[1]张家君,许珊珊,曹光球,等. 不同氮形态对杉木叶绿素荧光参数和叶绿体超微结构的影响[J].西北林学院学报,2020,35(2):24-31.[doi:10.3969/j.issn.1001-7461.2020.02.04]
　ZHANG Jia-jun,XU Shan-shan,CAO Guang-qiu,et al. Effects of Nitrogen Forms on the Chlorophyll Fluorescence Parameters and Chloroplast Ultra-structure of Cunninghamia lanceolata[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2020,35(2):24-31.[doi:10.3969/j.issn.1001-7461.2020.02.04]
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 不同氮形态对杉木叶绿素荧光参数和叶绿体超微结构的影响()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第35卷
期数:
2020年第2期
页码:
24-31
栏目:
出版日期:
2020-03-31

文章信息/Info

Title:
 Effects of Nitrogen Forms on the Chlorophyll Fluorescence Parameters and Chloroplast Ultra-structure of Cunninghamia lanceolata
文章编号:
1001-7461(2020)02-0024-08
作者:
 张家君1; 2; 许珊珊1; 2; 曹光球1; 2; 林思祖1; 2; 潘彦名1; 叶义全1; 2*
 （1.福建农林大学 林学院，福建 福州 350002；2.国家林业和草业局 杉木工程技术研究中心，福建 福州 350002）
Author(s):
 ZHANG Jia-jun1; 2; XU Shan-shan1; 2; CAO Guang-qiu1; 2; LIN Si-zu1; 2; PAN Yan-ming1; YE Yi-quan1; 2*
 (1.College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China；2.Engineering Research Center of Chinese Fir State Forestry and Grassland Administration,Fuzhou 350002,Fujian,China)
关键词:
 ; 杉木; 超微结构; 叶绿素荧光参数; 偏好性; 氮形态
Keywords:
 ; Cunninghamia lanceolata;  ultrastructure;  chlorophyll fluorescence parameter;  preference;  nitrogen form
分类号:
S718.3
DOI:
10.3969/j.issn.1001-7461.2020.02.04
文献标志码:
A
摘要:
 以1个月生杉木实生苗为试验材料，分析了硝态N、铵态N和铵硝混合（NH4+∶NO3-=3∶1）态N对杉木生长、叶绿素荧光参数和叶绿体超微结构的影响。结果表明：1）不同处理下植株根系和地上部生物量大小表现为铵硝混合处理＞全硝处理＞全铵处理，但不同处理间杉木植株生物量不存在显著差异（P＞0.05）。2）铵硝混合处理下可变荧光强度（Fv）和最大荧光强度（Fm）显著高于全硝和全铵处理，而非化学荧光猝灭系数（nPq）显著低于全铵处理。3）铵硝混合处理下PSⅡ最大光化学效率（Fv/Fm）、PSⅡ潜在活性（Fo/Fv）、光化学猝灭系数（qp）和PSⅡ实际光化学效率值（QY）均显著高于全硝处理，但与铵硝混合处理间不存在显著差异（P＞0.05）。4）叶绿体超微结构结果表明，全硝处理下部分叶绿体膨大隆起，基粒片层排列松散，且数量明显少于全铵和铵硝混合处理，全铵处理显著增加了叶绿体中淀粉粒的数量，而铵硝混合处理中淀粉粒则完全消失，但显著增加了嗜锇颗粒的数量。综上所述，铵硝混合处理一方面能通过增强PSⅡ反应中心电子传递效率和光能的利用能力，促进光合同化产物的积累；另一方面通过抑制淀粉粒的形成，减轻淀粉粒对光合作用的抑制，同时促进光合同化产物的输出，促进杉木幼苗的生长。
Abstract:
 The effects of different forms of nitrogen and its composition,namely NO3--N,NH4+-N and NH4+∶NO3-=3∶1,on the seedlings growth,chlorophyll fluorescence parameters and chloroplast ultra-structure in the leaves of Cunninghamia lanceolata were investigated in this study by using 1-month-old C.lanceolata seedlings.The results showed that:1) the root and shoot biomass of the seedlings under various treatments decreased with the order of NH4+∶NO3-=3∶1＞NO3--N＞NH4+-N,however,the roots and shoots biomass among these treatments did not varied significantly.2) The variable fluorescence (Fv) and maximal fluorescence (Fm) in NH4+∶NO3-=3∶1 treatment were significantly higher than those in NO3--N and NH4+-N treatments,while it was opposite for non-chemical fluorescence quenching (nPq).(3) Moreover,the PSⅡ maximal photochemical efficiency (Fv/Fm),PSⅡ potential efficiency (Fo/Fv),photochemical quenching coefficient (qp) and PSⅡ actual photochemical efficiency (QY) in NH4+∶NO3-=3∶1 treatment were significantly higher than those in NO3--N treatment,while no differences were observed between NH4+∶NO3-=3∶1 and NH4+-N treatments.4) Chloroplast ultra-structure results showed that some of the chloroplasts in NO3--N treatment were swelling bulge and the numbers of grana lamellas were lower than those in NH4+∶NO3-=3∶1 and NO3--N treatments,NH4+- treatment significantly increased the starch granules numbers,while starch granules were not observed in NH4+∶NO3-=3∶1 treatment,and NH4+∶NO3-=3∶1 treatment increased osmiophilic granules numbers in chloroplast.In conclusion,NH4+∶NO3-=3∶1 treatment promotes the accumulation of photoassimilate by enhancing the electron transport efficiency and light energy utilization capacity in PSⅡ reaction center.On the other hand,the treatment alleviates the inhibition of starch granules to photosynthesis by inhibiting their formation,meanwhile,the output of photoassimilates are promoted to accelerate the growth of the seedlings.

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备注/Memo

备注/Memo:
 收稿日期：2019-04-29修回日期：2019-10-19
基金项目：福建省自然科学基金(2017J01608)；福建省高校青年自然基金重点项目(JZ160433)；福建农林大学林学高峰学科建设基金(71201800737)。
作者简介：张家君，女，硕士在读，研究方向：自然资源管理。E-mail：2867890485@qq.com
*通信作者：叶义全，男，博士，讲师，研究方向:林木逆境生理。 E-mail：yeyiquan008@163.com
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