[1]朱立华#,刘召阳#,刘维,等. 苹果树腐烂病菌Zn(Ⅱ)2Cys6类转录因子Vmzctf-07的鉴定及其功能分析[J].西北林学院学报,2022,37(4):188-195.[doi:10.3969/j.issn.1001-7461.2022.04.25]
　ZHU Li-hua#,LIU Zhao-yang#,LIU Wei,et al. Identification and Functional Analysis of Vmzctf-07,A Zn(Ⅱ)2Cys6 Transcription Factor from Valsa mali[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2022,37(4):188-195.[doi:10.3969/j.issn.1001-7461.2022.04.25]
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 苹果树腐烂病菌Zn(Ⅱ)2Cys6类转录因子Vmzctf-07的鉴定及其功能分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第37卷
期数:
2022年第4期
页码:
188-195
栏目:
出版日期:
2022-07-31

文章信息/Info

Title:
 Identification and Functional Analysis of Vmzctf-07,A Zn(Ⅱ)2Cys6 Transcription Factor from Valsa mali
文章编号:
1001-7461(2022)04-0188-08
作者:
 朱立华#; 刘召阳#; 刘维; 张贤玉; 黄丽丽*; 冯浩*
 （西北农林科技大学 植物保护学院 旱区作物逆境生物学国家重点实验室，陕西 杨陵 712100）
Author(s):
 ZHU Li-hua#; LIU Zhao-yang#; LIU Wei; ZHANG Xian-yu; HUANG Li-li*; FENG Hao*
 (State Key Laboratory of Crop Stress Biology for Arid Areas,College of Plant Protection,Northwest A&F University,Yangling 712100,Shaanxi,China)
关键词:
 ; 苹果树黑腐皮壳菌; 转录因子; 基因敲除; 生物学特征
Keywords:
Valsa mali Miyabe et Yamada;  transcription factor;  gene knockout;  biological characteristics
分类号:
S763.1
DOI:
10.3969/j.issn.1001-7461.2022.04.25
文献标志码:
A
摘要:
 转录因子是真菌生命过程中重要的调控因子，探究转录因子在苹果树腐烂病菌Valsa mali致病过程中的作用，能够为解析病菌致病机理奠定基础。通过构建Vmzctf-07基因的酵母单杂交和亚细胞定位表达载体，进行转录激活域验证和亚细胞定位分析；通过创制Vmzctf-07基因缺失突变体和回补菌株，进行生长速率、非生物胁迫和致病力测定。结果表明，Vmzctf-07具有转录激活功能，转录激活域可能存在于down区段（aa 1 455~aa 1 644）。Vmzctf-07定位于细胞核中，符合转录因子典型特征。Vmzctf-07敲除突变体的生长速率和致病力均显著下降，同时该基因参与病菌响应渗透胁迫和氧化胁迫。综上，Vmzctf-07具有转录激活功能和核定位信号，且参与调节V.mali的生长发育、非生物胁迫和致病过程。
Abstract:
 Transcription factors (TFs) are important regulators in the life process of fungi.Exploring the role of TFs in the pathogenic process of Valsa mali can lay the foundation for the analysis of pathogenic mechanisms of pathogens.The yeast one-hybrid and subcellular localization expression vectors of Vmzctf-07 were constructed to verify the transcriptional activation domain and analyze the subcellular localization,respectively.The Vmzctf-07 knockout mutants and complement strains were constructed to test the growth rate,response to abiotic stresses and pathogenicity.The results showed that the transcriptional activation domain of Vmzctf-07 may exist in the down segment (aa 1 455-aa 1 644).Vmzctf-07 was located in nuclear,which accorded with TFs’ characteristics.The growth rate and pathogenicity of the knockout mutants were significantly reduced.Meanwhile,Vmzctf-07 was found to be involved in the response to osmotic stress and oxidative stress of V.mali.In summary,Vmzctf-07 has transcriptional activation function and is located in nuclear,and it is involved in regulating the growth,response to abiotic stress and pathogenic process of V.mali.

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备注/Memo

备注/Memo:
 收稿日期：2021-06-21修回日期：2021-10-28
基金项目：陕西省创新人才推进计划——青年科技新星项目（2021KJXX-10）；陕西省普通高校青年杰出人才支持计划。
第一作者：朱立华。研究方向：植物病理学。E-mail：18233260015@163.com；刘召阳。研究方向：植物病理学。E-mali：1211086358@qq.com
*通信作者：冯浩，教授。研究方向：苹果重大病害致病机理及综合防控。E-mali：xiaosong04005@163.com；黄丽丽，教授。研究方向：果树病害病原生物学及综合防控。E-mali：huanglili@nwsuaf.edu.cn
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