[1]酒立君,易盼盼,王飞*,等. 不同浓度水杨酸处理对蝴蝶兰组培褐变的影响[J].西北林学院学报,2015,30(03):127-131.[doi:doi:10.3969/j.issn.1001-7461.2015.03.22]
　JIU Li-jun,YI Pan-pan,WANG Fei*,et al. Effects of Salicylic Acid Treatment with Different Concentrations on Tissue Browning of Phalaenopsis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(03):127-131.[doi:doi:10.3969/j.issn.1001-7461.2015.03.22]
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 不同浓度水杨酸处理对蝴蝶兰组培褐变的影响()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第30卷
期数:
2015年03期
页码:
127-131
栏目:
出版日期:
2015-05-31

文章信息/Info

Title:
 Effects of Salicylic Acid Treatment with Different Concentrations on Tissue Browning of Phalaenopsis
文章编号:
1001-7461(2015)03-0127-05
作者:
 酒立君1; 易盼盼2; 王飞2*; 张庆雨1; 杨凤美1; 吴楠1
 （1.西北农林科技大学 林学院，陕西 杨陵 712100；2.西北农林科技大学 园艺学院，陕西 杨陵 712100）
Author(s):
 JIU Li-jun1;  YI Pan-pan2;  WANG Fei2*;  ZHANG Qing-yu1;  YANG Feng-mei1;  WU Nan1
 (1. College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China; 2. College of Horticulture, Nurthwest A&F University, Yangling, Shaanxi 712100, China)
关键词:
 ; 蝴蝶兰; 水杨酸; 组培褐变
Keywords:
Phalaenopsis;  salicylic acid;  tissue browning
分类号:
S722.89
DOI:
doi:10.3969/j.issn.1001-7461.2015.03.22
文献标志码:
A
摘要:
 以蝴蝶兰品种‘内山姑娘’的组培苗叶片为试验材料，将不同浓度的水杨酸（SA）添加于培养基中并观察叶片的褐变情况，筛选出SA的安全使用浓度为0~0.2 mmol·L-1。测定0.05、0.1和0.2 mmol·L-1的SA处理对叶片褐变指数、总酚（TP）含量、苯丙氨酸解氨酶（PAL）、多酚氧化酶（PPO）、过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶（CAT）和抗坏血酸过氧化物酶(APX)活性的影响。结果表明，SA处理可降低蝴蝶兰叶片褐变过程中的褐变指数、总酚含量和PAL活性，提高SOD、POD和CAT活性。0.1 mmol·L-1的SA处理抑制外植体褐变的效果较好。
Abstract:
 A Phalaenopsis cultivar ’Neyshan Guniang’ was cultured on the medium with different concentrations (0.05, 0.1 mmol·L-1 and 0.2 mmol·L-1) of salicylic acid (SA), the browning situation of the leaves cultured were observed. The browning index, the activities of TP, PAL, PPO, POD, SOD, CAT and APX were measured. The results showed that the safe dosages of SA use were between 0 to 0.2 mmol·L-1, the SA treatment could decrease browning index，total phenol content and PAL activity, and increase the SOD, POD and CAT activities. Satisfactory results of inhibiting leaf browning were achieved when the concentration of SA was 0.1 mmol·L-1.

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备注/Memo

备注/Memo:
 收稿日期：2014-06-21 修回日期：2014-12-23
基金项目：西安园林观赏花卉示范园建设子项目（NC10031(2)）。
作者简介：酒立君，女，在读硕士，研究方向：花卉生理生态。E-mail：jiu715191795@126.com
*通信作者：王飞，女，教授，研究方向：园艺植物种质资源评价与利用。E-mail：xnwangfei521@126.com
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