[1]杨晨星,李晶,穆立蔷*. 不同消毒方法对组织培养中大岩桐叶片生长状况的影响[J].西北林学院学报,2020,35(4):89-94.[doi:10.3969/j.issn.1001-7461.2020.04.15]
　YANG Chen-xing,LI Jing,MU Li-qiang*. Effects of Different Disinfection Methods on the Leaf Growth of Sinningia speciosa in Tissue Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2020,35(4):89-94.[doi:10.3969/j.issn.1001-7461.2020.04.15]
点击复制

 不同消毒方法对组织培养中大岩桐叶片生长状况的影响()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第35卷
期数:
2020年第4期
页码:
89-94
栏目:
出版日期:
2020-09-29

文章信息/Info

Title:
 Effects of Different Disinfection Methods on the Leaf Growth of Sinningia speciosa in Tissue Culture
文章编号:
1001-7461(2020)04-0089-06
作者:
 杨晨星1; 李晶2; 穆立蔷1*
 (1.东北林业大学 林学院，黑龙江 哈尔滨 150040；2.黑龙江省林业科学院，黑龙江 哈尔滨 150040)
Author(s):
 YANG Chen-xing1; LI Jing2; MU Li-qiang1*
 (1.Forestry College,Northeast Forestry University,Harbin 150040,Heilongjiang,China; 2.Heilongjiang Academy of Forestry Science,Harbin 150040,Heilongjiang,China)
关键词:
 ; 大岩桐; 叶片; 组织培养; 消毒时间; 消毒剂种类
Keywords:
Sinningia speciose;  leaf;  tissue culture;  sterilization time;  disinfectant species
分类号:
S723.13
DOI:
10.3969/j.issn.1001-7461.2020.04.15
文献标志码:
A
摘要:
 为优选出大岩桐组织培养中最佳的消毒灭菌方法，以大岩桐幼嫩叶片为试验材料，研究前期75％酒精（10、30、60 s）预处理以及后期0.1% HgCl2、5% NaClO、10% H2O2 3种不同消毒剂种类分别在（8、10、12 min）处理下对大岩桐生长状况的影响。结果表明，10％ H2O2处理下的长菌率和褐化率显著高于0.1% HgCl2和5% NaClO的处理，且长菌和褐化发生时间较早；研究中消毒效果最佳的2个组合为75％酒精处理30 s+0.1% HgCl2处理10 min和75％酒精处理30 s+5% NaClO处理10 min。该研究为与大岩桐类似的其他被子植物的组织培养提供了参考。
Abstract:
 In order to optimize the sterilization method in tissue culture of Sinningia speciosa,young leaves of S.speciosa were used as experimental materials.The effects of pretreatment with 75% alcohol (10,30,and 60 s) in the early stage and post-treatment with 0.1% HgCl2,5% NaClO and 10% H2O2 (8,10,and 12 min each) on the growth of S.speciosa leaves were studied.The results showed that the pollution rate and browning rate under 10% H2O2 treatment were significantly higher than those of 0.1% HgCl2 and 5% NaClO,and the pollution and browning occurred earlier.The two most effective combinations were 75% alcohol treatment for 30 s+0.1% HgCl2 for 10 min and 75% alcohol treatment for 30 s+5% NaClO for 10 min.This experiment provides a reference for tissue culture of other angiosperms similar to globulus.

相似文献/References:

[1]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
　LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(4):21.
[2]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
　ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(4):38.
[3]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
　YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(4):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[4]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
　JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(4):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[5]牛晓霆,王逢瑚,曹新民. 明清家具匠师原木下料的工艺原则及措施[J].西北林学院学报,2013,28(01):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
　NIU Xiao-ting,WANG Feng-hu,CAO Xin-min. Techniques and Principles of Log Cuttings of Furniture Makers in Ming and Qing Dynasties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(4):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
[6]吕金阳,覃卓凯,舒辉,等. 银杉木材构造美学价值[J].西北林学院学报,2013,28(01):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
　LV Jin-yang,QIN Zhuo-kai,SHU Hui,et al. Esthetical Values in Wood Structure of Cathaya argyrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(4):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
[7]杨艳红,张海敏,孙景荣. 基于传统文化视角的现代家具设计探析[J].西北林学院学报,2013,28(01):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
　YANG Yan-hong,ZHANG Hai-min,SUN Jing-rong. Study of Modern Furniture Design Based on Traditional Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(4):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
[8]黄圣游,叶喜. 新中式家具“中式”传统的界定[J].西北林学院学报,2013,28(01):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
　HUANG Sheng-You,YE Xi. Definition of Traditional Element in New Chinese Style Furniture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(4):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
[9]韩维生,赵明磊,王宏斌. 基于阈限理论的设计尺度体系[J].西北林学院学报,2013,28(01):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
　HAN Wei-sheng,ZHAO Ming-lei,WANG Hong-bin. Design Scale System Based on Threshold Theory[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(4):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
[10]宋杰,侯建军,申黎明. 按摩椅按摩头包覆层对按摩舒适性的影响[J].西北林学院学报,2013,28(01):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
　SONG Jie,HOU Jian-jun,SHEN Li-ming. Influence of Massager Coat Layer of Massage Chairs on the Degree of Comfort[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(4):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]

备注/Memo

备注/Memo:
 收稿日期：2019-10-15修回日期：2019-12-04
基金项目：黑龙江省林业外来物种调查与研究（KJZXSA2019031）。
作者简介：杨晨星。研究方向：资源植物保护与利用。E-mail:yanchenxing123@foxmail.com
*通信作者：穆立蔷，教授，博士。研究方向：树木学及资源植物保护与利用。E-mail:mlq0417@163.com
更新日期/Last Update: