[1]陈 艳,李广泽*,曹雪峰,等.RP-HPLC法同时测定沙地柏中3种鬼臼毒素类化合物的含量[J].西北林学院学报,2007,22(02):127-130.
　CHEN Yan,LI Guang-ze*,et al.Quantitative Determination of the Podophyllotoxin Analogues in Sabina vulgaris by RP-HPLC[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(02):127-130.
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RP-HPLC法同时测定沙地柏中3种鬼臼毒素类化合物的含量()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第22卷
期数:
2007年02期
页码:
127-130
栏目:
林产化学
出版日期:
2007-03-30

文章信息/Info

Title:
Quantitative Determination of the Podophyllotoxin Analogues in Sabina vulgaris by RP-HPLC
文章编号:
1001-7461(2007)02-0127-04
作者:
陈 艳1; 2;  李广泽1*;  曹雪峰1; 2;  陈存根2;  张 兴1; 3
(1.西北农林科技大学 无公害农药研究服务中心,陕西 杨陵 712100; 2.西北农林科技大学 资源与环境学院，陕西 杨陵 712100; 3.陕西省生物农药工程技术研究中心，陕西 杨陵 712100)
Author(s):
CHEN Yan1; 2; LI Guang-ze1*; CAO Xue-feng1; CHEN Cun-gen1; ZHANG Xing1; 3
（1.Bioratonal Pesticides Research and Development Center; 2.College of Resource and Environmental Science; Northwest A & F University; 3.Shaanxi Research Center of Biopesticide Engineering and Technology; Yangling; Shaanxi 712100; China）
关键词:
沙地柏;  鬼臼毒素;  脱氧鬼臼毒素;  苦鬼臼毒素;  RP-HPLC
Keywords:
Sabina vulgaris;  podophyllotoxin;  deoxypodophyllotoxin;  picropodophyllotoxin;  RP-HPLC
分类号:
S791.440.8
文献标志码:
A
摘要:
用RP-HPLC法同时测定了沙地柏3种鬼臼类化合物(鬼臼毒素、脱氧鬼臼毒素和苦鬼臼毒素)的含量。采用YM C C18反相柱,甲醇∶水(55∶45)为流动相,紫外检测波长为290 nm,鬼臼毒素和脱氧鬼臼毒素在62.50~1 000.00μg/mL范围内的线性方程分别为:Y=(3.502 4+252.122 1X)×1-0 6,Y=(3.580 5+621.046 8X)×1-0 6;相关系数为r=0.999 9和0.999 2;回收率分别为100.932%(RSD=1.388%)和98.968%(RSD=1.108%);苦鬼臼毒素在6.25~100.00μg/mL范围内的线性方程为:Y=(4.781 8+6369.768 0X)×1-0 6,相关系数r=0.999 6,回收率为100.110%(RSD=1.344%)。沙地柏叶中的鬼臼毒素、脱氧鬼臼毒素和苦鬼臼毒素含量分别为3.894、3.345、0.427 m g/g,茎中的含量分别为2.225、1.829、0.241 m g/g;超临界流体萃取沙地柏叶中的3种化合物含量分别为2.327、2.116、0.191 mg/g,茎中的含量分别为1.784、1.446 、0.108 mg/g。该方法步骤简便，结果准确，分析速度快。
Abstract:
A RP-HPLC method was developed for the simultaneous determination of podophyllotoxin, deoxypodophyllotoxin and picropodophyllotoxin in Sabina vulgaris . The chromatographic system consisted of ODS column and mobile phase of methanol-water(55∶45); flow rate: 1 mL/min; detection wavelength :290 nm linear ranges of podophyllotoxin and deoxypodophyllotoxin were 62.50~1 000.00 μg/mL, the regression equation were Y=(3.502 496+252.122 1X)×10-6 and Y=(3.580 590+621.046 8X) ×10-6 respectively; r were 0.999 9 and 0.999 2; the recovery rate were 100.932% (RSD=1.388%) and 98.986%(RSD=1.108%) respectively. The linear range of picropodophyllotoxin was 6.25~100.00 μg/mL, the regression equation was Y=(4.781 883+6369.768 X) ×10-6,r=0.999 6, recovery rate was 100.110%(RSD=1.344%). The contents of three compounds were 3.894 mg/g,3.345 mg/g and 0.427 mg/g respectively in extracts with Soxhlet extraction from the leaves, and 2.225 mg/g,1.829 mg/g and 0.241 mg/g respectively from the stems. In extracts with SFE-CO2, the content of three compounds were 2.327 mg/g,2.116 mg/g,0.191 mg/g respectively from the leaves, and 1.784 mg/g,1.446 mg/g and 0.108 mg/g respectively from the stems. The method developed is simple, accurate, and rapid.

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备注/Memo

备注/Memo:
收稿日期：2006-05-31 修回日期：2006-07-13
基金项目：国家科技攻关计划“西部开发”重大项目（2004BA901A14) 、国家“863”计划项目（2001AA246016)
作者简介：陈艳（1981-) ，女，山东淄博人，硕士，主要研究方向为森林生态学。E-mail:yanzichen6460@163.com
*通讯作者：李广泽。Tel: 13720556618 E-mail:liguangze@126.com.
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