[1]杨恩让,刘晓敏,解 庆.山桐子离体培养植株再生[J].西北林学院学报,2013,28(06):95-98.[doi:10.3969/j.issn.1001-7461.2013.06.18]
 YANG En-rang,LIU Xiao-min,XIE Qing.Establishment of Plantlet Regeneration System of Idesia polycarpa in vitro[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(06):95-98.[doi:10.3969/j.issn.1001-7461.2013.06.18]
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山桐子离体培养植株再生()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第28卷
期数:
2013年06期
页码:
95-98
栏目:
出版日期:
2013-11-30

文章信息/Info

Title:
Establishment of Plantlet Regeneration System of Idesia polycarpa in vitro
文章编号:
1001-7461(2013)06-0095-04
作者:
杨恩让1刘晓敏2解 庆2
1.宝鸡市林业科学研究所,陕西 宝鸡 721000;
2.西北农林科技大学,林学院 陕西 杨陵 712100
Author(s):
YANG En-rang1 LIU Xiao-min2 XIE Qing2
1 Baoji Forestry Science Research Institute, Baoji, Shaanxi 721000, China;
2. College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China
关键词:
山桐子 组织培养 再生体系
Keywords:
Idesia polycarpa tissue culture regeneration system
分类号:
S722.3
DOI:
10.3969/j.issn.1001-7461.2013.06.18
文献标志码:
A
摘要:
以种子和叶片为外植体,研究了离体条件下外植体类型及植物生长调节剂对山桐子器官发生和植株再生的影响。结果表明:种子为诱导愈伤组织的适宜外植体; 在MS+1.0 mg·L-16-BA +1.0 mg·L-1 NAA +3%蔗糖+0.6%琼脂的培养基上,愈伤诱导率达96.8%; 继代培养在MS+0.08 mg·L-1 TDZ + 1.5 mg·L-16-BA +0.05 mg·L-1 NAA 培养基上能成功的诱导芽的分化,诱导率达48.3%以上; 芽增殖的适宜培养基为1/2MS +0.03 mg·L-1 TDZ +2.0 mg·L-16-BA +0.1 mg·L-1 NAA +3%蔗糖+0.6%琼脂,增殖系数为4.83; 在1/2MS+ 0.5 mg·L-1 IBA +2%蔗糖+0.6%琼脂培养基上,无根苗生根率达88%。
Abstract:
Seeds and tender leaves of Idesia polycarpa were used as a source of initial explants. The effects of explants and plant growth regulators on organogenesis and plantlet regeneratio in vitro were studied. The results showed that seeds were more suitable for initial explants in inducing calli. High induction rate of 96.8% was achieved in MS medium supplemented with 1.0 mg·L-1 NAA,1.0 mg·L-1 6-BA, 3% sucrose and 0.6%agar. MS medium supplemented with 0.08 mg·L-1 TDZ, 1.5 mg·L-1 6-BA and 0.05 mg·L-1 NAA in subculture was in favor of the shoot differentiation and the rate was over 48.3%. The shoot proliferation coefficient reached 4.83 in 1/2MS medium with 0.03 mg·L-1TDZ, 2.0 mg·L-1 6-BA, 0.1 mg·L-1 NAA,3% sucrose and 6% agar. High rooting percentages and excellent development root systems were obtained on 1/2MS medium with 0.5 mg·L-1 IBA, 2% sucrose and 0.6% agar.

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备注/Memo

备注/Memo:
收稿日期:2012-05-15 修回日期:2013-08-26。
基金项目:陕西省科技攻关项目( 2007-K0111); 西安市科技计划项目(YF07087)。
作者简介:杨恩让,男,高级工程师,研究方向:林木种苗繁殖与森林培育。E-mail:yangenrang88@163.com
更新日期/Last Update: 2013-11-30