[1]刘一钒,王丹丹*,李晓红. 薄荷Eβf合成酶基因(Tspa11)的克隆、序列分析与表达特性[J].西北林学院学报,2020,35(3):90-94.[doi:10.3969/j.issn.1001-7461.2020.03.14]
 LIU Yi-fan,WANG Dan-dan*,LI Xiao-hong. Effect of Storage at Normal Temperature on the Quality of Zanthoxylum bungeanum[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2020,35(3):90-94.[doi:10.3969/j.issn.1001-7461.2020.03.14]
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 薄荷Eβf合成酶基因(Tspa11)的克隆、序列分析与表达特性()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第35卷
期数:
2020年第3期
页码:
90-94
栏目:
出版日期:
2020-05-31

文章信息/Info

Title:
 Effect of Storage at Normal Temperature on the Quality of Zanthoxylum bungeanum
文章编号:
1001-7461(2020)03-0090-05
作者:
 刘一钒王丹丹*李晓红
 (辽东学院 农学院,辽宁 丹东 118003)
Author(s):
 LIU Yi-fanWANG Dan-dan*LI Xiao-hong
 (College of Agriculture,Eastern Liaoning University,Dandong 118003,Liaoning,China)
关键词:
 薄荷Eβf合成酶基因抗蚜基因生物信息学分析聚类分析
Keywords:
Mentha haplocalyx Eβf synthetase gene aphid resistance gene bioinformatics analysis cluster analysis
分类号:
S567.235
DOI:
10.3969/j.issn.1001-7461.2020.03.14
文献标志码:
A
摘要:
 采用室温贮藏的方法,研究贮藏时间对大红袍花椒和青花椒品质性状的影响。结果表明,贮藏1、2、3 a时,大红袍花椒果皮麻味素含量和脂肪酸含量降低,分别较对照(当年)降低14.4%、50.8%、69.7.0%和2.0%、10.0%、14.0%,青花椒分别降低11.0%、40.5%、44.2%和6.5%、21.7%、22.8%。种子贮藏1、2、3 a时,大红袍花椒和青花椒脂肪酸含量分别较对照(当年)降低了0.98%、19.6%、20.2%和2.2%、15.3%、22.4%。同时,脂肪酸组分含量受贮藏时间的影响显著。贮藏1 a时,大红袍花椒和青花椒果皮亚麻酸含量分别较对照提高19.2%和26.9%(P<0.1),贮藏3 a时,分别减少27.6%和35.8%(P<0.1),亚油酸含量分别减少22.2%和15.7%。贮藏1 a的花椒果皮氨基酸含量显著提高,但随时间延长,其含量降低。同时,贮藏1 a时,大红袍花椒果皮中天冬氨酸、脯氨酸、胱氨酸和精氨酸含量分别较对照提高45.2%、55.4%、66.7%、128.6%(P<0.1),青花椒果皮中天冬氨酸、谷氨酸、脯氨酸含量分别较对照提高40.8%、30.7%和60.9%(P<0.1)。室温条件下,花椒保存时间以1 a以内为宜。
Abstract:
 The objectives of this study were to clone the Tspa11 gene of Mentha haplocalyx,to analyze the structure,physical and chemical properties of the protein,and to determine the differences of expression in different tissues and different developmental stages of the worker bees.Tissues of M.haplocalyx were used as materials.The cDNA sequence of odorant receptor gene Tspall was obtained by RT-PCR.The structural properties of encoded protein were analyzed by the bioinformatics software,and the phylogenetic tree of Tspa11 based on amino acid was constructed by MMEGA7.0 neighbor-joining method.The different expression levels of Tspa11 gene in different tissues (seeds,roots,stems,leaves,and flowers) were detected by quantitative real-time PCR at different developmental stages.The results showed that the length of Tspa11 gene was 1 929 bp,encoding a putative protein of 550 amino acids,with an estimated molecular weight 63.74 ku,the theoretical pI 5.21,indicating it was a acidic and stable hydrophobic protein.The N-terminal was intracellularly located (a conserved Isoprenoid_Biosyn_C1 superfamily domain); the amino acid homology analysis showed that the amino acid sequence of Eβf synthetase was consistent with that of Prunella vulgaris’s gene with the similarity of as high as 90%.The expression of Tspa11 in seeds was significantly higher than that in other tissues at the same stage (P<0.01),and the expression level in root was lowest.

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备注/Memo

备注/Memo:
 收稿日期:2019-09-20修回日期:2019-12-26
基金项目:中央财政专项:生物产业技术研发平台——满族道地药材种植基地建设(851800501);丹东市2016科技攻关(2016KJ001);辽东学院青年基金(20175QN050);辽宁省省级大学生创新项目。
作者简介:刘一钒。研究方向:分子生物学。E-mail:1182047108@qq.com
*通信作者:王丹丹,硕士,副教授。研究方向:分子生物学。E-mail:wangdandansq@sina.com
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