[1]杨青青,南小宁,王云果,等. 柏大蚜和松大蚜体内细菌的16S rRNA-RFLP分析[J].西北林学院学报,2015,30(06):168-173.[doi:doi:10.3969/j.issn.1001-7461.2015.06.31]
 YANG Qing-qing,NAN Xiao-ning,WANG Yun-guo,et al. Composition of the Bacteria Associated with Cinara tujafilina and C. pinitabulaeformis Based on 16S rRNA-RFLP Analysis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(06):168-173.[doi:doi:10.3969/j.issn.1001-7461.2015.06.31]
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 柏大蚜和松大蚜体内细菌的16S rRNA-RFLP分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第30卷
期数:
2015年06期
页码:
168-173
栏目:
出版日期:
2015-11-30

文章信息/Info

Title:
 Composition of the Bacteria Associated with Cinara tujafilina and C. pinitabulaeformis Based on 16S rRNA-RFLP Analysis
文章编号:
1001-7461(2015)06-0168-06
作者:
 杨青青南小宁王云果郭新荣贺虹*
 (西北农林科技大学 林学院,陕西 杨陵 712100)
Author(s):
 YANG Qing-qing NAN Xiao-ning WANG Yun-guo Guo Xin-rong HE Hong*
 (College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China)
关键词:
 柏大蚜松大蚜细菌16S rRNA-RFLP
Keywords:
Cinara tujafilina C. pinitabulaeformis bacterium 16S rRNA-RFLP
分类号:
S763.306
DOI:
doi:10.3969/j.issn.1001-7461.2015.06.31
文献标志码:
A
摘要:
 利用16S rRNA-RFLP方法分析了同一样地柏大蚜和松大蚜体内细菌的组成。结果表明:2种蚜虫体内细菌主要由其初级内共生菌Buchnera aphidicola和次级内共生菌Candidatus Serratia symbiotica组成,但各自所占的比例存在差异;在柏大蚜克隆文库中,B. aphidicolaCa.Serratia symbiotica分别占37%和56%,还有少量的霍氏肠杆菌(Enterobacter hormaechei)占7%;在松大蚜克隆文库中,B. aphidicola和Ca.Serratia symbiotica分别占66.7%和33.3%。表明2种蚜虫体内的微生物组成比较简单且相似,内共生菌种类相同。
Abstract:
 The bacterial communities within bodies of Cinara tujafilina and C. pinitabulaeformis were analyzed by 16S rRNA-RFLP (Restriction Fragment Length Polymorphism ) method aiming at comparing their endosymbionts composition and differences between C. tujafilina and C. pinitabulaeformis which grow in same site. The results showed that the bacteria in two aphid species were consisted of primary endosymbiont Buchnera aphidicola and secondary endosymbiont Candidatus Serratia symbiotica with different proportions in each clone library. In the clone library of C. tujafilina, B. aphidicola and Ca. Serratia symbiotica accounted for 37% and 56%, respectively, and Enterobacter hormaechei accounted for 7%. In the library of C. pinitabulaeformis, B. aphidicola and Ca. Serratia symbiotica accounted for 66.7% and 33.3%, respectively. The results indicated that the bacterial compositions of the two aphid species were simple and similar, and they had the same kinds of endosymbiosis.

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备注/Memo

备注/Memo:
 收稿日期:2014-12-30 修回日期:2015-03-25
基金项目:国家自然科学基金项目(31070342);林业公益性行业科研专项(201404302-4)。
作者简介:杨青青,女,在读硕士,研究方向:森林昆虫学。E-mail:ldyangqing@163.com
*通信作者:贺虹,女,教授,博士生导师,研究方向:森林昆虫学。E-mail:hehong@nwsuaf.edu.cn
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