[1]贾红梅,王碧玉,刘迪,等. 农杆菌介导CBL基因对菊花品种‘C008’的转化[J].西北林学院学报,2017,32(1):184-189.[doi:10.3969/j.issn.1001-7461.2017.01.29]
　JIA Hong-mei,WANG Bi-yu,LIU Di,et al. Agrobacterium-Mediated Transformation of Chrysanthemum morifolium ‘C008’ With CBL Gene[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(1):184-189.[doi:10.3969/j.issn.1001-7461.2017.01.29]
点击复制

 农杆菌介导CBL基因对菊花品种‘C008’的转化()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第1期
页码:
184-189
栏目:
出版日期:
2017-01-31

文章信息/Info

Title:
 Agrobacterium-Mediated Transformation of Chrysanthemum morifolium ‘C008’ With CBL Gene
文章编号:
1001-7461(2017)01-0184-06
作者:
 贾红梅; 王碧玉; 刘迪; 毛洪玉*
 （沈阳农业大学 林学院，辽宁 沈阳 110161）
Author(s):
 JIA Hong-mei; WANG Bi-yu; LIU Di; MAO Hong-yu*
 （College of Forestry, Shenyang Agricultural University, Shenyang, Liaoning 110161, China）
关键词:
 ; 菊花; CBL基因; 农杆菌介导; 遗传转化
Keywords:
 ; chrysanthemum;  CBL gene;  agrobacterium-mediated;  genetic transformation
分类号:
S682.11
DOI:
10.3969/j.issn.1001-7461.2017.01.29
文献标志码:
A
摘要:
 以菊花品种‘C008’无菌苗叶片作为外植体，在建立了菊花高效再生体系的基础上，通过农杆菌介导法将CBL基因导入菊花品种‘C008’中。试验共获得抗性植株41株，PCR检测表明，阳性植株为5株，证明CBL基因已转化到菊花‘C008’的基因组DNA中，转化率为12.2%。遗传转化过程中，预培养2 d后，将OD600=0.5~0.6的农杆菌稀释50倍后侵染叶片8 min，再共培养2 d、延迟筛选2 d，有利于获得较高的转化率。
Abstract:
 On the basis of a highly efficient regeneration system of chrysanthemum, the CBL gene was introduced into chrysanthemum ‘C008’ by agrobacterium mediated method based on the chrysanthemum ‘C008’ aseptic seedling leaf as explants. In the experiment, 41 strains of resistant plants were obtained. The PCR test showed that the positive plants were 5, which proved that the CBL gene had been transformed into the genome DNA of the chrysanthemum ‘C008’, and the transformation rate was 12.2%. In the process of genetic transformation, after pre-culture for 2 d, the explants were infected for 8 min with diluted OD600=0.5-0.6 Agrobacterium culture liquild with 50 times, and then co-cultured for 2 d, delayed screening for 2 d, which was beneficial to obtain a higher transformation rate.

相似文献/References:

[1]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
　LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(1):21.
[2]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
　ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(1):38.
[3]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
　YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(1):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[4]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
　JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(1):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[5]牛晓霆,王逢瑚,曹新民. 明清家具匠师原木下料的工艺原则及措施[J].西北林学院学报,2013,28(01):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
　NIU Xiao-ting,WANG Feng-hu,CAO Xin-min. Techniques and Principles of Log Cuttings of Furniture Makers in Ming and Qing Dynasties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(1):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
[6]吕金阳,覃卓凯,舒辉,等. 银杉木材构造美学价值[J].西北林学院学报,2013,28(01):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
　LV Jin-yang,QIN Zhuo-kai,SHU Hui,et al. Esthetical Values in Wood Structure of Cathaya argyrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(1):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
[7]杨艳红,张海敏,孙景荣. 基于传统文化视角的现代家具设计探析[J].西北林学院学报,2013,28(01):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
　YANG Yan-hong,ZHANG Hai-min,SUN Jing-rong. Study of Modern Furniture Design Based on Traditional Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(1):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
[8]黄圣游,叶喜. 新中式家具“中式”传统的界定[J].西北林学院学报,2013,28(01):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
　HUANG Sheng-You,YE Xi. Definition of Traditional Element in New Chinese Style Furniture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(1):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
[9]韩维生,赵明磊,王宏斌. 基于阈限理论的设计尺度体系[J].西北林学院学报,2013,28(01):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
　HAN Wei-sheng,ZHAO Ming-lei,WANG Hong-bin. Design Scale System Based on Threshold Theory[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(1):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
[10]宋杰,侯建军,申黎明. 按摩椅按摩头包覆层对按摩舒适性的影响[J].西北林学院学报,2013,28(01):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
　SONG Jie,HOU Jian-jun,SHEN Li-ming. Influence of Massager Coat Layer of Massage Chairs on the Degree of Comfort[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(1):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]

备注/Memo

备注/Memo:
 收稿日期：2016-05-24修回日期：2016-08-19
基金项目：辽宁省自然科学基金（2014027004）；辽宁省教育厅科学研究计划（2015483）。
作者简介：贾红梅，女，在读硕士，研究方向：园林植物栽培与应用研究。E-mail：575658983@qq.com
*通信作者：毛洪玉,女，副教授，研究方向：园林植物逆境生理与分子生物学研究。E-mail：maohongyu74@163.com
更新日期/Last Update: