[1]史倩倩,周琳,王雁*,等. 黄牡丹PlWDR3和PlWDR18转录因子基因的克隆与表达[J].西北林学院学报,2017,32(3):97-103.[doi:10.3969/j.issn.1001-7461.2017.03.18]
　SHI Qian-qian,ZHOU Lin,WANG Yan*,et al. Isolation and Expression of PlWDR3 and PlWDR18 Transcription Factor Genes in Paeonia lutea[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(3):97-103.[doi:10.3969/j.issn.1001-7461.2017.03.18]
点击复制

 黄牡丹PlWDR3和PlWDR18转录因子基因的克隆与表达()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第3期
页码:
97-103
栏目:
出版日期:
2017-05-31

文章信息/Info

Title:
 Isolation and Expression of PlWDR3 and PlWDR18 Transcription Factor Genes in Paeonia lutea
文章编号:
1001-7461(2017)03-0097-07
作者:
 史倩倩1; 2; 周琳2; 王雁2*; 翟立娟1; 李龙1
 （1.西北农林科技大学 风景园林艺术学院， 陕西 杨陵 712100；2.中国林业科学研究院 林业研究所,国家林业局 林木培育重点实验室,北京 100091）
Author(s):
 SHI Qian-qian1; ZHOU Lin2; WANG Yan2*; ZHAI Li-juan1; LI Long1
 (1.College of Landscape Architecture and Arts,Northwest A&F University,Yangling,Shaanxi 712100,China; 2.Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Research Institute of Forestry,CAF,Beijing 100091,China)
关键词:
 ; 黄牡丹; WDR40; 花色素合成; 基因克隆; 表达分析
Keywords:
Paeonia lutea;  WDR40;  anthocyanin biosynthesis;  gene cloning;  expression analysis
分类号:
S718.46
DOI:
10.3969/j.issn.1001-7461.2017.03.18
文献标志码:
A
摘要:
 以云南野生黄牡丹为试验材料，根据已构建的云南野生黄牡丹花瓣转录组数据库提供的WDR40蛋白的Unigene筛选得到19个与花色素合成相关的WDR40蛋白同源性高的Unigene序列，命名为PlWDR1~19.通过氨基酸序列比较和系统进化树分析，发现PlWDR3和PlWDR18可能参与调控黄牡丹的花色素合成。PlWDR3的ORF包含1个1 032 bp的开放阅读框，编码1个344个氨基酸的蛋白，与苹果MdTTG1的亲缘关系最近，相似性达83.29%；PlWDR18的ORF包含1个1 035 bp的开放阅读框，编码1个345个氨基酸的蛋白，与葡萄VvWDR2相似性最高，达93.77%。相对荧光定量PCR分析表明，PlWDR3在黄牡丹和紫牡丹的不同时期的表达模式基本相似，在黄牡丹和紫牡丹圆桃期表达量最高，在初开期表达量最低；PlWDR18在黄牡丹花发育初期呈上升趋势，在透色期达到最高峰，然后下降，在初开期达到最小值；在紫牡丹花发育初期亦呈上升趋势，在圆桃期达到最大值，然后逐渐下降，在盛开期降到最小值。PlWDR18在黄牡丹和紫牡丹花的各个发育时期的表达量均高于PlWDR3的表达量。推测PlWDR3和PlWDR18参与黄牡丹花色形成的调控，为今后深入探讨黄牡丹花色形成机制奠定基础。
Abstract:
 In this study,19 unigene sequences that shared high homology with WDR40 transcription factor protein involved in plant anthocyanin biosynthesis were obtained from previous-constructed petal transcriptome database of Paeonia lutea and named PlWDR1-19.PlWDR3 and PlWDR18 were considered to be related to regulate anthocyanin biosynthesis by comparing analysis of amino acid sequence and phylogenetic tree analysis.And PlWDR3 contained a 1 032 bp ORF encoding 344 amino acid residues and PlWDR18 contained a 1 035 bp ORF encoding 345 amino acid residues，which both contained the typical WD40 structural domain.The predicted protein sequence of PlWDR3 shared high similarity with MdTTG1 (83.29%),and PlWDR18 shared 93.77% similarity with VvWDR2.Relative real-time PCR analysis indicated that PlWDR3 showed similar expression pattern in different periods of P.lutea and P.delavayi,and reached the highest abundance at stage 2 while it reached the lowest level at stage 3.And PlWDR18 rose at the beginning stages of P.lutea with the peak at stage 3 and then declined while it had the highest expression level at stage 2 and then declined with the lowest level at stage 5.The results also showed that PlWDR18 expressed the higher abundance than PlWDR3 in all samples.In conclusion,we inferred that PlWDR3 and PlWDR18 might associate with the regulatory of anthocyanin biosynthesis in P.lutea and this would provide the basis for insight into the molecular mechanisms underlying tree peony yellow flower pigmentation.

相似文献/References:

[1]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
　LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(3):21.
[2]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
　ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(3):38.
[3]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
　YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(3):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[4]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
　JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(3):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[5]牛晓霆,王逢瑚,曹新民. 明清家具匠师原木下料的工艺原则及措施[J].西北林学院学报,2013,28(01):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
　NIU Xiao-ting,WANG Feng-hu,CAO Xin-min. Techniques and Principles of Log Cuttings of Furniture Makers in Ming and Qing Dynasties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(3):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
[6]吕金阳,覃卓凯,舒辉,等. 银杉木材构造美学价值[J].西北林学院学报,2013,28(01):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
　LV Jin-yang,QIN Zhuo-kai,SHU Hui,et al. Esthetical Values in Wood Structure of Cathaya argyrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(3):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
[7]杨艳红,张海敏,孙景荣. 基于传统文化视角的现代家具设计探析[J].西北林学院学报,2013,28(01):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
　YANG Yan-hong,ZHANG Hai-min,SUN Jing-rong. Study of Modern Furniture Design Based on Traditional Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(3):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
[8]黄圣游,叶喜. 新中式家具“中式”传统的界定[J].西北林学院学报,2013,28(01):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
　HUANG Sheng-You,YE Xi. Definition of Traditional Element in New Chinese Style Furniture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(3):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
[9]韩维生,赵明磊,王宏斌. 基于阈限理论的设计尺度体系[J].西北林学院学报,2013,28(01):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
　HAN Wei-sheng,ZHAO Ming-lei,WANG Hong-bin. Design Scale System Based on Threshold Theory[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(3):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
[10]宋杰,侯建军,申黎明. 按摩椅按摩头包覆层对按摩舒适性的影响[J].西北林学院学报,2013,28(01):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
　SONG Jie,HOU Jian-jun,SHEN Li-ming. Influence of Massager Coat Layer of Massage Chairs on the Degree of Comfort[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(3):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]

备注/Memo

备注/Memo:
 收稿日期：2016-10-09修回日期：2017-01-17
基金项目：国家“863”计划(2011AA10020701)；西北农林科技大学2015年博士科研启动项目（Z109021611）。
作者简介：史倩倩，女，讲师，博士，研究方向：花卉遗传改良。E-mail:shiqianqian2005@163.com
*通信作者：王雁，女，研究员，博士，博士生导师，研究方向：园林植物应用。E-mail:chwy8915@sina.com
更新日期/Last Update: