[1]王艺,张尚昆,赵翔,等. 核桃TT1类转录因子的筛选及干旱响应分析[J].西北林学院学报,2020,35(1):86-93.[doi:10.3969/j.issn.1001-7461.2020.01.13]
 WANG Yi,ZHANG Shang-kun,ZHAO Xiang,et al. Identification and Expression of the TT1-like Transcription Factor from Juglans regia under Drought Stress[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2020,35(1):86-93.[doi:10.3969/j.issn.1001-7461.2020.01.13]
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 核桃TT1类转录因子的筛选及干旱响应分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第35卷
期数:
2020年第1期
页码:
86-93
栏目:
出版日期:
2020-01-31

文章信息/Info

Title:
 Identification and Expression of the TT1-like Transcription Factor from Juglans regia under Drought Stress
文章编号:
1001-7461(2020)01-0086-08
作者:
 王艺1张尚昆1赵翔1刘玉梅1赵焕元1杨桂燕12马凯恒12*
 (1.西北农林科技大学 林学院 山阳核桃板栗试验示范站,陕西 杨陵 712100; 2.西北农林科技大学 林学院 陕西省经济植物资源开发利用重点实验室,陕西 杨陵 712100)
Author(s):
 WANG Yi1ZHANG Shang-kun1ZHAO Xiang1LIU Yu-mei1ZHAO Huan-yuan1YANG Gui-yan12MA Kai-heng12*
 (1.Walnut and Chestnut Experimental Station of Shanyang,College of Forestry,Northwest A&F University,Yangling 712100,Shaanxi,China; 2.Key Laboratory of Economic Plant Resources Development and Utilization in Shaanxi Province,College of Forestry,Northwest A&F University,Yangling 712100,Shaanxi,China)
关键词:
 核桃TT1基因基因表达干旱胁迫
Keywords:
Juglans regia TT1 gene gene expression drought stress
分类号:
S664.1
DOI:
10.3969/j.issn.1001-7461.2020.01.13
文献标志码:
A
摘要:
 TT1基因(transparent testa1)在植物逆境响应中有重要作用。核桃是重要的经济树种,其产量和质量均受环境影响。为进一步探索核桃(Juglans regia)抗逆机制,以‘香玲’核桃为试验材料,克隆获得核桃JrTT1基因,并进行基因表达和生物信息学分析,预测该基因的基本生物功能。结果表明,JrTT1-1、JrTT1-2、JrTT1-3基因的开放阅读框(OFR)分别为1 014、1 023、1 029 bp,蛋白分子量分别为37 763.51、38 492.94、38 136.36 u,含有氨基酸数分别为337、340、342,理论等电点分别为7.88、7.19、8.89。与其他物种的同源TT1蛋白具有较近的进化关系。且其上游1 200 bp启动子中含有多种与干旱逆境响应相关的顺式作用元件。实时荧光定量PCR(qRT-PCR)发现,JrTT1-1、JrTT1-2、JrTT1-3基因在PEG6000胁迫下能不同程度地被诱导表达,且在叶和根中的表达趋势不同。表明JrTT1基因可以响应干旱胁迫诱导,并具有组织表达特异性,推测其在核桃逆境响应中具有一定作用。
Abstract:
 The TT1 gene (transparent testa 1) plays an important role in plant stress response.Juglans regia is an important economic tree species,and its yield and quality are seriously affected by the environment.In order to further explore the stress resistance mechanism of J.regia,a walnut cultivar ‘Xiangling’ was used as experimental material and JrTT1 gene was identified from walnut transcriptome,then the gene expression and bioinformatics analysis were carried out to predict the basic biological function.The results showed that the open reading frames (OFR) of JrTT1-1,JrTT1-2 and JrTT1-3 genes were 1 014,1 023 and 1 029 bp; the molecular weights of the proteins were 37 763.51,38 492.94 and 38 136.36 u; the amino acids were 337,340 and 342; the theoretical isoelectric point were 7.88,7.19,8.89,respectively.JrTT1-1,JrTT1-2 and JrTT1-3 shared close evolutionary relationship with the homologous TT1 protein of other species.The upstream 1 200 bp promoter of JrTT1-1,JrTT1-2 and JrTT1-3 contained a variety of cis-acting elements associated with drought stress response.Real-time quantitative PCR (qRT-PCR) showed that JrTT1-1,JrTT1-2 and JrTT1-3 could be induced to different degrees under PEG6000 stress,and the expression trends in the leaves and roots were different.It indicated that JrTT1 gene could be induced in response to drought stress and had tissue expression specificity,which was predicted to play a role in walnut stress response.

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备注/Memo

备注/Memo:
 收稿日期:2019-03-02修回日期:2019-05-28
基金项目:国家自然科学基金(31800510);中国博士后科学基金特别资助项目(2017T100782);大学生创新创业训练计划项目(201803072)。
作者简介:王艺,女,本科,研究方向:野生植物资源利用、林产化工。E-mail:Nonowangyi@163.com
*通信作者:马凯恒,女,硕士,实验师,研究方向:林木遗传育种。E-mail:makaiheng@163.com
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