[1]张正青,李孟楼*. 花绒寄甲ERR和bHLH基因筛选与序列分析[J].西北林学院学报,2016,31(6):190-196.[doi:10.3969/j.issn.1001-7461.2016.06.33]
 ZHANG Zheng-qing,LI Meng-lou*. Identification and Sequence Analysis of ERR and bHLH Gene in Dastarcus helophoroide Transcriptome[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(6):190-196.[doi:10.3969/j.issn.1001-7461.2016.06.33]
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 花绒寄甲ERRbHLH基因筛选与序列分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第6期
页码:
190-196
栏目:
出版日期:
2016-11-30

文章信息/Info

Title:
 Identification and Sequence Analysis of ERR and bHLH Gene in Dastarcus helophoroide Transcriptome
文章编号:
1001-7461(2016)06-0190-07
作者:
 张正青李孟楼*
 (西北农林科技大学 林学院,陕西 杨陵 712100)
Author(s):
 ZHANG Zheng-qingLI Meng-lou*
 (College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
 花绒寄甲ERRbHLH系统发育二级结构
Keywords:
 Dastarcus helophoroide ERR bHLH phylogeny relationship secondary structure
分类号:
S763.3
DOI:
10.3969/j.issn.1001-7461.2016.06.33
文献标志码:
A
摘要:
 采用Blast X比对、ORFfinder查询开放阅读框、MEGA建立Neighbour-Joining系统发育树、EXPASY分析序列组成及ProtScale和HNN预测氨基酸序列的基本性质及二级结构等生物信息学手段,对花绒寄甲的ERRbHLH基因研究,共得到具有完整开放阅读框的ERR序列1条和bHLH序列5条,其氨基酸序列二级结构均由α-螺旋、延伸链、无规则卷曲组成,并确定花绒寄甲bHLH转录因子基序长度为52~55 aa。花绒寄甲ERRbHLH基因在转录组中得到确定,它们在进化关系中与鞘翅目模式昆虫赤拟谷盗更为接近,花绒寄甲的bHLH转录因子基序长度与家蚕和果蝇一致。
Abstract:
 In order to research on ERR and bHLH gene in Dastarcus helophoroides,we used Blast X for sequence blasting,ORFfinder for searching the open reading frame,MEGA method for constructing Neighbour-joining phylogenetic tree,EXPASY for analyzing sequence composition,ProtScale and HNN analysis for analyzing physicochemical properties and forecasting the amino acid secondary structure,and other bioinformatics method.One complete ERR open reading frame and five complete bHLH open reading frames were obtained in total.The secondary structure of ERR and bHLH amino acid sequence consisted of alpha-helix,extended strand and random coil.The length of D.helophoroides bHLH transcription factor sequence motif was 52-55 aa.The ERR and bHLH genes were determined in D.helophoroide stranscriptome,and they were more similar to coleopteran model insect Tribolium castaneum in evolutionary relationship analysis.Moreover,the length of bHLH transcription factor sequence motif in D.helophoroides was consisted with that in Bombyx mori and Drosophila melanogaster.

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备注/Memo

备注/Memo:
 收稿日期:2016-02-28修回日期:2016-06-22
基金项目:国家自然科学基金(31170608)。
作者简介:张正青,女,在读博士,研究方向:森林保护及有害生物防治。E-mail:zhangzhengqing0802@hotmail.com
*通信作者:李孟楼,男,教授,博士生导师,研究方向:森林害虫防治及杀虫剂毒理。E-mail:limenglou@hotmail.com
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