[1]武文雅,吴可心,张迈,等. 基于GC-MS的3种龙胆属植物的代谢差异分析[J].西北林学院学报,2023,38(6):203-210.[doi:10.3969/j.issn.1001-7461.2023.06.27]
　WU Wen-ya,WU Ke-xin,ZHANG Mai,et al. Metabolic Difference Analysis of Three Gentiana Species Based on GC-MS[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2023,38(6):203-210.[doi:10.3969/j.issn.1001-7461.2023.06.27]
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 基于GC-MS的3种龙胆属植物的代谢差异分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第38卷
期数:
2023年第6期
页码:
203-210
栏目:
出版日期:
2023-11-30

文章信息/Info

Title:
 Metabolic Difference Analysis of Three Gentiana Species Based on GC-MS
文章编号:
1001-7461(2023)06-0203-08
作者:
 武文雅; 吴可心; 张迈; 穆立蔷*
 (东北林业大学 林学院，黑龙江 哈尔滨 150040）
Author(s):
 WU Wen-ya; WU Ke-xin; ZHANG Mai; MU Li-qiang*
 (College Forestry,Northeast Forestry University,Harbin 150040,Heilongjiang,China)
关键词:
 ; 龙胆; 三花龙胆; 条叶龙胆; 代谢组学; GC-MS
Keywords:
Gentiana scabra;  Gentiana triflora;  Gentiana manshurica;  metabonomics;  GC-MS
分类号:
Q949.776
DOI:
10.3969/j.issn.1001-7461.2023.06.27
文献标志码:
A
摘要:
 为明确中药龙胆的可入药植物即龙胆、三花龙胆及条叶龙胆根部活性成分的差异及3种龙胆的叶片是否具有开发利用价值，通过气相色谱－质谱联用（GC-MS）技术进行相关代谢物数据采集，根据非靶向代谢组学对3种龙胆根部及叶片的化学组成成分进行研究。采用无监督模式的主成分分析（PCA）、单变量与多元统计分析相结合的偏最小二乘法（PLS-DA）分析和差异代谢物相对含量的聚类分析。结果表明，PCA可对3种龙胆根部及3种龙胆叶片进行明显的区分；PLS-DA分析及聚类分析结果显示在龙胆、三花龙胆、条叶龙胆根部发现了58种差异代谢物，叶片中发现了46种差异代谢物，其中大部分代谢物在龙胆中的积累量高于三花龙胆及条叶龙胆。通过KEGG富集通路分析发现L-脯氨酸和L-丙氨酸均参与了ABC转运蛋白途径，且这2种代谢物在龙胆根部及叶片中均表现为上调，龙胆在抗逆性能及抵御外界病菌能力上优于三花龙胆及条叶龙胆，因此累积下来的化学成分含量也高于三花龙胆及条叶龙胆。研究结果为中药龙胆药原植物的开发利用提供了理论依据。
Abstract:
 In order to understand the difference of active components in the roots of the medicinal plants of three Gentiana species,including G.scabra,G.triflora and G.manshurica,and whether the leaves of the three have medicinal value of development and utilization,this study collected the related metabolite data through gas chromatography-mass spectrometry (GC-MS) and studied the chemical components of the roots and leaves of the three based on non-targeted metabonomics.The unsupervised principal component analysis (PCA),partial least squares analysis (PLS-DA) combined with univariate and multivariate statistical analysis,and cluster analysis of relative content of differential metabolites were used.The results showed that PCA could distinguish the roots and leaves of three Gentiana species; by PLS-DA analysis and cluster analysis,58 different metabolites were found in the roots of Gentiana species,and 46 different metabolites were found in the leaves,of which most of the metabolites accumulated in G.scabra were higher than those in G.triflora and G.manshurica.Through the analysis of KEGG enrichment pathway,it was found that L-proline and L-alanine were involved in the ABC transporter pathway,and these two metabolites were up-regulated in the roots and leaves of G.scabra.G.scabra was superior to G.triflora and G.manshurica in terms of stress resistance and resistance to external bacteria,so the content of chemical components accumulated was also higher than G.triflora and G.manshurica.This study provides a theoretical basis for the development and utilization of the original plant of G.scabra.

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备注/Memo

备注/Memo:
 收稿日期：2022-09-26修回日期：2022-11-16
基金项目：国家科技基础性资源专项（2019FY100505）。
第一作者：武文雅。研究方向：森林植物资源学。E-mail：1806334106@qq.com
*通信作者：穆立蔷，博士生导师，教授。研究方向：森林植物资源学。E-mail：mlq0419@163.com
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