[1]康太,武宏豆,逯佰艳,等. 柠条锦鸡儿抗坏血酸过氧化物酶基因(CkAPX)和谷胱甘肽还原酶基因(CkGR) cDNA片段的克隆及表达分析[J].西北林学院学报,2016,31(3):91-97.[doi:10.3969/j.issn.1001-7461.2016.03.15]
 KANG Tai,WU Hong-dou,LU Bai-yan,et al. Cloning and Expression Analysis of Ascorbate Peroxidase (CkAPX) and Glutathione Reductase (CkGR) Partial Sequence from Caragana korshinskii[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(3):91-97.[doi:10.3969/j.issn.1001-7461.2016.03.15]
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 柠条锦鸡儿抗坏血酸过氧化物酶基因(CkAPX)和谷胱甘肽还原酶基因(CkGR) cDNA片段的克隆及表达分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第3期
页码:
91-97
栏目:
出版日期:
2016-05-31

文章信息/Info

Title:
 Cloning and Expression Analysis of Ascorbate Peroxidase (CkAPX) and Glutathione Reductase (CkGR) Partial Sequence from Caragana korshinskii
文章编号:
1001-7461(2016)03-0091-07
作者:
 康太武宏豆逯佰艳龙晓刚龚春梅白娟*
 (西北农林科技大学 生命科学学院,陕西 杨陵 712100)
Author(s):
 KANG TaiWU Hong-douLU Bai-yanLONG Xiao-gangGONG Chun-meiBAI Juan*
 (College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
柠条锦鸡儿抗坏血酸过氧化物酶谷胱甘肽还原酶抗旱性
Keywords:
Caragana korshinskii ascorbate peroxidase glutathione reductase drought tolerance
分类号:
S718.460
DOI:
10.3969/j.issn.1001-7461.2016.03.15
文献标志码:
A
摘要:
 抗坏血酸过氧化物酶(APX)和谷胱甘肽还原酶(GR)是植物体内抗坏血酸-谷胱甘肽循环系统的重要组成部分,是植物清除活性氧(ROS)的主要酶。根据前期对不同干旱地区柠条锦鸡儿叶片的转录组测序结果,克隆了2个与清除过氧化氢相关的基因:抗坏血酸过氧化物酶基因(CkAPX)和谷胱甘肽还原酶基因(CkGR)片段,长度分别为509 bp和519 bp。序列分析表明APX蛋白和GR蛋白在不同物种中相对保守,在已克隆APXGR基因的物种中,柠条APX蛋白与豆科植物蒺藜苜蓿(Medicago truncatula)的亲缘关系最近,GR蛋白与豆科植物鹰嘴豆(Cicer arietinum)的亲缘关系最近。对安塞、神木和东胜的柠条叶片中的CkAPX和CkGR的表达量和酶活性分析表明,随着降雨量的减少干旱叶片中柠条CkAPX和CkGR的表达量和活性均呈上升趋势,说明此2基因在增强柠条锦鸡儿的抗旱性方面起重要作用。
Abstract:
 As the important components of ascorbate-glutathione cycle system,ascorbate peroxidase (APX) and glutathione reductase (GR) are major enzymes for scavenging reactive oxygen species (ROS) in plants.After referring the transcriptome sequencing results in the leaves of Caragana korshinskii collected from different arid regions,the partial sequences of CkAPX and CkGR were cloned,with lengths of 509 bp and 519 bp,respectively.Sequence analysis indicated that the proteins of APX and GR were relatively conservative in different plant species.The phylogenetic tree analysis suggested that C.korshinskii APX presented the closest genetic relationship with Medicago truncatula,while GR had the closest genetic relationship with Cicer arietinum.The analyses of the expression levels and activities of CkAPX and CkGR in C.korshinskii leaves collected from Ansai,Shenmu and Dongsheng (three typical arid regions in Northern Shaanxi) suggested the expression levels and activities increased with the decrease of rainfall,indicating that CkAPX and CkGR played key roles in drought tolerance of C.korshinskii.

相似文献/References:

[1]李彦瑾,赵 忠,孙德祥,等.干旱胁迫下柠条锦鸡儿的水分生理特征[J].西北林学院学报,2008,23(03):1.
 LI Yanjin,Z HAO Zhong,SUN Dexi ang,et al.H ydrologi calPhysiological Characteristics of Caragana korshinskii under Water stress[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(3):1.
[2]周正立,于军,陈加利,等.柠条锦鸡儿和小叶锦鸡儿不同种源苗期生长动态研究[J].西北林学院学报,2011,26(06):74.
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[4]陈丽茹,李秧秧*. 柠条锦鸡儿种子生产和活力对模拟降雨量改变的响应[J].西北林学院学报,2018,33(6):20.[doi:10.3969/j.issn.1001-7461.2018.06.04]
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备注/Memo

备注/Memo:
 收稿日期:2015-12-24修回日期:2016-02-04
基金项目:国家自然科学基金(31100455)。
作者简介:康太,男,硕士研究生,研究方向:植物抗逆分子生物学。E-mail:635351091@qq.com
*通信作者:白娟,女,博士,副教授,研究方向:植物抗逆分子生物学。E-mail:baijuan@nwsuaf.edu.cn
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