[1]张 翔,刘思源,徐小军,等.五种林木蚜虫的RAPD分析[J].西北林学院学报,2006,21(01):105-106.
　ZHANG Xiang,LIU Siyuan,XU Xiaojun,et al.RAPD Analysis of Five Forest Aphid Species[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2006,21(01):105-106.
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五种林木蚜虫的RAPD分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第21卷
期数:
2006年01期
页码:
105-106
栏目:
森林资源与保护
出版日期:
2006-01-30

文章信息/Info

Title:
RAPD Analysis of Five Forest Aphid Species
作者:
张 翔1;  刘思源2; 徐小军3;  贺虹4
（1.宁夏陶灵园艺试验场，宁夏 银川751400；2.贵州省林业厅,贵州 贵阳 55001； 3.拉萨市林业局，西藏 拉萨 850000；4. 西北农林科技大学 林学院，陕西 杨陵712100）
Author(s):
ZHANG Xiang1; LIU Siyuan2;  XU Xiaojun3;  HE Hong4
(1.Taoling Horticulture Farm, Yinchuan,Ningxia 751400, China;2.Forestry Department of Guizhou Province,Guiyang Guizhou 550001,China;3.Forestry Bureau of Lasa,Tibet 850000,China；4. College of Forestry,Northwest A F University, Yangling, Shaanxi 712100,
关键词:
蚜虫; RAPD; 分类
Keywords:
aphid;  RAPD;  species identification
分类号:
S763.35
文献标志码:
A
摘要:
对洋槐蚜Aphis robiniae、桃蚜Myzus persicae、女贞卷叶绵蚜Prociphilus ligustrifoliae、中华忍冬圆尾蚜Amphicercidus sinalonicericola和柏大蚜Cinera tujafilina等5种危害林木的蚜虫进行了随机扩增多态性DNA(RAPD)分析。研究结果表明 从6种随机引物中筛选出扩增效果好、多态性明显的3种引物S169、S147和S18 ，这3种引物在5种蚜虫中总共扩增出45个RAPD标记，分子量在238-2 355 bp范围之间，多态频率达100%，利用这3种引物均可单独区分5种蚜虫。第1期张 翔等五种林木蚜虫的RAPD分析
Abstract:
Five aphid species harm to trees including %Aphis robiniae, Myzus persicae, Prociphilus ligustrifolia, Amphicercidus sialonicericola% and %Cinera tujafila% were analyzed by RAPD method with 6 arbitrary primers. The result showed that the clear and repeating bands were amplified by 3 primers such as S169, S147 and S18, and obtained 45 DNA fragments whose molecular weight was 238-2 355 bp. Some of distinctive bands were found in every species and so the five species aphids could be distinguished by 3 arbitrary primers.

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备注/Memo

备注/Memo:
收稿日期 20050316修回日期 20050508作者简介 张　翔（1969），女，宁夏中宁人，农艺师，主要从事果园管理和病虫害防治方面的研究。
更新日期/Last Update: 2010-05-04