[1]成冰,张京芳*,马正强,等. 酿酒白葡萄品种的SSR分析与鉴定[J].西北林学院学报,2014,29(02):103-106.[doi:doi:10.3969/j.issn.1001-7461.2014.02.18]
 CHENG Bing,ZHANG Jing-fang*,MA Zheng-qiang,et al. SSR Analysis and Identification of Grape Cultivars for Making White Wine[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(02):103-106.[doi:doi:10.3969/j.issn.1001-7461.2014.02.18]
点击复制

 酿酒白葡萄品种的SSR分析与鉴定()
分享到:

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年02期
页码:
103-106
栏目:
出版日期:
2014-03-30

文章信息/Info

Title:
 SSR Analysis and Identification of Grape Cultivars for Making White Wine
文章编号:
1001-7461(2014)-02-0103-04
作者:
 成冰张京芳* 马正强王月晖张贝贝
 (西北农林科技大学 林学院, 陕西 杨陵 712100)
Author(s):
 CHENG Bing ZHANG Jing-fang* MA Zheng-qiang WANG Yue-hui ZHANG Bei-bei
 (College of Forestry, Northwest A&F University, Yangling , Shaanxi 712100, China)
关键词:
 葡萄酒葡萄品种SSR
Keywords:
 wine grape SSR
分类号:
S663.1
DOI:
doi:10.3969/j.issn.1001-7461.2014.02.18
文献标志码:
A
摘要:
 利用筛选出的14对引物对13个酿酒白葡萄品种进行SSR分析与鉴定,共扩增出312个条带,多态性条带共283个(多态性条带率为90.71%),特异性条带共78个(特异性条带率为25.00%);13个品种均有特征性引物,二号白香和雷司令有5个特征性引物;VrZAG79条带总数、多态性条带和特异性条带均为最多(是7个品种的特征性引物),且所有品种的遗传差异性较大;14对引物的鉴别效率为15.38%(VVMD5)~100%(VrZAG62,VVIb66),即VrZAG62,VVIb66可分别鉴定该13个酿酒白葡萄品种且每个引物的鉴别效率差异很大。
Abstract:
 Fourteen SSR primers screened were adopted to identify 13 grape cultivars used to make white wine. Three hundreds and twelve bands were altogether obtained from the amplification, including 283 polymorphic bands (90.71%) and 78 specific bands (25.00%). All the 13 cultivars maintained specific primers. For the cultivars Yarly Madeleine and Riesling, there were five specific primers each. Among 14 primers, VrZAG79, which was the specific primer for seven cultivars, owned more total bands, polymorphic bands and specific bands, indicating large difference among different cultivars. The identification efficiency of 13 cultivars ranged between 15.38%(VVMD5)~100% (VrZAG62, VVIb66). Large difference was shown among different cultivars and two primers (VrZAG62, VVIb66) could identify all the cultivars respectively.

参考文献/References:

 [1]苏占胜. 宁夏酿酒葡萄产量与气象条件的关系[J]. 干旱气象,2012, 30(1):59-65.SU Z S. Relationship between wine grapes yield and weather conditionsin Ningxia[J]. Journal of Arid Meteorology,2012,30(1):59-65. (in Chinese)
[2]张淑静. 葡萄SSR反应体系的建立及遗传多样性分析[D]. 河北:河北农业大学,2008.ZHANG S J. Establishment of SSR reaction system and analysis of genetic diversity of grape cultivars[D]. Hebei: Agricultural University of Hebei, 2008. (in Chinese)
[3]罗国光. 酿酒葡萄产量与质量的关系及其调控[J]. 中国果树,1999 (2): 47-48.LUO G G. Relationship and regulation of grape yield and quality[J]. China Fruits,1999(2):47-48. (in Chinese)

[4]王姣,刘崇怀,樊秀彩,等. 葡萄种类和品种鉴定技术研究进展[J]. 植物遗传资源学报,2008,9(3): 401-405.WANG J, LIU C H, FAN X C, et al.Progress on identification technique of grape species and cuitivars[J]. Journal of Plant Genetic Resource,2008,9(3): 401-405. (in Chinese)
[5]艾呈祥,秦志华,陶吉寒,等. 32个柿主栽品种SSR图谱构建及遗传变异分析[J]. 西北植物学报,2011, 31(11): 2185-2191.AI C X, QIN Z H, TAO J H, et al.SSR Fingerprints and genetic variations of the 32 persimmon maJor cultivars[J]. Acta Botanica Boreali-Occidentalia Sinica, 2011,31(11):2185-2191. (in Chinese)
[6]冷翔鹏,刘崇怀,房经贵,等. 巨峰葡萄系谱的SSR与RAPD分析[J]. 西北植物学报,2011, 31(8): 1560-1566.LENG X P, LIU C H, FANG J G, et al.Analysis of pedigree of dyoho grape vine series with SSR and RAPD [J]. Acta Botanica Boreali-Occidentalia Sinica,2011,31(8):1560-1566. (in Chinese)
[7]刘春英,樊军锋,高建社,等. 杨树新杂种的SSR分析及鉴定[J]. 西北林学院学报2013, 28 (2): 70-73.LIU C Y, FAN J F, GAO J S, et al.Simple sequence repeat analysis and identification of new populus hybrid[J]. Journal of Northwest Forestry University, 2013, 28 (2): 70-73. (in Chinese)
[8]陈艺,郭军战,李辉,等.15个果桑无性系指纹图谱的构建[J].西北林学院学报,2011,26 (6): 62-65.CHEN Y, GUO J Z, LI H, et al.Fingerprints construction of 15 fruit mulberry (Morus spp.) clones[J]. Journal of Northwest Forestry University, 2011,26(6):62-65. (in Chinese)
[9]GORYSLAVETS S, RISOVANNA V, BACILIERI R et al.A parentage study of closely related ukrainian wine grape varieties using microsatellite markers[J]. Cytology and Genetics, 2010,44(2):95-102.
[10]JAHNKE G, MJER J, VARGA P, et al. Analysis of clones of pinots grown in hungary by SSR markers[J]. Scientia Horticulturae,2011,129:32-37.
[11]LORENZIS G D, IMAZIO S, BIAGINI B, et al.Pedigree reconstruction of the Italian grapevine aglianico (Vitis vinifera L.) from campania[J]. Molecular Biotechnology, 2013,54(2):634-642.
[12]BERT P F, BORDENAVE L, DONNART M,et al.Mapping genetic loci for tolerance to lime-induced iron deficiency chlorosis in grapevine rootstocks (Vitis sp.) [J]. Theoretical and Applied Genetics, 2013,126(2):451-473. 
[13]AGAR G, YILDIRIM N, ERCISLI S, et al.Determination of genetic diversity of Vitis vinifera cv. kabarcik populations from the coruh Valley using SSR markers[J]. Biochemical Genetics,
2012,50(5/6):476-483.
[14]KARATASA H, DEEIRMENCIB D, VELASCOC R, et al.Microsatellite fingerprinting of homonymous grapevine (Vitis vinifera L.) varieties in neighboring regions of South-East Turkey [J].Scientia Horticulturae,2007,114(3):164-169.
[15]GRANDO M S, BELLIN D, EDWARDS K J,et al.Molecular linkage maps of Vitis vinifera L.and Vitis riparia Mchx.[J]. Theoretical and Applied Genetics, 2003,106(7):1213-1224.
[16]CHACN J L, GARCA E, MARTNEZ J, et al.Comparison of aromatic composition of an endangered variety (’Albilla Dorada’) with other recognized aromatic varieties[J].
Vitis,2012,51(1):15-17.
[17]郝宇,张淑静,张世红,等. 葡萄品种资源的SSR鉴定及遗传多样性分析[J]. 河北农业大学学报,2010,33(1):54-59.HAO Y, ZHANG S J, ZHANG S H, et al.SSR identi fication and analysis of genetic diversity of grape cultivars[J]. Journal of Agricultural University of Hebei, 2010,33(1):54-59. (in Chinese).

相似文献/References:

[1]房玉林,张昂,宋士任,等.3个地区赤霞珠干红葡萄酒香气成分研究[J].西北林学院学报,2007,22(06):114.
 FANG Yulin,ZHANG Ang,SONG Shiren,et al.Aroma Components in ‘Cabernet Sauvignon’ Wines from Three Areas Different Production Areas[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(02):114.
[2]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
 LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(02):21.
[3]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
 ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(02):38.
[4]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
 YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(02):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[5]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
 JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(02):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[6]牛晓霆,王逢瑚,曹新民. 明清家具匠师原木下料的工艺原则及措施[J].西北林学院学报,2013,28(01):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
 NIU Xiao-ting,WANG Feng-hu,CAO Xin-min. Techniques and Principles of Log Cuttings of Furniture Makers in Ming and Qing Dynasties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(02):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
[7]吕金阳,覃卓凯,舒辉,等. 银杉木材构造美学价值[J].西北林学院学报,2013,28(01):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
 LV Jin-yang,QIN Zhuo-kai,SHU Hui,et al. Esthetical Values in Wood Structure of Cathaya argyrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(02):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
[8]杨艳红,张海敏,孙景荣. 基于传统文化视角的现代家具设计探析[J].西北林学院学报,2013,28(01):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
 YANG Yan-hong,ZHANG Hai-min,SUN Jing-rong. Study of Modern Furniture Design Based on Traditional Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(02):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
[9]黄圣游,叶喜. 新中式家具“中式”传统的界定[J].西北林学院学报,2013,28(01):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
 HUANG Sheng-You,YE Xi. Definition of Traditional Element in New Chinese Style Furniture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(02):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
[10]韩维生,赵明磊,王宏斌. 基于阈限理论的设计尺度体系[J].西北林学院学报,2013,28(01):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
 HAN Wei-sheng,ZHAO Ming-lei,WANG Hong-bin. Design Scale System Based on Threshold Theory[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(02):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]

备注/Memo

备注/Memo:
 收稿日期:2013-06-02 修回日期:2013-09-23
基金项目:公益性行业(农业)科研专项(200903043)。
作者简介:成冰,女,硕士研究生,研究方向:果蔬深加工及综合利用。E-mail:blithe0404@hotmail.com
*通信作者:张京芳,女,教授,理学博士,研究方向:食品资源营养及功能活性物质。E-mail:z_jf008@163.com
更新日期/Last Update: