[1]魏宁,樊军锋,杨培华,等. 油松苯丙氨酸解氨酶基因的克隆与序列分析[J].西北林学院学报,2013,28(03):98-103.[doi:10.3969/j.issn.1001-7461.2013.03.18]
 WEI Ning,FAN Jun-feng,YANG Pei-hua,et al. Molecular Cloning and Sequence Analysis of Phenylalanine Ammonia-lyase Gene in Pinus tabulaeformis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(03):98-103.[doi:10.3969/j.issn.1001-7461.2013.03.18]
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 油松苯丙氨酸解氨酶基因的克隆与序列分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第28卷
期数:
2013年03期
页码:
98-103
栏目:
出版日期:
2013-05-30

文章信息/Info

Title:
 Molecular Cloning and Sequence Analysis of Phenylalanine Ammonia-lyase Gene in Pinus tabulaeformis
文章编号:
1001-7461(2013)03-0098-06
作者:
 魏宁樊军锋杨培华刘永红刘春英
 (西北农林科技大学 林学院,陕西 杨陵 712100)
Author(s):
 WEI Ning FAN Jun-feng YANG Pei-hua LIU Yong-hong LIU Chun-ying
 (College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China)
关键词:
 油松苯丙氨酸解氨酶克隆序列分析
Keywords:
Pinus tabulaeformis phenylalanine ammonia-lyase (PAL) cloning sequence analysis
分类号:
S791.254
DOI:
10.3969/j.issn.1001-7461.2013.03.18
文献标志码:
A
摘要:
 利用同源克隆的方法,从油松(Pinus tabulaeformis)中克隆得到苯丙氨酸解氨酶(PAL)基因的cDNA和DNA序列,cDNA序列全长2 157 bp,编码718个氨基酸,包含有完整的ORF,命名为TaPAL(GenBank登录号:JX280460)。通过核苷酸序列多重比较表明,TaPAL基因与其他植物PAL基因高度同源,预测的蛋白序列包含与水稻、玉米PAL相同的脱氨基位点和催化活性位点。PAL系统进化树显示,TaPAL与松科类植物PAL聚类关系最近。另外,对比发现TaPAL基因组DNA序列与cDNA序列完全一样,表明该基因无内含子。
Abstract:
 The cDNA and DNA sequence of phenylalanine ammonia-lyase gene was cloned from Pinus tabulaeformis for the first time by the method of homology cloning. The full length of cDNA was 2 157 bp, containing a complete open reading frame and 718 amino acids were encoded, designated as TaPAL(Accession number:JX280460). It was indicated that the nucleic acid sequence of TaPAL shared a high identity with other plants’ via multiple alignment. The domination sites and catalytic active sites appeared in PAL protein of Oryza and Zea mays were also found in that of inferred protein sequence. Phylogenetic tree analysis suggested that TaPAL was much closer to PALs from Pinacease than that from other plants. Moreover, this gene was found to be intronless because the cDNA and genomic DNA sequence of TaPAL were completely the same.

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[4]高爱琴,梁英梅,吐拉布比.松苗立枯病防治试验[J].西北林学院学报,1999,14(03):0.
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[5]谢斌,郭俊荣,杨培华,等.油松无性系种子园疏伐研究[J].西北林学院学报,1999,14(03):0.
 Xie Bin,Guo Junrong,Yang Peihua,et al.On Thinning of Clone Seed Orchard of Pinus tabulaeformis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,1999,14(03):0.
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 Zhao Rongjun,Yang Peihua,Xie Bin,et al.A Study on Wood Structure and Physical and Mechanical Properties of the Half-Sib Progeny and Parent of Pinus tabulaeformis Carr[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2000,15(03):24.
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 Effect of Reserve Density on Growth of Aerial Seeding of Pinus tabulaeformis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2003,18(03):57.
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 ZHANG Hai-feng,WANG Hong-zhe,WANG Quan-bo,et al. Damaging Regularity of Red Turpentine Bark Beetle (Scolytidae: Dendroctonus valens Le Conte) In Northwest China[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(03):164.
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备注/Memo

备注/Memo:
 收稿日期:2013-02-03〓修回日期:2013-03-19
基金项目:林业公益性行业科研专项(200704039)。
作者简介:魏宁,男,在读硕士,研究方向:油松遗传改良。E-mail:weining1203@163.com
*通信作者:樊军锋,男,博士,研究员,研究方向:杨树、油松基因工程、新品种选育与遗传改良。E-mail:fanjf28@163.com
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