页码:
83-87
栏目:
出版日期:
2012-11-30

文章信息/Info

Title:
 Establishment of Regeneration System for Populus ×euramericana cv.‘2001’
文章编号:
1001-7461(2012)06-0083-05
作者:
 李永丽; 周洲; 范晶超; 郑涛
 (1.河南科技大学 林学院，河南 洛阳 471003; 2.林木育种国家工程实验室，林木花卉遗传育种教育部重点实验室，北京林业大学，北京100083; 3.西北农林科技大学 林学院, 陕西 杨陵 712100)
Author(s):
 LI Yong-li;  ZHOU Zhou;  FAN Jing-chao;  ZHENG Tao
 (1.College of Forestry, Henan University of Science and Technology, Luoyang, Henan 471003, China； 2. National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 100083, China；3. College of Forestry，Northwest A&F University, Yangling, Shaanxi 712100，China）
关键词:
 ; 欧美杨; 离体培养; 芽伸长; 生根
Keywords:
 ; Populus×euramericana;  in vitro culture;  buds elongation;  rooting
分类号:
S792.11
文献标志码:
A
摘要:
 以2001杨的春季新发嫩枝茎段、叶柄、叶片为外植体，尝试在MS和WPM基本培养基中添加多种组合激素，探索不定芽诱导、丛生芽伸长和生根最优条件，建立组织培养与植株再生系统。结果表明：MS培养基的诱导分化效果比WPM好；在MS+TDZ 0.025 mg·L-1+6-BA 0.05 mg·L-1+NAA 0.05 mg·L-1+蔗糖30.00 g·L-1的培养基上，不定芽诱导分化效果最好，茎段和叶柄诱导分化率可达90%，叶片诱导分化率 60%；丛生芽转入MS+KT 0.25 mg·L-1+GA3 1.00 mg·L-1+果糖30.00 g·L-1的培养基中茎伸长效果最佳；每个叶片外植体诱导茎伸长的芽平均达6.4个，芽数量最多；不定芽在1/2MS+IAA 0.02 mg·L-1+IBA 0.02 mg·L-1+AC 3.00 g·L-1+蔗糖30.00 g·L-1培养基上形成的根条数多，生根率达90%。

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