[1]樊梦颖,张明明,张情,等. 不同月份沙棘根瘤细菌群落结构特征的分析对比[J].西北林学院学报,2020,35(6):160-167.[doi:10.3969/j.issn.1001-7461.2020.06.23]
　FAN Meng-ying,ZHANG Ming-ming,ZHANG Qing,et al. Comparison of the Characteristics of Bacterial Community in Root Nodules of Hippophae rhamnoides in Different Months[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2020,35(6):160-167.[doi:10.3969/j.issn.1001-7461.2020.06.23]
点击复制

 不同月份沙棘根瘤细菌群落结构特征的分析对比()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第35卷
期数:
2020年第6期
页码:
160-167
栏目:
出版日期:
2020-11-30

文章信息/Info

Title:
 Comparison of the Characteristics of Bacterial Community in Root Nodules of Hippophae rhamnoides in Different Months
文章编号:
1001-7461(2020)06-0160-08
作者:
 樊梦颖; 张明明; 张情; 张春丽; 刘西平*
 （西北农林科技大学 生命科学学院，陕西 杨陵 712100）
Author(s):
 FAN Meng-ying; ZHANG Ming-ming; ZHANG Qing; ZHANG Chun-li; LIU Xi-ping*
 (College of Life Science,Northwest A&F University,Yangling 712100,Shaanxi,China)
关键词:
 ; 沙棘; 根瘤; 高通量测序; 弗兰克氏菌; 细菌群落结构
Keywords:
Hippophae rhamnoides;  nodule;  high-throughput sequencing;  Frankia;  bacterial community structure
分类号:
S763.13
DOI:
10.3969/j.issn.1001-7461.2020.06.23
文献标志码:
A
摘要:
 为深入了解沙棘（Hippophae rhamnoides）根瘤细菌的多样性及其随时间变化，采用高通量测序技术对中国沙棘（H.rhamnoides subsp. sinensis）4个不同月份根瘤细菌群落结构特征进行了分析对比。结果表明，在根瘤瘤瓣刚刚开始萌发的3月，沙棘根瘤细菌的多样性和丰富度显著高于5、7月和8月。4个采样时间所获得的根瘤细菌可归类为21门36纲75目145科234属，其中有8菌门的17菌纲在不同采样时间一直存在。从相对丰度来看，放线菌门放线菌纲（Actinobacteria）始终为优势菌纲，其相对丰度3月为65.30%，5、7月和8月均超过96.05%；同时，根瘤细菌中还存在变形菌门的α-、β-和γ-变形菌纲（Alpha-,Beta-,Gammaproteobacteria）以及拟杆菌门噬纤维菌纲（Cytophagia）和鞘脂杆菌纲（Sphingobacteriia）等多种类型的非放线菌。弗兰克氏菌目（Frankiales）细菌在整体根瘤细菌群落中的相对丰度3月为48.60%，显著低于5月的93.54%、7月的95.48%和8月的94.73%；4个采样时间共获得5科6属10条可操作分类单元（operational taxonomic units，OTUs），其中弗兰克氏菌科（Frankiaceae）弗兰克氏菌属（Frankia）的OTU235占该目的相对丰度在不同时间均在99%。表明沙棘根瘤细菌群落在不同月份均是极其丰富和多样的，其中既有多种类型的放线菌也有多种类型的非放线菌，但均以弗兰克氏菌科弗兰克氏菌属细菌占绝对优势；同时，根瘤细菌群落结构（特别是弗兰克氏菌的相对丰度）随季节发生着显著变化。
Abstract:
 In order to deeply understand the diversity and time variations of Hippophae rhamnoides root nodule bacteria (RNB),the characteristics of bacterial community of the nodules of H.rhamnoides subsp.sinensis in four different months were analyzed and compared by using high-throughput sequencing technology.The results showed that the diversity and the richness of the RNB in March when the new root nodules just germinated were significantly higher than those in May,July and August.The bacteria obtained from the nodules in four sampling time could be classified into 21 phyla,36 classes,75 orders,145 families,and 234 genera.Among them,17 classes of 8 phyla were found all the time.From the view of relative abundance,Actinobacteria was always the dominant class,65.30% in March,and over 96.05% in May,July and August.Many types of non-actinomycetes,such as the Alpha-,Beta-and Gamma-proteobacteria of the phylum Proteobacteria as well as Cytophagia and Sphingobacteriia of the phylum Bacteroidetes also presented.In March,the relative abundance of Frankiales in the whole bacterial community was 48.60%,which was significantly lower than those in May (93.54%),July (95.48%) and August (94.73%).A total of 10 operational taxonomic units (OTUs) belonging to 6 genera of 5 families of Frankiales were obtained,in which OTU235 belonging to the genus Frankia always remained a relative abundance of about 99% in all of the sampling time.In summary,the community of H.rhamnoides RNB in different months was extremely rich and diverse,including many types of actinomycetes and non-actinomycetes.Frankia always was the dominate genus.And the bacterial community structure (especially the relative abundance of Frankia) of nodules changed significantly with seasons.

相似文献/References:

[1]李子敬,袁玉欣＊,刘炳响,等.铁尾矿区沙棘林生物量和生产力的研究[J].西北林学院学报,2008,23(03):16.
　LI Zijing,YUAN Yuxi n,LIU Bingxiang,et al.Studies on the Biomass and Productivity of Hippophae rhamnoide Forest in Iron Tailing Wast eland[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(6):16.
[2]张端伟,宋西德,张宗勤,等.沙棘叶片不定芽再生的研究[J].西北林学院学报,2008,23(04):93.
　ZHANG Duan wei,SONG Xi de*,ZHANG Zong qin,et al.Studies on the Adventitious Shoot Regeneration from the Leaves of Hippophae rhamnoides L.[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(6):93.
[3]叶存旺,翟巧绒,郭梓娟,等.沙棘-侧柏混交林土壤养分、微生物与酶活性的研究[J].西北林学院学报,2007,22(05):1.
　YE Cun-wang,ZHAI Qiao-rong,GUO Zi-juan,et al.Soil Nutrient, Microorganism and Enzyme Activity of Hippophae rhamnoides and Platycladus orientalis Mixed Forests[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(6):1.
[4]马明呈,宋西德*,桓国江,等.青海贵南高海拔沙地沙棘种群根系生物量的研究[J].西北林学院学报,2006,21(03):11.
　MA Mingcheng,SONG Xide,HUAN Guojiang,et al.Studies on Roots Biomass of Hippophae rhamnoides Community in High Altitude Sand in Guinan of Qinghai[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2006,21(6):11.
[5]宋于洋,塔依尔,王炳举,等.沙棘叶片光合速率与其环境因子的日变化规律研究沙棘叶片光合速率与其环境因子的日变化规律研究[J].西北林学院学报,2007,22(01):8.
　SONG Yuyang,Tayir,WANG Bingju,et al.Daily Changing Rule between Leaf Net Photosynthetic Rate and Plant Environment Factors in Hippophae rhamnoides L.[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(6):8.
[6]韩恩贤,韩刚,薄颖生.黄土高原油松、侧柏与沙棘人工混交林生长及土壤特性研究[J].西北林学院学报,2007,22(03):100.
　HAN Enxian,HAN Gang,BO Yingsheng.Studies on the Growth and Soil Properties of Pinus tabulaeformis, Platycladus orientalis and Hippophae rhamnoides Mixed Plantation on Loess Plateau[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(6):100.
[7]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
　LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(6):21.
[8]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
　ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(6):38.
[9]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
　YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[10]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
　JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[11]任珺,陶玲,赵迎迎,等. 播种深度对青藏铁路沙区植物白刺、油蒿、沙棘存活和生长的影响[J].西北林学院学报,2012,27(02):13.
　REN Jun,TAO Ling,ZHAO Ying-ying,et al. Influences of Sowing Depth on Survival and Growth of Nitraria tangutorum, Hippophae rhamnoides and Artemisia ordosica in Sandy Lands Along QinghaiTibet Railway[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(6):13.
[12]安宝利,张征,王举位,等. 基于AHP-模糊综合评价法的沙棘涵养水源功能评价[J].西北林学院学报,2012,27(02):39.
　AN Bao-li,ZHANG Zheng,WANG Ju-wei,et al. Assessment of Water Conservation Function of Seabuckthorn Based on AHP-Fuzzy Comprehensive Theory[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(6):39.
[13]姚景瀚,李伟. 沙棘微扦插快繁技术的应用研究[J].西北林学院学报,2013,28(02):109.[doi:10.3969/j.issn.1001-7461.2013.02.21]
　YAO Jing-han,LI Wei. Applications of Micro-cuttage on the Rapid Propagation of Sea-Buckthorn[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):109.[doi:10.3969/j.issn.1001-7461.2013.02.21]
[14]张鹏,何梦雅,张宇,等. 温度与浸种处理对沙棘种子萌发的影响[J].西北林学院学报,2015,30(06):130.[doi:doi:10.3969/j.issn.1001-7461.2015.06.23]
　ZHANG Peng,HE Meng-ya,ZHANG Yu,et al. Effects of Temperature and Soaking Treatments on Seed Germination of Hippophae rhamnoides[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(6):130.[doi:doi:10.3969/j.issn.1001-7461.2015.06.23]
[15]艾宁,强大宏,刘广全*,等. 煤矿复垦区林龄与叶位对沙棘果叶含水量的影响[J].西北林学院学报,2020,35(1):68.[doi:10.3969/j.issn.1001-7461.2020.01.10]
　AI Ning,QIANG Da-hong,LIU Guang-quan*,et al. Effects of Forest Age and Leaf Position on the Water Content of the Fruit and Leaf of Hippophae rhamnoides in Coal Mine Reclamation Area[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2020,35(6):68.[doi:10.3969/j.issn.1001-7461.2020.01.10]
[16]张情,张明明,樊梦颖,等. 沙棘根瘤及根瘤内弗兰克氏菌形态的显微观察[J].西北林学院学报,2020,35(1):165.[doi:10.3969/j.issn.1001-7461.2020.01.25]
　ZHANG Qing,ZHANG Ming-ming,FAN Meng-ying,et al. Microscopic Observation of the Root Nodule and the Morphology of Frankia in Root Nodules of Hippophae rhamnoides[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2020,35(6):165.[doi:10.3969/j.issn.1001-7461.2020.01.25]
[17]张明明,张情,樊梦颖,等. 2种立地条件下沙棘林地土壤中细菌多样性比较[J].西北林学院学报,2020,35(2):32.[doi:10.3969/j.issn.1001-7461.2020.02.05]
　ZHANG Ming-ming,ZHANG Qing,FAN Meng-ying,et al. Comparison of Bacterial Diversity in Forest Soils of Hippophae rhamnoides Growing in Two Different Sites[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2020,35(6):32.[doi:10.3969/j.issn.1001-7461.2020.02.05]
[18]陈玉鑫,龚悦,杨文涛,等. 14种杀虫剂对栎黄枯叶蛾4龄幼虫的室内生物活性[J].西北林学院学报,2022,37(2):170.[doi:10.3969/j.issn.1001-7461.2022.02.23]
　CHEN Yu-xin,GONG Yue,YANG Wen-tao,et al. Bioactivity of 14 Insecticides Against the 4th Instar Larvae of Trabala vishnou gigantina[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2022,37(6):170.[doi:10.3969/j.issn.1001-7461.2022.02.23]
[19]刘青青,马玉花*,董佳伟,等. 干旱胁迫下沙棘CMO基因的时空表达模式与蛋白结构预测[J].西北林学院学报,2024,39(2):21.[doi:10.3969/j.issn.1001-7461.2024.02.03]
　LIU Qing-qing,MA Yu-hua*,DONG Jia-wei,et al. Spatiotemporal Expression Patterns and Protein Structure Prediction of Seabuckthorn CMO Gene Under Drought Stress[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2024,39(6):21.[doi:10.3969/j.issn.1001-7461.2024.02.03]

备注/Memo

备注/Memo:
 收稿日期：2020-01-10修回日期：2020-05-08
作者简介：樊梦颖，硕士在读。研究方向：根瘤微生物。E-mail：myingfan@163.com
*通信作者：刘西平，教授，博士生导师。研究方向：植物逆境生理。E-mail：xpliuderen@163.com
更新日期/Last Update: