[1]钱瑭璜,刘建华,雷江丽*.转胡杨PeDREB2b基因白三叶株系耐盐性研究[J].西北林学院学报,2016,31(3):109-113.[doi:10.3969/j.issn.1001-7461.2016.03.18]
QIAN Tang-huang,LIU Jian-hua,LEI Jiang-li*. Salt Resistance of Transgenic White Clover (Trifolium repens) Lines with PeDREB2b Gene from Populus euphratica[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(3):109-113.[doi:10.3969/j.issn.1001-7461.2016.03.18]
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转胡杨PeDREB2b基因白三叶株系耐盐性研究()
《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]
- 卷:
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第31卷
- 期数:
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2016年第3期
- 页码:
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109-113
- 栏目:
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- 出版日期:
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2016-05-31
文章信息/Info
- Title:
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Salt Resistance of Transgenic White Clover (Trifolium repens) Lines with PeDREB2b Gene from Populus euphratica
- 文章编号:
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1001-7461(2016)03-0109-05
- 作者:
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钱瑭璜1; 2; 刘建华2; 雷江丽1*
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(1.深圳市 中科院 仙湖植物园,广东 深圳 518004;2.深圳市铁汉生态环境股份有限公司,广东 深圳 518040)
- Author(s):
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QIAN Tang-huang1; 2; LIU Jian-hua2; LEI Jiang-li1*
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(1.Fairylake Botanical Garden,CAS and Shenzhen,Shenzhen,Guangdong 518004,China; 2.Shenzhen Techand Eeology & Environment Co.,Ltd.Shenzhen,Guangdong 518040,China)
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- 关键词:
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白三叶; 转基因株系; 耐盐性
- Keywords:
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white clover; transgenic line; salt resistance
- 分类号:
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S722.39
- DOI:
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10.3969/j.issn.1001-7461.2016.03.18
- 文献标志码:
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A
- 摘要:
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以W1-3、W28-2、W33-3等3个转胡杨PeDREB2b基因白三叶(Trifolium repens)株系为材料,非转基因白三叶海发为对照,测定了植株在不同浓度和不同时间盐胁迫下的盐害指数、存活率、叶片相对电导率、植株地上及地下部分Na+、K+含量变化、以及Na+/K+,从而评价转基因白三叶株系的耐盐能力。结果表明:1)白三叶“海发”及其转基因的3个株系在不同盐浓度胁迫下30 d存活率均为100%。胁迫过程中受害程度随着胁迫浓度增加和时间的延长而加重,高浓度胁迫30 d后,株系W33-3的盐害指数达67%,其他株系为40%左右。2)各株系叶片相对电导率随胁迫时间延长呈不同程度的增加趋势,盐胁迫30 d后,植株地上及地下部分Na+浓度呈显著升高,K+则呈降低趋势,Na+/K+比升高。3)采用隶属函数法综合分析,白三叶“海发”及其转基因的3个株系耐盐性强弱依次为W1-3>HF>W28-2>W33-3。
- Abstract:
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Three transgenic lines were selected from transgenic white clover (Trifolium repens) obtained before,including W1-3,W28-2 and W33-3,while non-transgenic one was used as the control to examine their salt resistance.Salt injury indexes,relative electric conductivity,content variations of Na+,K+ and the ratios of Na+/K+ were measured under salt stress.The results showed that:1) After 30 days’ salt stress under different levels,the survival rate of transgenic white clover was 100%.With the increase of salt concentration and time,the salt injury index of W33-3 was 67%,and more than 40% for other lines.2) The relative electric conductivity of the stressed plants increased in different degrees with time.After 30 days,the content of Na+ significantly increased,while the content of K+ decreased,resulting in the increase of the ratio of Na+/K+.3) Through comprehensive analysis,it was concluded that the salt resistant capabilities of these three transgenic lines ranked as W1-3>Hf>W28-2>W33-3.
备注/Memo
- 备注/Memo:
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收稿日期:2015-07-10修回日期:2015-10-09
基金项目:深圳市科技计划项目(GCZX20120618100801416);广东省科技计划项目(2014B090903015)。
作者简介:钱瑭璜,女,工程师,研究方向:园林植物应用与植物生理。E-mail:582546461@qq.com
*通信作者:雷江丽,女,教授级高工,研究方向:园林植物资源评价与利用。E-mail:lei-jl@163.com
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