[1]何志宇,尹鹏先,程林林,等. 东亚唐棣增殖培养条件的优化[J].西北林学院学报,2016,31(1):126-129.[doi:10.3969/j.issn.1001-7461.2016.01.23]
 HE Zhi-yu,YIN Peng-xian,CHEN Lin-lin,et al. Optimization on Shoot Proliferation Culture Media of Amelanchier asiatica[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(1):126-129.[doi:10.3969/j.issn.1001-7461.2016.01.23]
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 东亚唐棣增殖培养条件的优化()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第1期
页码:
126-129
栏目:
出版日期:
2016-01-31

文章信息/Info

Title:
 Optimization on Shoot Proliferation Culture Media of Amelanchier asiatica
文章编号:
1001-7461(2016)01-0126-04
作者:
 何志宇1尹鹏先1程林林1蔺小丽1蔡靖1姜在民2*
 (1.西北农林科技大学 林学院,陕西 杨陵 712100;2.西北农林科技大学 生命科学学院,陕西 杨陵 712100)
Author(s):
 HE Zhi-yu1YIN Peng-xian1CHEN Lin-lin1LIN Xiao-li1CAI Jing1JIANG Zai-min2*
 (1.College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China;2.College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
 东亚唐棣组织培养优化增殖
Keywords:
Amelanchier asiatica tissue culture optimization proliferation
分类号:
S503.53
DOI:
10.3969/j.issn.1001-7461.2016.01.23
文献标志码:
A
摘要:
 以东亚唐棣(Amelanchier asiatica)无菌试管苗为材料,研究了不同基本培养基、激素组合、蔗糖浓度以及琼脂浓度对东亚唐棣增殖的影响。结果表明:最为适合东亚唐棣增殖的培养基组合为MS+6-BA 1.00 mg·L-1+NAA 0.05 mg·L-1+IBA 0.10 mg·L-1+40 g·L-1蔗糖+8 g·L-1琼脂,增殖系数可达6.83,幼苗叶片生长舒展,苗高和茎的生长量也有了很大提高。试验结果将为东亚唐棣工厂化育苗提供技术指导。
Abstract:
 The objective of this study was to examine the influences of different basic media,hormone combination,sucrose concentration,agar concentration on shoot proliferation of Amelanchier asiatica.The results showed that an optimum medium was MS supplemented with 1.00 mg·L-1 6-BA ,0.05 mg·L-1 NAA,0.10 mg·L-1 IBA ,40 g·L-1 sucrose and 8 g·L-1 agar,by which the multiplication coefficient reached 6.83.The height and quality of the shoots were greatly improved.This system could be used for rapid mass clonal propagation of A.asiatica for commercial production.

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备注/Memo

备注/Memo:
 收稿日期:2015-03-16修回日期:2015-05-08
基金项目:林业公益性行业科研专项(201204308)。
作者简介:何志宇,男,硕士,研究方向:森林植物。E-mail:1025586032@qq.com
*通信作者:姜在民,男,副教授,研究方向:植物学。E-mail:jiangzmz@163.com
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