[1]张宝,陈冰炜,翟胜丞*,等. 不同染料标识木栓细胞壁中木栓脂的分布[J].西北林学院学报,2021,36(2):208-212.[doi:10.3969/j.issn.1001-7461.2021.02.31]
　ZHANG Bao,CHEN Bing-wei,ZHAI Sheng-cheng*,et al. Distribution of Suberin in the Phellem Cells Determined by Different Dyes[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2021,36(2):208-212.[doi:10.3969/j.issn.1001-7461.2021.02.31]
点击复制

 不同染料标识木栓细胞壁中木栓脂的分布()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第36卷
期数:
2021年第2期
页码:
208-212
栏目:
出版日期:
2021-03-31

文章信息/Info

Title:
 Distribution of Suberin in the Phellem Cells Determined by Different Dyes
文章编号:
1001-7461(2021)02-0208-05
作者:
 张宝1; 陈冰炜1; 翟胜丞1; 2*; 潘彪1; 魏建强1; 勇强2; 3
 （1.南京林业大学 材料科学与工程学院，江苏 南京 210037；2.南京林业大学 林业资源高效加工利用协同创新中心，江苏 南京 210037；3.南京林业大学 化学工程学院，江苏 南京 210037)
Author(s):
 ZHANG Bao1; CHEN Bing-wei1; ZHAI Sheng-cheng1; 2*; PAN Biao1; WEI Jian-qiang1; YONG Qiang2; 3
 (1.College of Materials Science and Engineering Nanjing Forestry University,Nanjing 210037,Jiangsu,China；2.Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing Forestry University,Nanjing 210037,Jiangsu,China; 3.College of Chemical Engineering,Nanjing Forestry University,Nanjing 210037,Jiangsu,China)
关键词:
 ; 日本晚樱; 木栓脂; 染料; 荧光; 分布
Keywords:
Cerasus serrulata;  suberin;  dye;  fluorescence;  distribution
分类号:
S781
DOI:
10.3969/j.issn.1001-7461.2021.02.31
文献标志码:
A
摘要:
 以日本晚樱（Cerasus serrulata）为试验对象，取其新鲜树皮经固定、脱水、不同树脂包埋、切片，使用甲苯胺蓝、盐酸-间苯三酚、苏丹Ⅲ、苏丹红7B、硫酸氢黄连素-苯胺蓝5种染色对切片进行染色，研究木栓细胞中木栓脂的分布。结果表明：1）经不同染料染色后在明场下观察，苏丹Ⅲ的染色效果最好，可直接将木栓脂染成红色。结合甲苯胺蓝、苏丹红7B和盐酸-间苯三酚染色观察，均可确定木栓脂主要存在于木栓细胞壁的最外层。2）甲苯胺蓝可抑制木质素自发荧光，避免木质素自发荧光的干扰，直接观察到木栓脂的荧光。3）硫酸氢黄连素-苯胺蓝染色后，木栓脂的荧光在木栓细胞壁外侧呈灰绿色，木质素的荧光在木栓细胞壁的内侧呈黄绿色。4）盐酸-间苯三酚染色后，木栓细胞壁内侧富含木质素的壁层被染为红棕色，且环氧树脂包埋会降低盐酸-间苯三酚的染色效果。
Abstract:
 In this paper,the fresh bark of Cerasus serrulata was collected,fixed with FAA solution,dehydrated,resin embedded and sliced.Then the sections were stained with toluidine blue,hydrochloric acid-resorcinol,Sudan III,Sudan red 7B,Berberine hemisulfate salt-aniline blue to study the distribution of suberin in phellems cell wall.The results showed that 1) after dyed and observed in bright field,Sudan III had the best dyeing effect,and it could directly dye the suberin red.Simultaneously,toluidine blue O,Sudan red 7B and hydrochloric acid-resorcinol staining confirmed that the suberin was mainly present in the outermost layer of the phellem cell wall.2) Toluidine blue O could inhibit the auto-fluorescence of lignin,avoid the interference of the auto-fluorescence of lignin,and directly observe the fluorescence of suberin.3) After berberine bisulfate-aniline blue stained,the fluorescence of the suberin was gray-green outside the phellem cell wall,and the fluorescence of lignin was yellow-green on the inside of the phellem cell wall.4) After hydrochloric acid-resorcinol stained,the lignin inside the phellem cell wall appeared reddish brown,and the epoxy resin embedding could reduce the staining effect of hydrochloric acid-resorcinol.

相似文献/References:

[1]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
　LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(2):21.
[2]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
　ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(2):38.
[3]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
　YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[4]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
　JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[5]牛晓霆,王逢瑚,曹新民. 明清家具匠师原木下料的工艺原则及措施[J].西北林学院学报,2013,28(01):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
　NIU Xiao-ting,WANG Feng-hu,CAO Xin-min. Techniques and Principles of Log Cuttings of Furniture Makers in Ming and Qing Dynasties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
[6]吕金阳,覃卓凯,舒辉,等. 银杉木材构造美学价值[J].西北林学院学报,2013,28(01):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
　LV Jin-yang,QIN Zhuo-kai,SHU Hui,et al. Esthetical Values in Wood Structure of Cathaya argyrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
[7]杨艳红,张海敏,孙景荣. 基于传统文化视角的现代家具设计探析[J].西北林学院学报,2013,28(01):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
　YANG Yan-hong,ZHANG Hai-min,SUN Jing-rong. Study of Modern Furniture Design Based on Traditional Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
[8]黄圣游,叶喜. 新中式家具“中式”传统的界定[J].西北林学院学报,2013,28(01):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
　HUANG Sheng-You,YE Xi. Definition of Traditional Element in New Chinese Style Furniture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
[9]韩维生,赵明磊,王宏斌. 基于阈限理论的设计尺度体系[J].西北林学院学报,2013,28(01):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
　HAN Wei-sheng,ZHAO Ming-lei,WANG Hong-bin. Design Scale System Based on Threshold Theory[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
[10]宋杰,侯建军,申黎明. 按摩椅按摩头包覆层对按摩舒适性的影响[J].西北林学院学报,2013,28(01):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
　SONG Jie,HOU Jian-jun,SHEN Li-ming. Influence of Massager Coat Layer of Massage Chairs on the Degree of Comfort[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]

备注/Memo

备注/Memo:
 收稿日期：2020-06-17修回日期：2020-06-29
基金项目：国家自然科学基金（31400496）；江苏省自然科学基金（BK20140981）。
作者简介：张宝，硕士在读。研究方向：木材形成与细胞壁超微构造。E-mail:njfuzhangb@163.com
*通信作者：翟胜丞，副教授，博士。研究方向：木材解剖学、有机质文物保存与保护。E-mail：zhais@njfu.edu.cn
更新日期/Last Update: