[1]郭素娟,孙小兵,秦天天,等. 板栗成熟胚再生体系的建立与优化[J].西北林学院学报,2015,30(03):89-93.[doi:doi:10.3969/j.issn.1001-7461.2015.03.16]
　GUO Su-juan,SUN Xiao-bing,QIN Tian-tian,et al. Establishment and Optimization of in vitro Regeneration System of Mature Embryo of Castanea mollissima[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(03):89-93.[doi:doi:10.3969/j.issn.1001-7461.2015.03.16]
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 板栗成熟胚再生体系的建立与优化()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第30卷
期数:
2015年03期
页码:
89-93
栏目:
出版日期:
2015-05-31

文章信息/Info

Title:
 Establishment and Optimization of in vitro Regeneration System of Mature Embryo of Castanea mollissima
文章编号:
1001-7461(2015)03-0089-05
作者:
 郭素娟; 孙小兵; 秦天天; 刘正民
 （省部共建森林培育保护与利用教育部重点实验室，北京林业大学, 北京10083）
Author(s):
 GUO Su-juan;  SUN Xiao-bing;  QIN Tian-tian;  LIU Zheng-min
 (Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing 100083, China)
关键词:
 ; 板栗‘燕山早丰’; 种胚; 组织培养
Keywords:
 ; ‘Yanshanzaofeng’;  embryos;  tissue culture
分类号:
S722.37
DOI:
doi:10.3969/j.issn.1001-7461.2015.03.16
文献标志码:
A
摘要:
 研究了外植体消毒方法、植物生长调节剂组合对胚萌发和生长的影响。以迁西板栗主栽品种‘燕山早丰’成熟胚为试验材料，采用L9(34)试验设计对增殖培养基进行筛选。结果表明，二次消毒处理可以有效抑制污染，污染率最低为16.67%。胚萌发和生长最佳基本培养基为WPM+6-BA 1.0 mg·L-1，萌发率最高达到95.00%，胚萌发苗最高为59.65 mm。不定芽诱导的最适培养基为：MS+6-BA 1.0 mg·L-1+IBA 0.1 mg·L-1。芽诱导率最高为92.50%。最适增殖培养基为GD+ZT 2.0 mg·L-1+NAA 0.1 mg·L-1，增殖系数最大为3.91。
Abstract:
 Effects of sterilization method and the combination of plant growth regulators on the embryo germination and growth of Castanea mollissima were examined by taking the cultivar ‘Yanshanzaofeng’ from Qianxi County as test material, and using L9(34) orthogonal experimental design. It was found that the two-time sterilization treatment could effectively inhibit the contamination, and the lowest rate of contamination was 16.67%. The optimum medium for embryo germination and its growth was WPM+6-BA 1.0 mg·L-1, by which the highest germination rate reached 95.00%, seedlings from the embryo were as high as 59.65 mm. The optimum medium for adventitious buds was MS+6-BA 1.0 mg·L-1+IBA 0.1 mg·L-1, by which the highest induction rate of adventitious bud was 92.50%. The optimum rearing medium was GD+ZT 2.0 mg·L-1+NAA 0.1 mg·L-1, the maximum proliferation factor was 3.91.

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备注/Memo

备注/Memo:
 收稿日期：2014-05-28 修回日期：2014-12-18
基金项目：国家“十二五”科技支撑计划专项 (2013BAD14B0402)。
作者简介：郭素娟，女，教授，博士生导师，研究方向：森林培育。E-mail：gwangzs@263.net
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