[1]宋要强,张 琳,惠 伟*.一氧化氮和乙烯在打破杜梨种子休眠中的相互作用[J].西北林学院学报,2013,28(06):51-55.[doi:10.3969/j.issn.1001-7461.2013.06.10]
　SONG Yao-qiang,ZHANG Lin,HUI Wei*.The Interaction of Nitric Oxide and Ethylene in Breaking the SeedDormancy of Pyrus betulifolia [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(06):51-55.[doi:10.3969/j.issn.1001-7461.2013.06.10]
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一氧化氮和乙烯在打破杜梨种子休眠中的相互作用()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第28卷
期数:
2013年06期
页码:
51-55
栏目:
出版日期:
2013-11-30

文章信息/Info

Title:
The Interaction of Nitric Oxide and Ethylene in Breaking the SeedDormancy of Pyrus betulifolia
文章编号:
1001-7461(2013)06-0051-05
作者:
宋要强1; 2; 张 琳1; 惠 伟1*
1.陕西师范大学 生命科学学院,陕西 西安 710062;
2.陕西化龙山国家自然保护区管理局, 陕西 镇坪 725600
Author(s):
SONG Yao-qiang1; 2;  ZHANG Lin1;  HUI Wei1*
1.College of Life Sciences,Shaanxi Normal University, Xi’an, Shaanxi 710062, China;
2.Hualongshan Nationgal Nature Reserve of Shaannxi,Zhenping, Shaanxi 725600, China
关键词:
杜梨种子;  休眠;  一氧化氮;  乙烯
Keywords:
Pyrus betulifolia seed;  dormancy;  NO;  ethylene
分类号:
S758
DOI:
10.3969/j.issn.1001-7461.2013.06.10
文献标志码:
A
摘要:
为一氧化氮(NO)和乙烯(ET)在打破杜梨种子休眠中的作用及信号关系,用硝普钠(SNP)、乙烯利(ETH)、NO清除剂PTIO、ET作用抑制剂1-甲基环丙烯(1-MCP)等处理休眠的杜梨种子后,在20℃下用培养皿滤纸法培养,观察种子萌发率。结果表明,NO和ET均能有效地打破杜梨种子休眠,其中10 mmol·L-1SNP和2 mmol·L-1 ETH 24 h 浸泡处理使杜梨种子14 d时的发芽率分别达到55.7%和57.8%,与对照相比差异显著(p<0.05),PTIO具逆转SNP作用的特性,1-MCP具逆转ETH作用的特性; 经SNP和ETH处理后,可显著提高杜梨种子内源ET的释放速率,分别使杜梨种子第4天ET的释放速率达到0.14 μL·kg-1·h-1和0.15 μL·kg-1·h-1,显著高于对照(0.04 μL·kg-1·h-1),1-MCP可以逆转SNP打破休眠的作用,但是PTIO不能逆转ETH的作用。说明NO和ET都能打破杜梨种子休眠,NO处在ET信号通路的上游,通过促进内源ET的形成来打破杜梨种子休眠。
Abstract:
The effects and signal relationships of nitric oxide(NO)and ethylene on breaking the seed dormancy of Pyrus betulifolia were examined. The dormancy seeds were treated with sodium nitroprusside(SNP), ethephon(ETH), NO scavenger 2-phenyl-4, 4, 5, 5-tetramethylimidazo-line-1-oxyl-3-oxide(PTIO), and ethylene inhibitor 1-methylcyclo-propene(1-MCP)individually or with the combination. The treated seeds were then trained by dish-filter method at 20℃, and the seed germination rate was observed. The results indicated that both NO and ET could effectively break the seed dormancy, by which the germination rates of 24-hour pretreated seeds treated by 10 mmol·L-1SNP or 2 mmol·L-1 ETH were 55.7% and 57.8%, respectively, significantly different with the control(p<0.05). PTIO reversed the role of SNP, and 1-MCP reversed the role of ETH. By the treatment of SNP and ETH, endogenous ethylene production of the seeds significantly increased, the release rates of ethylene reached 0.14 μL·kg-1·h-1 and 0.15 μL·kg-1·h-1, respectively at the 4th day of the treatment by SNP and ETH, significantly higher than that of the control(0.04 μL·kg-1·h-1). 1-MCP could reverse the above effects of SNP, however, no evidence were found for PTIO to reverse the effects of ETH, indicating that the either NO or ethylene could break the seed dormancy, and NO was at the up stream of ethylene signaling pathway, involving the stimulation of ethylene production in breaking P. betulifolia seed dormancy.

参考文献/References:

[1] WENDEHENNE D, DURNER J, KIESSIG D F. Nitric oxide: a new player in plant signaling and defence responses[J]. Current Opinion in Plant Biology, 2004, 7(4):449-455.
[2] CRAWFORD N M, GUO F Q. New insights into nitric oxide metabolism and regulatory functions[J].Trends in Plant Science, 2005, 10(4):195-200.
[3] LAMOTTE O, COURTOIS C, BARNAVON L, ET AL. Nitric oxide in plants: the biosynthesis and cell signaling properties of a fascinating molecule[J]. Planta, 2005, 221(1):1-4
[4] BELIGNI M V, LAMATTINA L. Nitric oxide stimulates seed germination and deetiolation, and inhibits hypocotyl elongation, three light-inducible responses in plants[J]. Planta, 2000, 210(2): 215-221.
[5] BETHKE P C, BADGER M R, JONES R L. Apoplastic synthesis of nitric oxide by plant tissues [J]. The Plant Cell, 2004, 16(2): 332-341.
[6] SARATH G, HOU G C, BAIRD L M.et al.Reactive oxygen species, ABA and nitric oxide interactions on the germination of warm-season C4-grasses [J]. Planta, 2007, 226(3): 697-708.
[7] GNIAZDOWSKA A, DOBRZYSKA U,BABáNCY K T, et al.Breaking the apple embryo dormancy by nitric oxide in volves the stimulation of ethylene production[J]. Planta, 2007, 225(4): 1051-1057.
[8] CALVO A P, NICOL A S C,LORENZO O,et al. Evidence for positive regulation by gibberellins and ethylene of ACC oxidase expression and activity during transition from dormancy to germination in Fagus sylvatica L. seeds[J]. Plant Growth Regulation, 2004, 23(1): 44-53.
[9] RIBEIRO D M,Barros R S.Germination of dormant seed of Stylosanthes humilis as promoted by ethylene accumulation in closed environments[J].Brazilian Journal of Plant Physiology, 2004(16): 83-88.
[10] 高俊凤. 植物生理学实验指导[M]. 北京:高等教育出版社,2006:196-198.
[11] 胡晋. 种子生物学[M]. 高等教育出版社,2006, 1:126-140.
[12] GRUBISIC D, GIBA Z, KONJEVIC R.The effect of organic nitrates in phytochrome controlled germination of Paulownia tomentosa seeds[J]. Photochemisry Photobiology, 1992, 56(5): 629-632.
[13] LINDERMAYR C, SAALBACH G, BAHNWEG G, et al. Differential inhibition of Arabidopsis methionine adenosylt ransferases by protein S nitrosylation[J].The Journal of Biology Chemistry, 2006, 281(7): 4285-4291.
[14] NICOLAS C, NICOLAS G, RODRI GUEZ D. Antagonistics effects of abscisic acid and gibberellic acid on the breaking of dormancy of Fagus sylvatica seeds[J]. Physiologia Plantarum, 1996, 96(2): 244-250.
[15] ORACZL K,El-MAAROUF-BOUTEAU H,BOGATEK R,et al. Release of sunflower seed dormancy by cyanide: cross-talk with ethylene signalling pathway[J]. Journal of Experimental Botany, 2008, 59(8): 2241-2251.
[16] ZHANG L G, WANG Y D, ZHAO L, et al. Involvement of nitric oxide in light-mediated greening of barley seedlings[J]. Plant Physiology, 2006, 163(8): 818-826.
[17] MATILLA A J. Ethylene in seed formation and germination[J]. Seed Science Research, 2000, 10(2): 111-126.
[18] PARANI M, RUDRABHATLA S, MYERS R. et al. Microarray analysis of nitric oxide responsive transcripts in Arabidopsis[J]. Plant Biotechnology Journal, 2004, 2(4): 359-366.

备注/Memo

备注/Memo:
收稿日期:2013-03-07 修回日期:2013-04-25
基金项目:陕西省农业科技创新项目(2012NKC01-22)。
作者简介:宋要强,男,硕士,助理工程师,研究方向:植物学。E-mail:songyaoqiang-888@163.com
*通信作者:惠伟,女,副教授,研究方向:植物生理.E-mail:huihui@snnu.edu.cn
更新日期/Last Update: 2013-11-30