[1]易允喻,马希汉,赵忠,等. 苦杏壳木醋液最小抑菌浓度及其抑菌活性的稳定性[J].西北林学院学报,2014,29(06):127-131.[doi:doi:10.3969/j.issn.1001-7461.2014.06.24]
　YI Yun-yu,MA Xi-han,ZHAO Zhong,et al. Minimal Inhibitory Concentration and Activity Stability of Bitter Almond Shell Pyroligneous Acid to Bacteria[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(06):127-131.[doi:doi:10.3969/j.issn.1001-7461.2014.06.24]
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 苦杏壳木醋液最小抑菌浓度及其抑菌活性的稳定性()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年06期
页码:
127-131
栏目:
出版日期:
2014-11-30

文章信息/Info

Title:
 Minimal Inhibitory Concentration and Activity Stability of Bitter Almond Shell Pyroligneous Acid to Bacteria
文章编号:
1001-7461(2014)06-0127-05
作者:
 易允喻1; 马希汉2; 赵忠1; 尉芹1*; 李宏伟1
 （1.西北农林科技大学 林学院，陕西 杨陵 712100；2. 西北农林科技大学 理学院，陕西 杨陵 712100）
Author(s):
 YI Yun-yu1;  MA Xi-han2;  ZHAO Zhong1;  WEI Qin1*;  LI Hong-wei1
 (1.College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China; 2. College of Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China)
关键词:
 ; 苦杏壳木醋液; D值法; 最小抑菌浓度(MIC); 抑菌; 稳定性
Keywords:
 ; bitter almond shell pyroligneous acid;  method of decimal reduction time;  MIC;  antibacterial activity;  stability
分类号:
S731
DOI:
doi:10.3969/j.issn.1001-7461.2014.06.24
文献标志码:
A
摘要:
 采用金黄色葡萄球菌(Staphylococcus aureus)、大肠杆菌(Escherichia coli)作为指示菌，由D值计算法得到了对苦杏壳木醋液在不同浓度时的存活曲线及死亡曲线，从而精确求得最小抑菌浓度(MIC)：木醋液对金黄色葡萄球菌和大肠杆菌的MIC分别为0.44%和0.46%，可见木醋液对2种代表性细菌有较显著的抑菌效果；采用滤纸片法，对木醋液抑菌稳定性测试结果表明，苦杏壳木醋液在温度、pH值、紫外光、活性碳吸附及微波加热等不同处理下，抑菌效果基本不变，具有良好的抑菌稳定性。
Abstract:
 Staphylococcus aureus and Escherichia coli were used as indicator bacteria to investigate the minimal inhibitory concentration (MIC) of pyroligneous acid made from bitter almond shell. The procedures included to obtain the survival and mortal curves under different concentrations of the acid by decimal reduction time method (D value method), and then to get MIC. The MIC of pyroligneous acid to S. aureus and E. coli were 0.44% and 0.46%, respectively, indicating the significant inhibitory effects of the acid. The inhibition stability of the acid to temperature, acid, alkali, ultraviolet, activated carbon, and microwave were also studied by filter paper method. The results demonstrated that the acid almost kept the same level of the inhibition to bacteria under different treatments, showing its satisfactory antibacterial stability.

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备注/Memo

备注/Memo:
 收稿日期：2013-12-16修回日期：2014-04-29
基金项目：陕西省2010年“13115”科技创新工程重大科技专项计划项目（2010ZDKG-05）。
作者简介：易允喻，女，在读硕士，研究方向：树木提取物化学与工程。E-mail:altayiyunyu@gmail.com
*通信作者：尉芹，女，教授，博导，研究方向：植物资源化学与生物活性物质。E-mail:ma_wei_qin@aliyun.com.cn
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