[1]郭娟,樊军锋*,梁军,等. 利用SRAP标记鉴别美洲黑杨及指纹图谱构建[J].西北林学院学报,2014,29(02):98-102.[doi:doi:10.3969/j.issn.1001-7461.2014.02.17]
 GUO Juan,FAN Jun-feng*,LIANG Jun,et al. Identification and Fingerprinting of Populus deltoides Using SRAP Markers[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(02):98-102.[doi:doi:10.3969/j.issn.1001-7461.2014.02.17]
点击复制

 利用SRAP标记鉴别美洲黑杨及指纹图谱构建()
分享到:

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年02期
页码:
98-102
栏目:
出版日期:
2014-03-30

文章信息/Info

Title:
 Identification and Fingerprinting of Populus deltoides Using SRAP Markers
文章编号:
1001-7461(2014)-02-0098-05
作者:
 郭娟1樊军锋1*梁军2李薇1
 (1.西北农林科技大学 林学院,陕西 杨陵 712100;2.中国林业科学研究院 森林生态环境与保护研究所,北京 100091)
Author(s):
 GUO Juan1FAN Jun-feng1*LIANG Jun2LI Wei1
 (1. College of Forestry , Northwest A&F University , Yangling , Shaanxi 712100 , China; 2. The Research Institute of Forest Ecology, Environment and Protection, The Chinese Academy of Forestry, Beijing 100091, China)
关键词:
 美洲黑杨SRAP品种鉴别指纹图谱
Keywords:
Populus deltoides SRAP variety identification fingerprinting analysis
分类号:
S792.110.4
DOI:
doi:10.3969/j.issn.1001-7461.2014.02.17
文献标志码:
A
摘要:
 利用SRAP分子标记对美洲黑杨I-69及与其有亲缘关系的4个美洲黑杨品种进行了分子鉴别与指纹图谱分析。结果表明:从72对SRAP引物组合中筛选出21对引物组合可以将5个品种完全区分开,21对引物共扩增出287条带,条带清晰,平均每对引物扩增条带为13.7条,多态性条带209条,多态性比率为72.8%,多态性高;各杨树品种之间的遗传相似系数在0.548 1~0.769 2之间,平均相似系数为0.658 7。并选用扩增出的61条特异性条带构建5个杨树品种的分子指纹图谱。聚类分析结果表明,5个美洲黑杨品种间遗传差异较小,其中陕林3号与北抗1号的遗传差异最大。
Abstract:
 Identification and molecular fingerprinting analysis of Populus deltoides including P. deltoides.cv. ’Lux’(I-69/55) and its 4 affinity varieties were conducted using SRAP makers. Twenty one pairs of primers were screened from 72 primer combinations that could produce polymorphic and high quality bands, and identify 5 varieties completely. Two hundreds and eighty seven bands were amplified and 209 bands were polymorphic from these 21 pairs of primers. Each pair of primers amplified 13.7 bands on average, and the percentage of polymorphic bands was 72.8%. 61 specific bands amplified were used for analysis of fingerprinting in the 5 varieties. The similarity coefficients of the five Populus varieties ranged from 0.548 1 to 0.769 2 with an average of 0.658 7. Cluster analysis showed that the genetic differentiation among these 5 P. deltoides varieties was low and P.deltoides. cv.’SL-3’ and P. deltoides.cv.’Beikang-1(16-27/92)’ showed the highest genetic differentiation among five varieties.

参考文献/References:

 [1] 李文文,黄秦军,昌俊,等.南方型和北方型美洲黑杨幼苗光合作用的日季节变化[J].林业科学研究,2010,23(2):227-233.LI W W, HUANG Q J, CHANG J, et al.Studies on photosynthetic characteristics of South and North-typed Populus deltoides Younglings[J]. Forest Research, 2010, 23(2):227-233.
[2]李世峰,张博,陈英,等.美洲黑杨种质资源遗传多样性的SSR分析[J].南京林业大学学报:自然科学版,2006,30(4):10-13.LI S F, ZHANG B, CHEN Y, et al.Analysis of genetic diversity of Populus deltoides germplasm by SSR[J]. Journal of Nanjing Forestry University:Nat. Sci. Edi.,2006, 30(4):10-13.(in Chinese)
[3]刘春英,樊军锋,高建社,等.杨树新杂种的SSR分析及鉴定[J].西北林学院学报,2013,28(2):70-73.LIU C Y, FAN J F, GAO J S, et al.SSR(simple sequence repeat) analysis and identify of new Populus hybrid[J]. Journal of Northwest Forestry University, 2013, 28(2):70-73.(in Chinese)
[4]肖哲丽.杨树ISSR指纹图谱构建及应用研究[D].银川:宁夏大学,2011.
[5]朱坚,高巍,林伯德,等.金针菇种质资源的SRAP分析[J].福建农林大学学报:自然科学版,2007,36(2):154-158.ZHU J, GAO W, LIN B D,et al.SRAP analysis of Flammulina velutipes germplasm resources[J]. Journal of Fujian Agriculture and Forestry University:Nat. Sci.Edi.,2007, 36(2):154-158.(in Chinese)
[6]LI G, OUIROS C F. Sequence-related amplified polymorphism(SRAP), a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Bmssica[J]. Theor.Appl.Genet., 2001, 103:455-46.
[7]RIAZ A, LI G, QURESH Z, et,al. Genetic diversity of oilseed Brassica napus inbred lines based on sequence-related amplified polymorphism and its relation to hybrid performance[J]. Plant Breeding, 2001, 120(5):411-415.
[8]FERRIOL M, PICO B, NUEZ F. Genetic diversity of a germplasm collection of cucurbita pepo using SRAP and AFL Pmarkers[J].Theor.Appl.Genet., 2003, 107(2):271-282.
[9]陈艺,郭军站,李辉,等.15个果桑无性系指纹图谱的构建[J].西北林学院学报,2011,26(6):62-65.CHEN Y, GUO J Z, LI H, et al.Fingerprints construction of 15 fruit mulberry (Morus spp.) clone[J]. Journal of Northwest Forestry University, 2011, 26(6):62-65.(in Chinese)
[10]高建明.杨属植物的ISSR与AFLP指纹分析及应用研究[D].天津:南开大学,2006.
[11]王源秀.响叶杨×银白杨遗传图谱构建及杨属图谱比较研究[D].南京:南京林业大学,2008.
[12]谭碧玥,王源秀,徐立安.杨树基因组SRAP扩增体系的建立与优化[J].林业科技开发,2009,23(2):25-29.TAN B Y, WANG Y X, XU L A. Establishment and optimization of SRAP-PCR reaction system of Populus[J]. China Forestry Science and Technology, 2009, 23(2):25-29.
[13]刘成平.瓠瓜EST-SSR、SRAP、ISSR和RAPD反应体系的建立及其纯度鉴定应用的研究[D].武汉:华中农业大学,2008.
[14]PANAUD O, CHEN X, MCCONCH S D. Development of microsatellite markers and characterization of simple saquence length polymorphism (SSLP)in rice(Oryza sativa)[J]. Mol. Gen. Genet., 1996, 252(5):597-607.
[15]陈罡,邢兆凯,潘文利,等.辽宁地区主栽杨树品种的SRAP标记遗传多样性分析[J].东北林业大学学报,2010, 38(10):19-22.CHEN G, XING Z K, PAN W L, et al.Genetic diversities of Populus in Liaoning area by using SRAP markers[J].Journal of Northeast Forestry University, 2010, 38(10):19-22.(in Chinese)
[16]FERRIOL M, PICO B, NUEZ F. Genetic diversity of some accession of Cucubita maxima from Spain using RAPD and SRAP markers[J]. Genetic Resources and Crop Evolution, 2003, 50(3):227-238.
[17]赵鑫闻,彭儒胜,赵继梅,等.分子标记及其在杨树研究中的应用[J].辽宁林业科技,2011(1):48-51.
[18]符毓秦,刘玉媛,李均安,等.美洲黑杨杂种无性系—陕林3、4号杨的选育[J].陕西林业科技,1990, 3(13):1-9.FU Y Q, LIU Y Y, LI J A, et al.Cottonwood hybrided clone-selection of Shaanlin No.3 and No.4[J]. Shaanxi Forest Science and Technology, 1990, 3(13):1-9.

相似文献/References:

[1]周永学,樊军锋,蔺林田,等.美洲黑杨×青杨杂种无性系引种育苗试验[J].西北林学院学报,2004,19(01):58.
 Nursery Trial of the Introduction of Populus deltiodes ×P.cathayana Clone[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2004,19(02):58.
[2]高爱琴,余仲东,王建国.美洲黑杨无性系对杨叶锈病的抗性研究[J].西北林学院学报,2004,19(04):100.
 The Rust Resistance of Populus deltoids Cultivars to Melampsora larici-populina[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2004,19(02):100.
[3]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
 LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(02):21.
[4]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
 ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(02):38.
[5]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
 YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(02):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[6]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
 JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(02):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[7]牛晓霆,王逢瑚,曹新民. 明清家具匠师原木下料的工艺原则及措施[J].西北林学院学报,2013,28(01):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
 NIU Xiao-ting,WANG Feng-hu,CAO Xin-min. Techniques and Principles of Log Cuttings of Furniture Makers in Ming and Qing Dynasties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(02):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
[8]吕金阳,覃卓凯,舒辉,等. 银杉木材构造美学价值[J].西北林学院学报,2013,28(01):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
 LV Jin-yang,QIN Zhuo-kai,SHU Hui,et al. Esthetical Values in Wood Structure of Cathaya argyrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(02):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
[9]杨艳红,张海敏,孙景荣. 基于传统文化视角的现代家具设计探析[J].西北林学院学报,2013,28(01):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
 YANG Yan-hong,ZHANG Hai-min,SUN Jing-rong. Study of Modern Furniture Design Based on Traditional Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(02):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
[10]黄圣游,叶喜. 新中式家具“中式”传统的界定[J].西北林学院学报,2013,28(01):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
 HUANG Sheng-You,YE Xi. Definition of Traditional Element in New Chinese Style Furniture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(02):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
[11]李晓东,樊军锋*,邱兴,等. 美洲黑杨×青杨派杂种无性系苗期抗寒性的鉴定与筛选[J].西北林学院学报,2015,30(02):100.[doi:doi:10.3969/j.issn.1001-7461.2015.02.17]
 LI Xiao-dong,FAN Jun-feng*,QIU Xing,et al. Identification and Selection on the Cold Tolerance in the Hybrids of Populus deltoids×Section tacamahaca[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(02):100.[doi:doi:10.3969/j.issn.1001-7461.2015.02.17]
[12]陈慧玲,张新叶*,黄国伟,等. 美洲黑杨杂交无性系苗期性状联合选择[J].西北林学院学报,2018,33(2):88.[doi:10.3969/j.issn.1001-7461.2018.02.14]
 CHEN Hui-ling,ZHANG Xin-ye*,HUANG Guo-wei,et al. Combined Selection of Hybrid Clones of Populus deltoids at the Seedling Stage[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2018,33(02):88.[doi:10.3969/j.issn.1001-7461.2018.02.14]
[13]郑涛,樊军锋*,高建社,等. 美洲黑杨无性系抗旱性综合评价研究[J].西北林学院学报,2019,34(2):98.[doi:10.3969/j.issn.1001-7461.2019.02.15]
 ZHENG Tao,FAN Jun-feng*,GAO Jian-she,et al. Comprehensive Judgment on Drought-resistance of Populus deltoides[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2019,34(02):98.[doi:10.3969/j.issn.1001-7461.2019.02.15]
[14]田星,张锦梅*,樊军锋. 11种美洲黑杨无性系抗旱性评价[J].西北林学院学报,2019,34(5):79.[doi:10.3969/j.issn.1001-7461.2019.05.12]
 TIAN Xing,ZHANG Jin-mei*,FAN Jun-feng. Drought Resistance Evaluation of 11 Populus deltoides Clones[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2019,34(02):79.[doi:10.3969/j.issn.1001-7461.2019.05.12]

备注/Memo

备注/Memo:
 收稿日期:2013-05-14 修回日期:2013-10-08
基金项目:国家“十二五”科技支撑专题(2012BAD19B0801)。
作者简介:郭娟,女,硕士研究生,研究方向:林木遗传育种、林业生物技术。E-mail:guojuan1323344@163.com
*通信作者:樊军锋,男,研究员,研究方向:林木遗传育种。E-mail:fanjf28@163.com
更新日期/Last Update: