[1]杨柳,陆军,朱杰丽,等. 基质分散萃取—反相高效液相色谱法测定猕猴桃中氯吡脲[J].西北林学院学报,2013,28(02):156-159.[doi:10.3969/j.issn.1001-7461.2013.02.30]
　YANG Liu,LU Jun,ZHU Jie-li,et al. Determination of Forchlorfenuron in Kiwi Fruit by Matrix Solid Phase Dispersion Extraction and Reversed High Performance Liquid Chromatography[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(02):156-159.[doi:10.3969/j.issn.1001-7461.2013.02.30]
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 基质分散萃取—反相高效液相色谱法测定猕猴桃中氯吡脲()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第28卷
期数:
2013年02期
页码:
156-159
栏目:
出版日期:
2013-03-30

文章信息/Info

Title:
 Determination of Forchlorfenuron in Kiwi Fruit by Matrix Solid Phase Dispersion Extraction and Reversed High Performance Liquid Chromatography
文章编号:
1001-7461(2013)02-0156-04
作者:
 杨柳; 陆军; 朱杰丽; 吴翠蓉; 柴振林; 周善森
 (1.浙江省林产质量检测站，浙江 杭州 310023; 2.龙泉市林业科学研究所，浙江 龙泉 323700)
Author(s):
 YANG Liu; LU Jun; ZHU Jie-li; WU Cui-rong; CHAI Zhen-lin; ZHOU Shan-sen
 (1.Zhejiang Forestry Product Test Station,Hangzhou ,Zhejiang 310023,China;2.Longquan Forestry Institute,Longquan, Zhejiang 323700，China)
关键词:
 ; 基质分散萃取; 反相高效液相色谱; 猕猴桃; 氯吡脲; 测定
Keywords:
 ; MSPD;  RP-HPLC;  kiwi fruit;  forchlorfenuron;  detect
分类号:
S663.4
DOI:
10.3969/j.issn.1001-7461.2013.02.30
文献标志码:
A
摘要:
 建立了基质分散萃取-反相高效液相色谱法测定猕猴桃中氯吡脲的方法：以猕猴桃为研究材料，乙腈为提取剂，甲醇为淋洗液、经氨基柱净化后，经高效液相色谱检测。结果表明：以甲醇/水（67/33，V/V）1 mL·min-1为流动相，经Kromasil C18(250 mm×4.6 mm，5 μm锈钢柱、UV265 nm进行待测组分的分离和测定效果最佳，检测线性范围0.025~100 μg·mL-1，相关系数r=0.999 9，添加低、中、高浓度的氯吡脲回收率分别为108%、98.3%、97.9%，相对标准偏差分别为2.1%、0.27%、0.23%，方法检出限为0.01 mg·kg -1。该方法测定猕猴桃氯吡脲含量准确可靠。
Abstract:
 Forchlorfenuron,a residue of plant growth regulator in kiwi fruit was determined by matrix solid phase dispersion extraction (MSPD) and reversed high performance liquid chromatography (RP-HPLC).Kiwi fruit was extracted with acetonitrile.The extract was pre-purified by amino column eluted by methanol.The resultant extract was then separated and measured by HP LC.The optimum conditions of HPLC were as follows: mobile phase: methanol/water (67/33,V/V) with a flow rate of 1.0 mL·min-1,column: Kromasil C18 (250 mm×4.6 mm，5 μm),and UV detection wavelength:265 nm. External standard calibration was adopted for quantification.The linearity was good when the concentration ranged from 0.125 to 100 μg·mL-1 (r=0.999 9).Recovery rates at low,medium and high concentrations of the addition of forchlorfenuron were 108%,98.3%,and 97.9%,with the relative standard deviations of 2.1%，0.27%，and 0.23%,respectively.The limit of detection was 0.01 mg·kg-1.The method is accurate and reliable for the determination of forchlorfenuron in kiwi fruit.

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备注/Memo

备注/Memo:
 收稿日期：2012-06-08 修回日期：2012-09-28
基金项目：浙江省省院合作林业科技项目（2011SY03）;浙江省分析测试基金项目（2011F82G1450001）。
作者简介：杨柳，男，工程师，硕士，研究方向：食品安全。E-mail：yangliuxs@163.com
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