[1]姜福星,杨丽娟,陈其兵*,等. 泸定百合转录组测序与特性分析[J].西北林学院学报,2015,30(05):143-150.[doi:doi:10.3969/j.issn.1001-7461.2015.05.23]
　JIANG Fu-xing,YANG Li-juan,CHEN Qi-bing*,et al. Transcriptome Sequencing and Characteristic Analysis of Lilium sargentiae Wilson[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(05):143-150.[doi:doi:10.3969/j.issn.1001-7461.2015.05.23]
点击复制

 泸定百合转录组测序与特性分析()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第30卷
期数:
2015年05期
页码:
143-150
栏目:
出版日期:
2015-09-30

文章信息/Info

Title:
 Transcriptome Sequencing and Characteristic Analysis of Lilium sargentiae Wilson
文章编号:
1001-7461(2015)05-0143-08
作者:
 姜福星1; 杨丽娟1; 陈其兵2*; 高顺3
 (1.四川农业大学 园林研究所，四川 成都 611130； 2.四川农业大学 风景园林学院，四川 成都 611130；3.四川农业大学 生态林业研究所，四川 成都 611130)
Author(s):
 JIANG Fu-xing1;  YANG Li-juan1;  CHEN Qi-bing2*;  GAO Shun3
 (1. Institute of Garden Research, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; 2. Institute of Landscape and Architecture, Chengdu, Sichuan 611130, China; 3. Institute of Forsetry Ecology, Chengdu, Sichuan 611130, China)
关键词:
 ; 泸定百合; 鳞茎; 转录组测序; 特性分析
Keywords:
Lilium sargentiae;  bulb;  transcriptome sequencing;  characteristic analysis
分类号:
Q943
DOI:
doi:10.3969/j.issn.1001-7461.2015.05.23
文献标志码:
A
摘要:
 泸定百合是非常优良的种质基因资源，鳞茎研究是其科研和生产的关键环节，然而泸定百合鳞茎的分子生物学研究尚属空白。为揭示泸定百合鳞茎的分子机制，应用高通量测序技术对泸定百合的鳞茎进行转录组分析。原始数据经过生物信息学分析后，共获得4.9 G有效数据，43 412条Unigene；所获得的Unigene与NR,GO,COG和KEGG等数据库进行搜索比对后，发现有30 225条和21 531条Unigene分别获得NR和GO注释，14 069条Unigene得到COG注释，GO注释中获得374条Unigene可能作为转录因子参与泸定百合鳞茎的形成与发育；共有10 822条Unigene参与了KEGG代谢途径，其中有571条Unigene注释到次生代谢合成途径，其中66条和23条Unigene分别参与了萜类和生物碱的合成。研究结果为采用生物技术手段提高百合的药用成分的含量及改善其园艺性状积累了基本资料。
Abstract:
Lilium sargentiae is a very excellent germplasm gene resource. Understanding of its bulbs is a key link for the scientific research and production. However, little is known about the molecular biology of the bulbs. To reveal the molecular mechanism of its bulbs, transcriptome analysis was carried out by high-throughput sequencing technology. After bioinformatics analysis, 4.9 G valid data and 43 412 Unigene were obtained from the raw data. After comparing with and NR, GO, COG and KEGG search database，among the obtained Unigene, 30 225 and 21 531 Unigenes were assigned into NR and GO respectively, 14 069 Unigene was assigned into COG, 374 Unigene from GO function classification could take part into formation and development of the bulbs of L. sargentiae as transcription factor;10 822 Unigene was assigned into KEGG pathway, 571 Unigenes was involved in biosynthesis of secondary metabolites, in which 66 and 23 Unigene were involved in terpenoid and alkaloid biosynthesis, respectively.

相似文献/References:

[1]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
　LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(05):21.
[2]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
　ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(05):38.
[3]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
　YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(05):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[4]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
　JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(05):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[5]牛晓霆,王逢瑚,曹新民. 明清家具匠师原木下料的工艺原则及措施[J].西北林学院学报,2013,28(01):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
　NIU Xiao-ting,WANG Feng-hu,CAO Xin-min. Techniques and Principles of Log Cuttings of Furniture Makers in Ming and Qing Dynasties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(05):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
[6]吕金阳,覃卓凯,舒辉,等. 银杉木材构造美学价值[J].西北林学院学报,2013,28(01):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
　LV Jin-yang,QIN Zhuo-kai,SHU Hui,et al. Esthetical Values in Wood Structure of Cathaya argyrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(05):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
[7]杨艳红,张海敏,孙景荣. 基于传统文化视角的现代家具设计探析[J].西北林学院学报,2013,28(01):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
　YANG Yan-hong,ZHANG Hai-min,SUN Jing-rong. Study of Modern Furniture Design Based on Traditional Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(05):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
[8]黄圣游,叶喜. 新中式家具“中式”传统的界定[J].西北林学院学报,2013,28(01):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
　HUANG Sheng-You,YE Xi. Definition of Traditional Element in New Chinese Style Furniture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(05):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
[9]韩维生,赵明磊,王宏斌. 基于阈限理论的设计尺度体系[J].西北林学院学报,2013,28(01):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
　HAN Wei-sheng,ZHAO Ming-lei,WANG Hong-bin. Design Scale System Based on Threshold Theory[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(05):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
[10]宋杰,侯建军,申黎明. 按摩椅按摩头包覆层对按摩舒适性的影响[J].西北林学院学报,2013,28(01):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
　SONG Jie,HOU Jian-jun,SHEN Li-ming. Influence of Massager Coat Layer of Massage Chairs on the Degree of Comfort[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(05):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]

备注/Memo

备注/Memo:
 收稿日期：2014-11-24修回日期：2015-01-28
基金项目：四川农业大学学科建设双支计划（64070105）；2015年四川省教育厅重点项目(035Z1025)。
作者简介：姜福星，男，博士，研究方向：园林植物发育生物学。E-mail:bnjfx@126.com 
*通信作者：陈其兵，男，教授，研究方向：园林植物栽培与育种。E-mail:cqb@sicau.edu.cn
更新日期/Last Update: