[1]张琳,黄志远,苏少文,等. 外源激素对荷花生长及相关基因表达的影响[J].西北林学院学报,2019,34(2):35-41.[doi:10.3969/j.issn.1001-7461.2019.02.06]
　ZHANG Lin,HUANG Zhi-yuan,SU Shao-wen,et al. Effects of Exogenous Hormones on the Growth of Lotus and Expression of Related Genes[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2019,34(2):35-41.[doi:10.3969/j.issn.1001-7461.2019.02.06]
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 外源激素对荷花生长及相关基因表达的影响()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第34卷
期数:
2019年第2期
页码:
35-41
栏目:
出版日期:
2019-03-31

文章信息/Info

Title:
 Effects of Exogenous Hormones on the Growth of Lotus and Expression of Related Genes
文章编号:
1001-7461(2019)02-0035-07
作者:
 张琳; 黄志远; 苏少文; 孟亚南; 刘艺平; 孔德政*
 （河南农业大学 林学院，河南 郑州 450002）
Author(s):
 ZHANG Lin; HUANG Zhi-yuan; SU Shao-wen; MENG Ya-nan; LIU Yi-ping; KONG De-zheng*
（College of Forestry,Henan Agricultural University,Zhengzhou 450002,Henan,China）
关键词:
 ; 荷花; 外源激素; 相关基因; 荧光定量PCR
Keywords:
lotus;  exogenous hormones;  related gene;  real-time quantitative PCR
分类号:
S682.32
DOI:
10.3969/j.issn.1001-7461.2019.02.06
文献标志码:
A
摘要:
 为了探讨植物激素对荷花生长发育的影响，以荷花‘青玉’为试验材料，分析了在赤霉素GA3(90、120、150 mg/L)和细胞分裂素6-BA(100、150、200 mg/L)处理后，荷花生长发育状态、酶活性、丙二醛含量和相关基因表达量的情况。结果表明：1）150 mg/L GA3和100 mg/L 6-BA处理使荷花叶片的叶面积的增加效果最为显著，150 mg/L GA3和150 mg/L 6-BA处理使荷花株高的增长量最大。2）GA3和6-BA处理均有利于提高荷花花瓣的酶活性和丙二醛含量，浓度120 mg/L GA3和150 mg/L 6-BA处理下作用最佳。3）EXP基因的表达水平呈现先上升后下降的趋势，在盛花期达到峰值。而CKX基因表达水平逐渐增加，由盛花期至谢花期表达量迅速提升，达到最大值。表明120 mg/L GA3和150 mg/L 6-BA能有效促进荷花的生长发育，起到显著延长花期的作用。
Abstract:
In order to understand the effects of exogenous hormones on the growth and development of lotus,a lotus cultivar ‘Qingyu’ was treated by gibberellin (GA3) with the concentrations of 90,120,and 150 mg/L,and cytokinin (6-BA) with concentrations of 100,150,and 200 mg/L.Relative parameters were measured,such as the state of plant development,enzyme activity,malondialdehyde content and related gene expression levels.The results showed that：1) the treatments of 150 mg/L GA3 and 100 mg/L 6-BA had the most significant effect on the increase of leaf area.The treatments of 150 mg/L GA3 and 150 mg/L 6-BA resulted in the largest increase of height.2) Both GA3 and 6-BA treatments were beneficial to the increase of the enzyme activity and malondialdehyde content of lotus petal,and the effects were best under the treatments of 120 mg/L GA3 and 150 mg/L 6-BA.3) The expression level of EXP gene increased first,reached the peak value at the full-flowering stage and then decreased afterwards.The expression level of CKX gene gradually increased and rose rapidly from full-flowering stage to flower-wilting stage,reaching the maximum value in full-flowering stage.These results suggested that 120 mg/L GA3 and 150 mg/L 6-BA treatments could effectively promote the growth and development of lotus,and played significant roles in prolonging the flowering period.

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备注/Memo

备注/Memo:
 收稿日期：2018-05-31修回日期：2018-10-16
基金项目：河南省重大科技项目(30601044)。
作者简介：张琳，女，在读硕士，研究方向：园林植物与观赏园艺。E-mail:624209306@qq.com
*通信作者：孔德政，男，博士，教授，研究方向：园林植物遗传育种。E-mail:kzxy303@163.com
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