[1]尹跃,安巍,赵建华,等. 枸杞品种SSR荧光指纹图谱构建及遗传关系分析[J].西北林学院学报,2017,32(1):137-141.[doi:10.3969/j.issn.1001-7461.2017.01.22]
 YIN Yue,AN Wei,ZHAO Jian-hua,et al. Fingerprinting and Genetic Diversity Analysis of Wolfberry Cultivars Using Fluorescence-labeled SSR Markers[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(1):137-141.[doi:10.3969/j.issn.1001-7461.2017.01.22]
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 枸杞品种SSR荧光指纹图谱构建及遗传关系分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第1期
页码:
137-141
栏目:
出版日期:
2017-01-31

文章信息/Info

Title:
 Fingerprinting and Genetic Diversity Analysis of Wolfberry Cultivars Using Fluorescence-labeled SSR Markers
文章编号:
1001-7461(2017)01-0137-05
作者:
 尹跃安巍赵建华李彦龙樊云芳曹有龙*
 (宁夏农林科学院 国家枸杞工程技术研究中心,宁夏 银川 750002)
Author(s):
 YIN YueAN WeiZHAO Jian-huaLI Yan-longFAN Yun-fangCAO You-long*
 (Ningxia Academy of Agriculture and Forestry Sciences,National Wolfberry Engineering Research Center,Yinchuan,Ningxia 750002,China)
关键词:
 枸杞SSRDNA指纹图谱遗传多样性
Keywords:
 wolfberry SSR DNA fingerprinting genetic diversity
分类号:
S567.19
DOI:
10.3969/j.issn.1001-7461.2017.01.22
文献标志码:
A
摘要:
 以12个枸杞栽培品种为材料,利用SSR荧光标记进行DNA指纹图谱的构建和遗传关系分析。11对SSR引物在12品种共检测到34个等位变异,平均每个位点为3.1个,位点平均有效等位变异数(Ne)、观测杂合度(Ho)、Shannon’s信息指数(I)和多态信息含量(PIC)分别为2.025、0.439、0.776和0.391。其中4对引物SF1、SF30、SF63和SF92可区分所有供试品种,为品种鉴别提供依据。NTSYS-pc2.10软件聚类表明,12个枸杞品种遗传相似系数变化范围是0.57~0.91,平均为0.68,表明枸杞品种间存在着丰富的遗传多样性。SSR荧光标记技术可以有效地用于枸杞品种资源指纹图谱构建及遗传关系研究。
Abstract:
 Twelve wolfberry cultivars were used as materials to establish DNA fingerprinting and analyzed genetic diversity with simple sequence repeat (SSR) fluorescent marker.A total of 34 alleles were detected by 11 SSR polymorphic markers,with an average of 3.1 alleles per locus.The values of Ne,Ho,I and PIC were 2.025,0.439,0.776,and 0.391,respectively.SF1,SF30,SF63,and SF92 could distinguish all the cultivars.NTSYS-pc 2.10 software was used to analyze genetic diversity of 12 wolfberry cultivars.The results indicated that genetic similarity coefficients of 12 cultivars ranged from 0.57 to 0.91 with an average of 0.68,which showed that there was abundant genetic diversity in wolfberry cultivars.Fluorescence-labeled SSR markers could effectively conduct fingerprinting and genetic diversity analysis for wolfberry cultivars.

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备注/Memo

备注/Memo:
 收稿日期:2016-04-21修回日期:2016-08-18
基金项目:国家“863”计划项目(2013AA102606-04);宁夏农业特色优势产业新品种选育专项(2013NYYZ0101); 宁夏自然科学基金(NZ15123)。
作者简介:尹跃,男,研究实习员,研究方向:枸杞分子标记辅助育种。E-mail:yueyin0112@aliyun.com
*通信作者:曹有龙,男,研究员,研究方向:枸杞生物技术育种。E-mail:youlongchk@163.com
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