[1]赵莘,段丽娟,高健,等. Folin-Ciocalteu法测定大熊猫主食竹中多酚含量[J].西北林学院学报,2014,29(06):212-216.[doi:doi:10.3969/j.issn.1001-7461.2014.06.40]
　ZHAO Shen,DUAN Li-juan,Gao Jian,et al. Determination of the Contents of Polyphenols in Fargesia denudata, the Main Food of Giant Penda by Folin-Ciocalteu Colorimetric Method[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(06):212-216.[doi:doi:10.3969/j.issn.1001-7461.2014.06.40]
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 Folin-Ciocalteu法测定大熊猫主食竹中多酚含量()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年06期
页码:
212-216
栏目:
出版日期:
2014-11-30

文章信息/Info

Title:
 Determination of the Contents of Polyphenols in Fargesia denudata, the Main Food of Giant Penda by Folin-Ciocalteu Colorimetric Method
文章编号:
1001-7461(2014)06-0212-05
作者:
 赵莘; 段丽娟; 高健; 郑丽丽; 李俊清*
 （北京林业大学 省部共建森林培育与保护教育部重点实验室，北京 100083）
Author(s):
 ZHAO Shen;  DUAN Li-juan;  Gao Jian;  ZHENG Li-li;  LI Jun-qing*
 (The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China)
关键词:
 ; 缺苞箭竹; Folin-Ciocalteu法; 总酚
Keywords:
 ; Fargesia denudata;  Folin-Ciocalteu;  ployphenol
分类号:
S795.2
DOI:
doi:10.3969/j.issn.1001-7461.2014.06.40
文献标志码:
A
摘要:
 为建立 Folin-Ciocalteu法测定大熊猫主食竹缺苞箭竹(Fargesia denudata)总酚酸含量的方法。以没食子酸为对照品，研究了Folin-Ciocalteu法测定缺苞箭竹中酚酸类物质含量的适宜条件。结果表明，适宜的比色条件为Folin-Ciocalteu试剂与 10% Na2CO3体积比为1∶6，Folin-Ciocalteu试剂3 mL，显色温度25℃，反应时间2 h，检测波长780 nm。浓度为20～100 μg·mL-1时与其吸光值呈良好的线性关系，测定方法的平均回收率为99.4%。可见该法有较好的准确度和重现性。
Abstract:
 Aims: to establish a quantitative method of measuring total phenols in Fargesia denudata, the main food of giant panda. Methods: with gallic acid as reference substance to study the suitable conditions of the measurement by Folin-Ciocalteu method. Results: the optimum conditions for determining total polyphenols were as follows: the volume ratio of Folin-Ciocalteu reagent to 10% Na2CO3: 1∶6, 3 mL of Folin -Ciocalteu reagent incubated at 25℃for 2 h, followed by detection at 780 nm. Good linear relationship was found in the concentration of 20-100 μg·mL-1, the average recovery was 99.4%.

参考文献/References:

 [1]ZHANG Z J, WEI F W, LI M, et al. Microhabitat separation during winter among sympatric giant pandas, red pandas, and tufted deer: the effects of diet, body size, and energy metabolism[J]. Canadian Journal of Zoology, 2004, 82(9):1451-1457.
[2]胡锦矗.大熊猫的食性研究[J].西华师范大学学报：自然科学版,1981（3）:17-22.
HU J C. The giant panda diet research[J]. Journal of China West Normal University:Natural Sciences, 1981（3）:17-22.(in Chinese)
[3]CAMPBELL J J, QIN Z S. Interaction of giant pandas, bamboos, people[J]. Journal of the American Bamboo Society, 1984,4(1/2):1-35.
[4]SCHALLER G B, TENG Q T, JOHNSON K G, et al. Feeding ecology of giant panda and asiatic black bear in the Tangjiahe reserve, China. in: Gittleman. Carnivore Behavior, Ecology and Evolution[C]. Ithaca, New York: Cornell University Press, 1987.
[5]石碧，狄莹.植物多酚[M]. 北京:科学出版社, 2000:19-20.
[6]PAIGE K N. Overcompensation in response to mammalian herbivory: from mutualistic to antagonistic interactions[J]. Ecology,1992, 73(6): 2076-2085.
[7]STRAUSS SY, SIEMENS D H, DECHER M B, et al. Ecological costs of plant resistance to herbivores in the currency of pollination[J]. Evolution, 1999, 53: 1105-1113.
[8]程春龙,李俊清.植物多酚的定量分析方法和生态作用研究进展[J].应用生态学报,2006,17(12):2475-2460.
CHENG C L, LI J Q. Research advances in ecological significance and quantification of plant poly phenols[J]. Chinese Journal of Applied Ecology, 200617(12):2475-2460. (in Chinese)
[9]赵晓红,刘广平,马泽芳.竹子中单宁含量的测定及其对大熊猫采食量的影响[J].东北林业大学学报,2001,29(2):67-71.
ZHAO X H, LIU G P, MA Z F. Determination of the tannin in bamboo and its effect on the panda[J]. Journal of Northeast Forestry University, 2001,29(2):67-71. (in Chinese)
[10]何东阳.大熊猫取食竹选择、消化率及营养和能量对策的研究[D]. 北京:北京林业大学,2010.
[11]唐平,周昂,李操,等.冶勒自然保护区大熊猫摄食行为及营养初探[J].四川师范学院学报:自然科学版,1997,18(1)：1-4.
TANG P, ZHOU A, LI C, et al. An investigation of the foraging behaviour and nutritious condition of the giant panda in Yele Natural Reserve[J]. Journal of Sichuan Teachers College:Natural Sciences, 1997,18(1):1-4. (in Chinese)
[12]VAN HOVEN L. Tannins and digestibility in Greater Kudu[J]. Canadian Journal of Plant Science,1984,64:177-198.
[13]HASLAM E, LILLEY T H. Natural astringency in foodstuffs-a molecular interpretation[J]. CRC Critical Reviews in Food Science and Nutrition.1988, 27(1): 1-40.
[14]王爱民 邹兴淮 魏广强.大熊猫常食竹类的“双效”作用及保护对策[J], 林业科学,2006,42(5):94-97.
WANG A M, ZOU X H, WEI G Q. The dual effect of the giant panda edible bamboo and prevetion counter measures[J]. Scientia Silvae Sinicae,2006,42(5):94-97. (in Chinese)
[15]杨道贵,宿以明,向永国,等.王朗引种区大熊猫主食竹生长发育规律的研究[J].竹子研究汇刊1992,11(4):26-36.
YANG D G, SU Y M, XIANG Y G, et al. Wanglang national giant panda staple food bamboo introduction research on the laws of the growth and development[J]. Journal of Bamboo Research,1992,11(4):26-36. (in Chinese)
[16]牟克华, 史立新. 大熊猫主食竹—冷箭竹生物学特性的研究[J].竹子研究汇刊,1991,10(4): 24-32.
MO K H,SHI L X. The giant panda staple food bamboo-cold arrow bamboos biological characteristics research [J]. Journal of Bamboo Research, 1991,10(4): 24-32. (in Chinese)
[17]周世强,黄金燕,王鹏彦,等.大熊猫野化培训圈主食竹种生长发育特性及生物量结构调查[J]. 竹子研究汇刊,2004,23(2):15-20
ZHOU S Q, HUANG J Y, WANG P Y, et al. A study on the feature of bamboo growth and biomass structurein giant panda’s field training area[J]. Journal of Bamboo Research, 2004,23(2): 15-20. (in Chinese)
[18]罗定泽,赵佐成,王季勋.四川王朗自然保护区大熊猫主食竹—缺苞箭竹(Fargesia denudat)不同发育时期酯酶和a-淀粉酶同工酶的研究[J].武汉植物学研究,1989,7(3):263-267. 
LUO D Z, ZHAO Z C, WANG J X. Sichuan Wanglang Nature Reserve for giant pandas staple food bamboo- lack of Fargesia denudate in different development period of esterase and a-amylase isozyme research[J]. Journal of Wuhan Botanical Research,1989,7(3):263-267. (in Chinese)
[19]周材权,胡锦矗,任丽平.马边大风顶自然保护区大熊猫二主食竹微量元素的研究[J].四川师范学院学报：自然科学版,1997,18(1): 5-9.
ZHOU C Q, HU J C, REN L P. Study of the trace elements in two species of bamboos,the main components of the diet of the gaint panda in Mabiandafengding Natural Reserve[J]. Journal of Sichuan Teachers College:Natural Science, 1997,18(1): 5-9. (in Chinese)
[20]闵红,范智超,张志琪,七叶鬼灯檠中多酚类化合物提取工艺研究与含量测定[J].西北林学院学报,2007,22(3):138-140.
MIN H, FAN Z C, ZHANG Z Q, Extractionand determination of total polyphenol from the root of Rodgersia aesculifolia[J], Journal of Northwest Forestry University,2007,22(3):138-140. (in Chinese)
[21]解蕊,亚高山不同针叶林冠下大熊猫主食竹的克隆生长[D]. 北京:北京林业大学,2009.
[22]贾炅,武吉华.四川王郎自然保护区大熊猫主食竹天然更新[J].北京师范大学学报:自然科学版,1991,27(2);250-256
JIA J, WU J H. Sichuan Wanglang Nature Reserves pandas staple food bamboo natural regeneration [J].Journal of Beijing Normal University:Natural Sciences,1991,27(2):250-256. (in Chinese)
[23]TSIMIDOU M, PAPADOPOULOS G, BOSKOU D. Phenolic compounds and stability of virgin olive oil Part I[J]. Food Chemistry, 1992,45:141-144.
[24]吴萼,徐宁,温美娟.磷钼酸—磷钨酸盐比色法测定土壤中总酚酸含量[J].环境化学,2000,19(1):67-72.
WU E, XU N, WEN M J. The measurement of total phenolic acids in soil by phosphomolybdic-phosphotungstic acid phenol reagent colorimetry[J]. Environmental Chemistry, 2000,19(1): 67-72. (in Chinese)
[25]SINGLETON V L, ROSSI J A. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents[J]. American Journal of Enology and Viticulture,1965,16: 144-158.

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备注/Memo

备注/Memo:
 收稿日期：2014-02-25修回日期：2014-03-19
基金项目：林业公益性行业科研专项（201404422）。
作者简介：赵莘，女，博士，研究方向：生物多样性保护。E-mail：zhaoshen1993@163.com
*通信作者：李俊清，男，教授，研究方向：恢复生态学。E-mail：Lijq@bjfu.edu.cn
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