[1]李瑾瑾,张敏*,周鹏,等. 乌饭树茎段组织的石蜡切片制作技术研究[J].西北林学院学报,2017,32(2):174-179.[doi:10.3969/j.issn.1001-7461.2017.02.30]
　LI Jin-jin,ZHANG Min*,ZHOU Peng,et al. Preparation of Paraffin Section of Vaccinium bracteatum Stem Segment Tissue[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(2):174-179.[doi:10.3969/j.issn.1001-7461.2017.02.30]
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 乌饭树茎段组织的石蜡切片制作技术研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第2期
页码:
174-179
栏目:
出版日期:
2017-03-31

文章信息/Info

Title:
 Preparation of Paraffin Section of Vaccinium bracteatum Stem Segment Tissue
文章编号:
1001-7461(2017)02-0174-06
作者:
 李瑾瑾1; 张敏2*; 周鹏2; 方炎明1*
 (1.南京林业大学 南方现代林业协同创新中心，江苏 南京 210037；2.江苏省林业科学研究院，江苏 南京 211153)
Author(s):
 LI Jin-jin1; ZHANG Min2*; ZHOU Peng2; FANG Yan-ming1*
 （1.South Modern Forestry Cooperative Innovation Center,Nanjing Forestry University,Nanjing,Jiangsu 210037,China; 2.Jiangsu Academy of Forestry,Nanjing,Jiangsu 211153,China）
关键词:
 ; 乌饭树; 茎段; 石蜡切片; 显微观察
Keywords:
 ; Vaccinium bracteatum;  stem segment;  paraffin section;  microscopic observation
分类号:
S685
DOI:
10.3969/j.issn.1001-7461.2017.02.30
文献标志码:
A
摘要:
 以乌饭树（Vaccinium bracteatum）嫩枝插条为试验材料，对其取材、FAA及PFA固定液的不同固定、乙二胺及甘油乙醇溶液的不同软化处理、脱水、透明、浸蜡、包埋、切片、染色等程序进行了试验探究。结果表明，2种固定液均适宜作为乌饭树插条的固定液，乙二胺软化效果较好，染色15~20 s为宜。通过试验掌握了乌饭树茎段的石蜡切片制作方法，得出了乌饭树茎段石蜡切片制作过程中的关键技术。
Abstract:
 In the present study,the procedures for preparing paraffin section of V.bracteatum shoot cuttings were investigated.Different treatments,including drawing materials,FAA and PFA fixing solution,softening treatment with ethylenediamine and glycerol ethanol solution,dewatering,transparence,waxing,embedding,section,dyeing etc were also explored.The results showed that the two kinds of fixing solution were both suitable for the fixation of V.bracteatum stem segments.Meanwhile,softening with ethylenediamine got better effect,and the suitable dyeing time was 15-20 s.Through these experiments,the procedure of paraffin section of V.bracteatum was summarized,and the key steps in the procedure were achieved.

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备注/Memo

备注/Memo:
 收稿日期：2016-08-03修回日期：2016-11-04
基金项目：江苏省林业三新工程项目（LYSX［2016］38）；江苏省科技支撑计划项目（BE2014417）。
作者简介：李瑾瑾，女，在读硕士，研究方向：树木生理生化。E-mail:1564363727@qq.com
*通信作者：张敏，女，副研究员，研究方向：林木花卉良种繁育。E-mail:nmzhang@163.com
方炎明，男，教授，博士生导师，研究方向：森林植物学。E-mail:jwu4@njfu.edu.cn
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