[1]冯连荣,宋立志,张妍,等. 根癌农杆菌介导杨树遗传转化的影响因素[J].西北林学院学报,2015,30(03):120-126.[doi:doi:10.3969/j.issn.1001-7461.2015.03.21]
 FENG Lian-rong,SONG Li-zhi,ZHANG Yan,et al. Influence Factors on Agrobacterium mediated Transformation Efficiency of Populus[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(03):120-126.[doi:doi:10.3969/j.issn.1001-7461.2015.03.21]
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 根癌农杆菌介导杨树遗传转化的影响因素()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第30卷
期数:
2015年03期
页码:
120-126
栏目:
出版日期:
2015-05-31

文章信息/Info

Title:
 Influence Factors on Agrobacterium mediated Transformation Efficiency of Populus
文章编号:
1001-7461(2015)03-0120-07
作者:
 冯连荣宋立志张妍彭儒胜赵大根
 (辽宁省杨树研究所,辽宁 盖州 115200)
Author(s):
 FENG Lian-rong SONG Li-zhi ZHANG Yan PENG Ru-sheng ZHAO Da-gen
 (Liaoning Provincial Institute of Poplar, Gaizhou, Liaoning 115200, China)
关键词:
 根癌农杆菌杨树遗传转化影响因素
Keywords:
Agrobacterium Populus transformation influence factor
分类号:
S792.11
DOI:
doi:10.3969/j.issn.1001-7461.2015.03.21
文献标志码:
A
摘要:
 针对根癌农杆菌介导杨树遗传转化过程中杨树受体系统的建立、质粒转化载体的构建、目的基因转化和转化体选择4部分影响因子进行综述。影响因子包括杨树基因型、外植体类型等内部因素,培养基、培养条件、vir基因诱导物、抗生素等外部因素。总结了在实际操作中应该注意的问题,为更好地利用根癌农杆菌介导法开展杨树遗传转化,提高转化效率提供借鉴和参考。
Abstract:
Agrobacterium mediated transformation is the main method used in the study of transgenic Populus. Influence factors on Agrobacterium mediated transformation efficiency of Populus were reviewed from 4 aspects, including regeneration system, construction of transformation vector, transformation target gene and selection of transformant. The factors influencing Agrobacterium mediated transformation efficiency of Populus were examined, including internal factor (Populus genotypes and explants types), and external factor (medium, culture condition, inducer of vir gene and antibiotic). Problems in the practice application were analyzed to provide guidance for improving Agrobacterium mediated transformation efficiency of Populus.

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备注/Memo

备注/Memo:
 收稿日期:2014-09-01 修回日期:2014-12-04
基金项目:辽宁省科学公益事业研究基金(2014002016)。
作者简介:冯连荣,女,硕士,工程师,研究方向:杨树转基因育种及病虫害防治。E-mail:fenglianrong@163.com
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