[1]任耀忠,张文辉,周建云. 栓皮栎不同变异类型RAPD分析[J].西北林学院学报,2013,28(04):66-70.[doi:10.3969/j.issn.1001-7461.2013.04.13]
 REN Yao-zhong,ZHANG Wen-hui,ZHOU Jian-yun. RAPD Analysis on Different Variant Types of Quercus variabilis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(04):66-70.[doi:10.3969/j.issn.1001-7461.2013.04.13]
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 栓皮栎不同变异类型RAPD分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第28卷
期数:
2013年04期
页码:
66-70
栏目:
出版日期:
2013-07-30

文章信息/Info

Title:
 RAPD Analysis on Different Variant Types of Quercus variabilis
文章编号:
1001-7461(2013)04-0066-05
作者:
 任耀忠张文辉周建云
 (1.西北农林科技大学 西部环境与生态教育部重点实验室,陕西 杨陵 712100;2.四川省林木种苗站, 四川 成都 610081)
Author(s):
 REN Yao-zhongZHANG Wen-huiZHOU Jian-yun
 (1.Key Laboratory of Environment and Ecology of Education Ministry in West China,Northwest A&F University,Yangling,Shaanxi 712100,China; 2.Forest Seedling Station of Sichuan Province,Chengdu,Sichuan 610081,China)
关键词:
 栓皮栎变异类型RAPD遗传多样性
Keywords:
Quercus variabilis variant type RAPD genetic diversity
分类号:
S722.31
DOI:
10.3969/j.issn.1001-7461.2013.04.13
文献标志码:
A
摘要:
 运用RAPD技术从235条随机引物中筛选出35条引物,对取自秦岭北坡4个类型的24份天然栓皮栎种群的材料基因组DNA进行扩增。结果表明:栓皮栎天然群体的总体遗传多样性水平高,栓皮栎天然群体遗传分化大、遗传变异高;通过UPGMA聚类分析,将栓皮栎天然群体划分成厚皮深裂型、厚皮浅裂型、薄皮浅裂型、薄皮深裂型4个变异类型是有遗传基础的,其与形态学分类基本一致;多态位点数、多态百分率、观测等位基因平均数、Shannon信息指数和Nei基因多样性指数所显示的各类型遗传多样性变化规律都一致,即厚皮深裂型>薄皮浅裂型>薄皮深裂型>厚皮浅裂型,各变异类型的遗传多样性水平高;栓皮栎遗传种质资源极其丰富,遗传改良潜力大。
Abstract:
 In order to speed up the genetic improvement of Quercus variabilis,genomic DNAs of 24 materials of four types collected from the northern slope of Qinling Mountains were amplified by RAPD ( random amplified polymorphic DNA) technology with 35 primers screened from 235 arbitrary primers.The total genetic diversity level of natural Q.variabilis population was high,the genetic differentiation of natural Q.variabilis population was great and its genetic variation was high.The natural population of Q.variabilis was divided into four variant types: thick-bark-deep-crack type,thick-bark-shallow-crack type,thin-bark-shallow-crack type,and thin-bark-deep-crack type.The genetic basis of the division was demonstrated by UPGMA cluster analysis,which coincided with its morphological taxonomy basically.Polymorphic loci,percentage of polymorphic loci,average observed number of alleles,Shannon information index and Nei’s genetic diversity indicated that the changes of genetic diversity of all types were consistent:thick-bark-deep-crack type>thin-bark-shallow-crack type>thin-bark-deep-crack type>thick-bark-shallow-crack type,and their genetic diversity was high.Genetic germplasm resources were extremely abundant and genetic improvement potentials were great in Q.variabilis.

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 ZHOU Jianyun,CAO Xuping,ZHANG Hongbo,et al.Classification of Natural Types of Quercus variabilis in Shaanxi Province[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2009,24(04):16.
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 REN Yao zhong,ZHANG Wen hui*,LIU Li li,et al.Optimization of RAPD in Quercus variabilis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2009,24(04):55.
[3]张丽丛,雷亚芳,常宇婷.栓皮栎软木主要化学成分的分析[J].西北林学院学报,2009,24(04):163.
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 XIN Fumei,ZHANG Cunxu,WANG Hong.Antioxides Characteristics of Embryogenic Callus and Nonembryogenic Callus in Quercus variabilis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(04):67.
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 DING Huiping,QIAN Kehong,YANG Yunjing.Fractal Properties of the Spatial Pattern of Quercus variabilis Population in Shaanxi[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(04):7.
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 WANG Di-hai,ZHAO Zhong,ZHANG Yan. The Fine Root of Robinia pseudoacacia and Soil Moisture in the Loess Plateau[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(04):1.
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 YANG Yang,ZHANG Cun-xu,HAO Li-li,et al. Establishment of AFLP Reaction System in Quercus variabilis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(04):66.
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 JI Zhi-qing,ZHANG Cun-xu,ZHANG Chang-sheng,et al. Cryopreservation of Embryogenic Tissues in Quercus variabilis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(04):88.
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 YUAN Yi-dan,ZHU Ling-yan,SONG Xiao-zhou*. Cellular Structure and Principal Characteristics of the Cork from Quercus variabilis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(04):216.[doi:10.3969/j.issn.1001-7461.2017.03.40]
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备注/Memo

备注/Memo:
 收稿日期:2012-12-12 修回日期:2013-01-13
基金项目:国家林业局林业公益性行业专项(201004011);国家自然科学基金项目(30872018);陕西省重点基础研究项目(2009JZ005)。
作者简介:任耀忠,男,硕士,研究方向:森林培育和林木遗传育种。E-mail:305217778@qq.com
*通信作者:张文辉,男,博士,教授,研究方向:植物分类、植物生态。E-mail:xlzhao@public.xa.sn.cn
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