[1]徐玉洁,王俊儒*. 紫珠草多糖类物质的初步表征及抗补体活性研究[J].西北林学院学报,2019,34(2):194-198.[doi:10.3969/j.issn.1001-7461.2019.02.29]
　XU Yu-jie,WANG Jun-ru*. Preliminary Characterization and Anti-complementary Activity of Polysaccharides from Callicarpa macrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2019,34(2):194-198.[doi:10.3969/j.issn.1001-7461.2019.02.29]
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 紫珠草多糖类物质的初步表征及抗补体活性研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第34卷
期数:
2019年第2期
页码:
194-198
栏目:
出版日期:
2019-03-31

文章信息/Info

Title:
 Preliminary Characterization and Anti-complementary Activity of Polysaccharides from Callicarpa macrophylla
文章编号:
1001-7461(2019)02-0194-05
作者:
 徐玉洁; 王俊儒*
 （西北农林科技大学 化学与药学院，陕西 杨陵 712100）
Author(s):
 XU Yu-jie; WANG Jun-ru*
 (College of Chemistry and Pharmacy,Northwest A&F University,Yangling 712100,Shaanxi,China)
关键词:
 ; 紫珠草; 多糖; 抗补体活性
Keywords:
Callicarpa macrophylla;  polysaccharide;  anti-complementary activity
分类号:
S567.19
DOI:
10.3969/j.issn.1001-7461.2019.02.29
文献标志码:
A
摘要:
 采用水提醇沉法制备紫珠草粗多糖，后经活性炭脱色、Sevage法脱除蛋白质、DEAE-52（OH-）离子交换纤维素分离纯化得到一个紫珠草多糖类物质(ZZC-PC)。对该多糖类物质进行化学测定和光谱分析，应用细胞溶血法进行抗补体活性的研究。结果表明，该物质主要含多糖与木质素，分子量为65.7 ku，具有明显的多糖特征峰；该物质具有较好的抗补体活性，其对经典途径和旁路途径的CH50为(1.95±0.1) mg·mL-1、AP50为(3.3±0.2) mg·mL-1。
Abstract:
 Crude polysaccharide was obtained from Callicarpa macrophylla by water extraction and ethanol precipitation method.Then the crude polysaccharides were purified by active carbon decoloration,sevage deproteinization and DEAE-52 (OH-) ion exchange cellulose to prepare the polysaccharide (ZZC-PC).The polysaccharide was characterized by chemical and spectral analysis and its anti-complementary activity was evaluated by the hemolysis assay.The results showed that ZZC-PC was mainly composed of polysaccharides and lignin possessing obvious absorption peaks of polysaccharide,and the molecular weight (MW) was 65.7 ku.It had potent anti-complementary activity,which inhibited complement activation on both the classic and alternative pathways with CH50 value of (1.95±0.1) mg·mL-1 and AP50 value of (3.3±0.2) mg·mL-1,respectively.

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备注/Memo

备注/Memo:
 收稿日期：2017-12-05修回日期：2018-10-15
基金项目：有机枸杞深加工技术研发和服务（K4030217109）。
作者简介：徐玉洁，女，在读硕士，研究方向：药用植物化学成分。E-mail：xuyj8991@163.com
*通信作者：王俊儒，男，博士，教授，研究方向：植物资源化学和化学生物学。E-mail：wangjr07@163.com
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