[1]杨苗苗,葛含静,李璟琦,等. 苹果园中脂环酸芽孢杆菌的分离及鉴定[J].西北林学院学报,2017,32(2):202-204.[doi:10.3969/j.issn.1001-7461.2017.02.34]
　YANG Miao-miao,GE Han-jing,LI Jing-qi,et al. Isolation and Identification of Alicylobacillus acidoterrestris in Apple Orchard[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(2):202-204.[doi:10.3969/j.issn.1001-7461.2017.02.34]
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 苹果园中脂环酸芽孢杆菌的分离及鉴定()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第2期
页码:
202-204
栏目:
出版日期:
2017-03-31

文章信息/Info

Title:
 Isolation and Identification of Alicylobacillus acidoterrestris in Apple Orchard
文章编号:
1001-7461(2017)02-0202-03
作者:
 杨苗苗1; 葛含静1; 李璟琦1; 李白存1; 南小宁2*; 丁金英3; 翟文俊1*
 （1.陕西学前师范学院，陕西 西安 710100；2.西北农林科技大学，陕西 杨陵 712100； 3.宁夏职业技术学院，宁夏 银川 750021）
Author(s):
 YANG Miao-miao1; GE Han-jing1; LI Jing-qi1; LI Bai-cun1; NAN Xiao-ning2*; DING Jin-ying3; ZHAI Wen-jun1*
 (1.Shaanxi Xueqian Normal University,Xian,Shaanxi 710100,China; 2.Northwest A&F University,Yangling,Shaanxi 712100,China; 3.Ningxia Polytechnic,Yinchuan,Ningxia 750021,China)
关键词:
 ; 脂环酸芽孢杆菌; 苹果汁; 苹果园土壤; 腐烂落果
Keywords:
Alicylobacillus acidoterrestris;  apple juice;  apple orchard soil;  dropped apple
分类号:
S763.1
DOI:
10.3969/j.issn.1001-7461.2017.02.34
文献标志码:
A
摘要:
 分别从苹果园土壤和腐烂落果中分离到了9株和5株嗜酸耐热菌，树冠上完整无损的健康苹果中未分离到，分离到的菌均为芽孢杆菌；菌落圆形，直径1.0~3.0 mm，中心稍凸起，有同心圆，半透明，不易挑起；以已知菌Alicyclobacillus acidoterretris为阳性对照，在分离到的14株菌中均扩增到了294 bp的目标条带，分子鉴定结果证明分离菌属于脂肪酸芽孢杆菌；该研究确定苹果汁中的嗜酸耐热菌最初来源于苹果园土壤。
Abstract:
 Nine and five Alicylobacillus acidoterrestris were isolated respectively from the soil and dropped apples in orchard,and A.acidoterrestris was not found in healthy apple.The isolates were Bacillus,colony was transparent,roughly round,1.0-3.0 mm in diameter,protrusion formed in the center,and difficult for collection.The 294 bp fragments were amplified from the isolates,suggesting that the isolates belong to Alicylobacillus spp..The results showed that the A.acidoterrestris found from apple juice was originated from orchard soil.

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备注/Memo

备注/Memo:
 收稿日期：2016-06-27修回日期：2016-10-26
基金项目：陕西省教育厅科研计划项目（15JK1183）；陕西省科技计划项目（2016NY-204,2016JQ3036）；学前师范学院引进人才科研启动基金（2014DS026）；校级基金项目（2015ZDKJ007）。
作者简介：杨苗苗，女，博士，助理研究员，研究方向：植物保护及其生物制品。E-mail:yangmmgg@163.com
*通信作者：南小宁，男，博士，讲师，研究方向：森林保护。E-mail:nxn@nwsuaf.edu.cn
翟文俊，男，研究员，研究方向：食品加工及生物制品。
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