[1]贺圆,王兵益,廖声熙*,等. 云南松基因组微卫星序列特征分析[J].西北林学院学报,2017,32(3):104-107.[doi:10.3969/j.issn.1001-7461.2017.03.19]
　HE Yuan,WAND Bing-yi,LIAO Sheng-xi*,et al. Characteristic Analysis of Microsatellites of Pinus yunnanensis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(3):104-107.[doi:10.3969/j.issn.1001-7461.2017.03.19]
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 云南松基因组微卫星序列特征分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第3期
页码:
104-107
栏目:
出版日期:
2017-05-31

文章信息/Info

Title:
 Characteristic Analysis of Microsatellites of Pinus yunnanensis
文章编号:
1001-7461(2017)03-0104-04
作者:
 贺圆1; 2; 王兵益2; 廖声熙2*; 崔凯2
 （1.西南林业大学，云南 昆明 650224；2.中国林业科学研究院 资源昆虫研究所，云南 昆明 650224）
Author(s):
 HE Yuan1; 2; WAND Bing-yi2; LIAO Sheng-xi2*; CUI Kai2
 (1.Southwest Forestry University， Kunming,Yunnan 650224,China; 2.Research Institute of Resources Insects， CAF， Kunming,Yunnan 650224,China)
关键词:
 ; 云南松; 基因组; 微卫星; 重复类型
Keywords:
Pinus yunnanensis;  genome;  microsatellite;  repeat type
分类号:
S791.257
DOI:
10.3969/j.issn.1001-7461.2017.03.19
文献标志码:
A
摘要:
 为了更好地了解云南松基因组特征，分析了云南松基因组中微卫星序列的分布特征，采用FLAF-seq测序技术，共获得1 970条微卫星重复序列，利用MISA通过对Unigene序列的分析，鉴定出7种类型的SSR。7种微卫星序列中主要以单碱基重复类型为主，共1 104个，约占重复序列总数的56.04%，其次是双碱基和三碱基重复类型，分别占总数的27.66%和11.68%，四碱基重复类型数量30个，占重复序列总数的1.52%，五碱基、六碱基重复类型在总重复序列中占比最少，均为0.2%，混合微卫星占2.7%。在单碱基重复类型中占优势的重复单元是A/T碱基，双碱基重复类型中占优势的重复单元是(AT)n/(TA)n碱基，三、四、五碱基重复类型中，占优势的重复单元不明显，但几种碱基重复类型中，都包含着丰富的A和T。所得到的微卫星重复序列中以长度＜20 bp序列的微卫星数量最多，微卫星长度≥20 bp的只占总数的10.81%。可为后续云南松遗传多样性的研究及SSR引物筛选等提供一定的理论依据。
Abstract:
 In order to better understand the genomic features of Pinus yunnanensis,FLAF-seq sequencing technology was used to analyze the distribution and variation of SSR from genomic DNA of P.yunnanensis.Totally 1 970 microsatellite repeats were identified by using MIcroSAtellite identification tool.Through the analysis of unigene sequence,seven types of SSR were recognized.Among them,mono-nucleotide repeat types had the largest number (1 104),accounting for 56.04% of the total repeating sequences,followed by di-nucleotide and tri-nucleotide repeat types,accounting for 27.66% and 11.68%,respectively.The number of tetra-nucleotide repeat types was 30 (1.52%),and penta-nucleotide and hexa-nucleotide repeat types were only accounting for 0.2%.Compound SSR accounted for 2.7%.Dominant type in mono-nucleotide repeat types units was A/T,and di-nucleotide repeat types were (AT)n/(TA)n.The dominant repeat units in tri-nucleotide,tetra-nucleotide,penta-nucleotide,and hexa-nucleotide were not obvious.However,all of them contained a wealth of A and T.Among the obtained microsatellite repeat sequences,most of them were less than 20 bp.The microsatellite of more than or equal to 20 bp only occupied 10.81% of the total sequence.This study would provide a theoretical basis for further research of P.yunnanensis genetic diversity and SSR primers screening.

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备注/Memo

备注/Memo:
 收稿日期：2016-05-16修回日期：2016-12-29
基金项目：国家“十二五”科技支撑计划课题(2012BAD01B0203)。
作者简介：贺圆，女，在读硕士，研究方向：森林植物资源与利用。E-mail:jxl836@163.com
*通信作者：廖声熙，男，副研究员，研究方向：森林培育。E-mail:cafliao@163.com
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