[1]王晓林,钟方丽*,薛健飞,等. 微波协同酶法提取刺玫叶总黄酮工艺研究[J].西北林学院学报,2015,30(04):246-250.[doi:doi:10.3969/j.issn.1001-7461.2015.04.40]
　WANG Xiao-lin,ZHONG Fang-li*,XUE Jian-fei,et al. Extraction Technology of Total Flavonoids in the Leaves of Rosa davurica with Microwave Assisted Enzymes Method[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(04):246-250.[doi:doi:10.3969/j.issn.1001-7461.2015.04.40]
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 微波协同酶法提取刺玫叶总黄酮工艺研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第30卷
期数:
2015年04期
页码:
246-250
栏目:
出版日期:
2015-07-31

文章信息/Info

Title:
 Extraction Technology of Total Flavonoids in the Leaves of Rosa davurica with Microwave Assisted Enzymes Method
文章编号:
1001-7461(2015)04-0246-05
作者:
 王晓林; 钟方丽*; 薛健飞; 单雪娜
 (吉林化工学院 化学与制药工程学院，吉林 吉林 132022)
Author(s):
 WANG Xiao-lin;  ZHONG Fang-li*;  XUE Jian-fei;  SHAN Xue-na
 (School of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin, Jilin 132022, China)
关键词:
 ; 刺玫叶; 总黄酮; 酶解; 提取
Keywords:
Rosa davurica leaf;  total flavonoid;  enzymolysis;  extract
分类号:
S793
DOI:
doi:10.3969/j.issn.1001-7461.2015.04.40
文献标志码:
A
摘要:
 采用微波协同酶法提取刺玫叶总黄酮，以刺玫叶总黄酮提取率为主要指标对微波协同酶法提取工艺进行了优化，确定了最佳的工艺条件：提取溶剂为75%乙醇、料液比1∶25，复合酶（纤维素酶∶果胶酶=2∶1），酶解温度55℃、酶解时间120 min、酶解提取2次、酶解体系pH 4.5、微波功率600 W，微波处理时间15 min、微波温度60℃、微波提取2次，在此工艺条件下，刺玫叶总黄酮平均提取率为32.21 mg·g-1。表明微波协同酶法可用于刺玫叶总黄酮的提取。
Abstract:
 Microwave assisted enzymic method was adopted to extract total flavonoids (TF) in the leaves of Rosa davurica. The extraction efficiency of TF was used as examination indicator, while the extraction technology was optimized and determined. The concentration of the ethanol extraction solvent, the ratio between raw material and solvent, the complex enzyme ratio between cellulase and pectase, the temperature, time and times of enzymolysis, the pH value of enzymolysis, the power of microwave generator, extracting time, temperature and extraction times were 75%, 1∶25, 2∶1, 55℃, 120 min, 2 times, 4.5, 600 W, 15 min, 60℃, and 2 times, respectively. The average extraction efficiency of TF was 32.21 mg·g-1 under the optimized extraction conditions, which showed that enzymes method was suitable for extracting TF in the leaves of R. davurica.

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备注/Memo

备注/Memo:
 收稿日期：2014-09-15修回日期：2014-11-03
基金项目：吉林省科技厅计划项目（20110948）。
作者简介：王晓林，男，副教授，硕士，研究方向：天然产物有效成分的提取、纯化及药物的研制与开发。E-mail：wangxiaolin69@eyou.com
*通信作者：钟方丽，女，教授，博士，研究方向：天然产物化学成分的分离与生物活性。E-mail：fanglizhong@sina.com
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