[1]汪王,苏小霞,杨柳慧,等. 细叶百合LpSVP基因的克隆及其表达分析[J].西北林学院学报,2018,33(4):89-94.[doi:10.3969/j.issn.1001-7461.2018.04.14]
　WANG Wang,SU Xiao-xia,YANG Liu-hui,et al. Cloning and Expression Analysis of LpSVP Gene from Lilium pumilum[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2018,33(4):89-94.[doi:10.3969/j.issn.1001-7461.2018.04.14]
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 细叶百合LpSVP基因的克隆及其表达分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第33卷
期数:
2018年第4期
页码:
89-94
栏目:
出版日期:
2018-07-31

文章信息/Info

Title:
 Cloning and Expression Analysis of LpSVP Gene from Lilium pumilum
文章编号:
1001-7461(2018)04-0089-06
作者:
 汪王; 苏小霞; 杨柳慧; 周蕴薇*
 （东北林业大学 园林学院，黑龙江 哈尔滨 150000）
Author(s):
 WANG Wang; SU Xiao-xia; YANG Liu-hui; ZHOU Yun-wei*
 （College of Landscape Architecture,Northeast Forestry University,Harbin,Heilongjiang 150000,China)
关键词:
 ; 细叶百合; LpSVP; 花发育
Keywords:
Lilium pumilum;  LpSVP;  flower development
分类号:
S682.29
DOI:
10.3969/j.issn.1001-7461.2018.04.14
文献标志码:
A
摘要:
 从细叶百合（Lilium pumilum）中克隆得到LpSVP基因，该基因ORF全长为675 bp，编码224个氨基酸。序列同源性和系统进化树分析表明该基因属于MADS-box基因家族下SVP类基因；生物信息学分析表明蛋白相对分子量为25.783 kDa，理论等电点为5.62，为亲水蛋白，没有跨膜结构；亚细胞定位预测于细胞核中；蛋白质二级结构a-螺旋占60.71%，随机卷曲占29.02%，延伸链占10.27%；蛋白质三级结构以同型四聚体形式存在。半定量分析表明该基因在鳞茎、叶片、雌蕊、雄蕊和花瓣中均表达，但表达有强弱差异；实时荧光定量分析表明在花发育过程中该基因表达量先增长后下降。该研究为深入了解LpSVP基因功能奠定了基础。
Abstract:
 The LpSVP gene was cloned from Lilium pumilum,the full-length of ORF was 675 bp,and encoding 224 amino acids.Sequence homology and phylogenetic analysis showed that this gene belonged to SVP-like MADS-box genes; bioinformatics analysis showed that the relative molecular weigh of the LpSVP gene was 25.783 kDa,the theoretical isoelectric point was 5.62,and it was hydrophilic protein with no transmembrane structure; the subcellular localization was predicted in nucleus; the secondary structure analysis showed that LpSVP comprised 60.71% of alpha-helix,29.02% random coil and 10.27% extended strains; the tertiary structure of the LpSVP protein existed as isomeric tetramer.RT-PCR analysis showed that the gene was expressed in bulb,leaf,pistil,stamen and petal,but varied widely in the level of expression.Real-time PCR analysis showed that the expression of LpSVP increased firstly and then decreased continuously during flower development.The study would lay a foundation for further understanding the function of the gene.

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备注/Memo

备注/Memo:
 收稿日期：2017-08-26修回日期：2017-10-17
基金项目：国家自然科学基金“低温解除细叶百合鳞茎休眠过程中的糖代谢动态及ADP核糖基化因子的生理功能”（31470698）
作者简介：汪王，女，在读硕士，研究方向：园林植物种质资源。E-mail:1192763410@qq.com
*通信作者：周蕴薇，女，博士后，教授，研究方向：园林植物种质资源。E-mail：boombiubiubiu@126.com
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