[1]王梦,康永祥*,王富,等. 黄帝陵古侧柏花粉生活力及其保存方法的研究[J].西北林学院学报,2016,31(6):133-139.[doi:10.3969/j.issn.1001-7461.2016.06.23]
　WANG Meng,KANG Yong-xiang*,WANG Fu,et al. Viability and Storage Methods of Ancient Platycladus orientalis Pollen in Huangdi Mausoleum[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(6):133-139.[doi:10.3969/j.issn.1001-7461.2016.06.23]
点击复制

 黄帝陵古侧柏花粉生活力及其保存方法的研究()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第6期
页码:
133-139
栏目:
出版日期:
2016-11-30

文章信息/Info

Title:
 Viability and Storage Methods of Ancient Platycladus orientalis Pollen in Huangdi Mausoleum
文章编号:
1001-7461(2016)06-0133-07
作者:
 王梦; 康永祥*; 王富; 甘明旭
 （西北农林科技大学 林学院，陕西 杨陵 712100）
Author(s):
 WANG Meng; KANG Yong-xiang*; WANG Fu; GAN Ming-xu
 (College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
 ; 黄帝陵; 古侧柏; 花粉; 生活力; 保存方法; 超低温保存
Keywords:
 ; Huangdi Mausoleum;  ancient Platycladus orientalis;  pollen;  viability;  storage method;  cryopreservation
分类号:
S791.38
DOI:
10.3969/j.issn.1001-7461.2016.06.23
文献标志码:
A
摘要:
 以黄帝陵千年古侧柏花粉为试验材料，用离体萌发法测定花粉的生活力，研究不同保存方法对花粉萌发率的影响，探讨花粉含水量、投入方式、化冻方式对超低温保存效果的影响。结果表明：古侧柏花粉在9%蔗糖+0.01%硼酸的培养基中离体培养4 d萌发率最高。90 d内，随着保存时间的延长，室温（20℃）保存的花粉迅速失活，低温（0℃、-20℃）保存的花粉萌发率呈逐渐下降趋势，超低温（-196℃）保存的花粉萌发率最高且保持效果最好，为古侧柏花粉长期保存的适宜方法。在超低温保存中，花粉以未经干燥、直接投入液氮、37℃恒温水浴化冻5 min保存效果最好；花粉含水量、投入方式、化冻方式之间交互效应显著。
Abstract:
 The pollen viability of ancient Platycladus orientalis in Huangdi Mausoleum were tested by in vitro culture method to study the effect of different storage methods on pollen germination and to explore the effect of pollen water content,input and thawing method on the pollen cryopreservation method.The results showed that the pollen germination rate reached the highest after cultured 4 days on the culture medium supplemented with 9% sucrose and 0.01% boric acid.In 90 days,with the extension of storage time,the pollen stored at 20 ℃ lost its viability quickly,0℃ and -20℃ could protect the viability of pollen but showed a downward trend overall,cryopreservation had the highest pollen germination rate and kept the best which was the optimal way to achieve the long-term preservation of the ancient P.orientalis pollen.In cryopreservation,pollen without drying was directly put into the liquid nitrogen,thawing at 37℃ water to achieve the best storage effect.The interaction effect between pollen water content,input and thawing method was significant.

相似文献/References:

[1]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
　LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(6):21.
[2]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
　ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(6):38.
[3]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
　YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[4]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
　JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[5]牛晓霆,王逢瑚,曹新民. 明清家具匠师原木下料的工艺原则及措施[J].西北林学院学报,2013,28(01):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
　NIU Xiao-ting,WANG Feng-hu,CAO Xin-min. Techniques and Principles of Log Cuttings of Furniture Makers in Ming and Qing Dynasties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
[6]吕金阳,覃卓凯,舒辉,等. 银杉木材构造美学价值[J].西北林学院学报,2013,28(01):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
　LV Jin-yang,QIN Zhuo-kai,SHU Hui,et al. Esthetical Values in Wood Structure of Cathaya argyrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
[7]杨艳红,张海敏,孙景荣. 基于传统文化视角的现代家具设计探析[J].西北林学院学报,2013,28(01):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
　YANG Yan-hong,ZHANG Hai-min,SUN Jing-rong. Study of Modern Furniture Design Based on Traditional Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
[8]黄圣游,叶喜. 新中式家具“中式”传统的界定[J].西北林学院学报,2013,28(01):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
　HUANG Sheng-You,YE Xi. Definition of Traditional Element in New Chinese Style Furniture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
[9]韩维生,赵明磊,王宏斌. 基于阈限理论的设计尺度体系[J].西北林学院学报,2013,28(01):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
　HAN Wei-sheng,ZHAO Ming-lei,WANG Hong-bin. Design Scale System Based on Threshold Theory[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
[10]宋杰,侯建军,申黎明. 按摩椅按摩头包覆层对按摩舒适性的影响[J].西北林学院学报,2013,28(01):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
　SONG Jie,HOU Jian-jun,SHEN Li-ming. Influence of Massager Coat Layer of Massage Chairs on the Degree of Comfort[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
[11]杨玲,康永祥*,李小军,等. 黄帝陵古柏群林下天然更新研究[J].西北林学院学报,2015,30(01):82.[doi:doi:10.3969/j.issn.1001-7461.2015.01.13]
　YANG Ling,KANG Yong-xiang*,LI Xiao-jun,et al. Natural Regeneration of the Ancient Platycladus orientalis in the Mausoleum of the Yellow Emperor[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(6):82.[doi:doi:10.3969/j.issn.1001-7461.2015.01.13]
[12]甘明旭,孙涛,康永祥*,等. 应用探地雷达对黄帝陵古柏树干和粗根的研究[J].西北林学院学报,2016,31(4):182.[doi:doi:10.3969/j.issn.1001-7461.2016.04.31]
　GAN Ming-xu,SUN Tao,KANG Yong-xiang*,et al. Examination of the Trunk Cvity and Thick Root Distribution of Acient Platycladus orientalis in the Tomb of Yellow Emperor By Ground Penetrating Radar Technology[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(6):182.[doi:doi:10.3969/j.issn.1001-7461.2016.04.31]
[13]刘星旦,康永祥*,甘明旭,等. 黄帝陵古侧柏树干心腐研究[J].西北林学院学报,2017,32(2):180.[doi:10.3969/j.issn.1001-7461.2017.02.31]
　LIU Xing-dan,KANG Yong-xiang*,GAN Ming-xu,et al. A Study on Trunk Decay of Ancient Platycladus orientalis in Tomb of Yellow Emperor[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(6):180.[doi:10.3969/j.issn.1001-7461.2017.02.31]

备注/Memo

备注/Memo:
 收稿日期：2016-04-05修回日期：2016-06-06
基金项目：国家林业公益性行业科研专项（201404302）。
作者简介：王梦，女，在读硕士，研究方向：古树名木保护。E-mail:meeeng@163.com
*通信作者：康永祥，男，教授，博士，研究方向：树木学及古树名木保护。E-mail:yxkang@nwsuaf.edu.cn
更新日期/Last Update: