[1]李吉祥,陈晓慧,马铭浩,等. 水曲柳FmPAL基因的克隆及表达模式的分析[J].西北林学院学报,2019,34(5):10-16.[doi:10.3969/j.issn.1001-7461.2019.05.02]
　LI Ji-xiang,CHEN Xiao-hui,MA Ming-hao,et al. Cloning and Expression Pattern Analysis of PAL Gene in Fraxinus mandschurica[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2019,34(5):10-16.[doi:10.3969/j.issn.1001-7461.2019.05.02]
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 水曲柳FmPAL基因的克隆及表达模式的分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第34卷
期数:
2019年第5期
页码:
10-16
栏目:
出版日期:
2019-09-30

文章信息/Info

Title:
 Cloning and Expression Pattern Analysis of PAL Gene in Fraxinus mandschurica
文章编号:
1001-7461(2019)05-0010-07
作者:
 李吉祥; 陈晓慧; 马铭浩; 王恒涛; 詹亚光; 曾凡锁*
 （东北林业大学 生命科学学院，黑龙江 哈尔滨 150040）
Author(s):
 LI Ji-xiang; CHEN Xiao-hui; MA Ming-hao; WANG Heng-tao; ZHAN Ya-guang; ZENG Fan-suo*
 (Institute of Life Science,Northeast Forestry University,Harbin 150040,Heilongjiang,China)
关键词:
 ; 水曲柳; FmPAL; 生物信息学分析; 非生物胁迫
Keywords:
Fraxinus mandshurica;  FmPAL;  bioinformatics analysis;  abiotic stress
分类号:
S792.12
DOI:
10.3969/j.issn.1001-7461.2019.05.02
文献标志码:
A
摘要:
 在催化苯丙烷类代谢途径的第一步反应中，苯丙氨酸解氨酶（PAL）起到关键酶和限速酶的作用，对植物有非常重要的生理意义。对水曲柳FmPAL基因进行了生物信息学以及表达模式分析，包括时空及其在NaCl、低温、IAA、ABA以及GA3处理下的特异性表达特征。结果表明，水曲柳FmPAL编码区全长为1 674 bp，编码557个氨基酸。FmPAL蛋白为稳定的亲水性蛋白，不存在信号肽但具有跨膜结构域。FmPAL基因与樟子松的PAL遗传距离比较近，说明它们亲缘关系比较近。FmPAL基因在新生枝和叶中相对表达量高于花、芽、叶柄等部位，并在新生枝中表达量最高，具有组织特异性。水曲柳新生枝和叶片中FmPAL基因在5月表达量相对较高。在非生物胁迫和激素诱导下，FmPAL基因的表达量随处理时间而改变，但其变化趋势不尽相同。在低温（4℃）、盐（NaCl）2种非生物胁迫下，FmPAL基因在处理12 h后表达量最高，分别为对照组的34.3、24.3倍；在低温（4℃）和盐（NaCl）处理处理1 h后，FmPAL基因表达量最低。FmPAL基因在脱落酸（ABA）处理1 h后表达量最高，为对照组的11.9倍;生长素（IAA）处理12 h后表达量最高，为对照组的37.2倍；赤霉素（GA）处理24 h后表达量最高，为对照组的21.5倍；而FmPAL基因在脱落酸（ABA）、生长素（IAA）和赤霉素（GA）处理3 h后表达量皆为最低。研究结果表明FmPAL基因响应非生物胁迫以及植物激素信号诱导。
Abstract:
 In the first step of catalyzing the metabolic pathway of phenylpropanoids,phenylalanine ammonia lyase (PAL) plays a key role in enzymes and rate-limiting enzymes,and has important physiological significance for plants.This study analyzed the bioinformatics and expression pattern of FmPAL gene of Fraxinus mandshurica,including time and space and in low temperature,NaCl,IAA,ABA and GA3 specific expression characteristics under treatment.The results showed that the coding region of FmPAL was 1 674 bp in length and encoded 557 amino acids.FmPAL protein is a stable hydrophilic protein.It has no signal peptide but has transmembrane domain.The genetic distances of FmPAL gene and Olea europaea var.sylvestris were relatively close,indicating that their genetic relationship was relatively close.The relative expressions of FmPAL gene in fresh branches and leaves were higher than those in flowers,buds and petiole,and it was the highest in the new fresh branches.The FmPAL gene in the fresh branches and leaves was relatively high in May.Under the abiotic stress and hormone induction,the expression level of FmPAL gene changed with the treatment time,but the change trend was not the same.Under the abiotic stresses of low temperature (4℃) and salt (NaCl),the expression level of FmPAL gene was the highest after treatment for 12 hours,which was 34.3 times and 24.3 times of that of the control group,respectively.The expression of FmPAL gene was the highest after treatment with abscisic acid (ABA) for 1 hour,which was 11.9 times of that of the control group.The expression of FmPAL gene was the highest after auxin (IAA) treatment for 12 hours,which was 37.2 times of the control group; the expression level was the highest after gibberellin (GA) treatment 24 hours,which was 21.5 times of the control group; while the FmPAL gene was the lowest after 3 hours of treatment with abscisic acid (ABA),auxin (IAA) and gibberellic acid (GA).The results of this study indicated that the FmPAL gene was involved in abiotic stress and phytohormone signal induction.

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备注/Memo

备注/Memo:
 收稿日期：2018-12-03修回日期：2019-04-01
基金项目：中央高校基本科研业务费（2572017DG03）；黑龙江省应用技术研究与开发计划项目(GA19B201)。
作者简介：李吉祥，男，硕士在读，研究方向：植物逆境分子生物学。E-mail：1207289030@qq.com
*通信作者：曾凡锁，男，教授，研究方向：林木遗传改良和逆境分子生物学。E-mail：zengfansuo@126.com
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