[1]张端伟,宋西德,张宗勤,等.沙棘叶片不定芽再生的研究[J].西北林学院学报,2008,23(04):93.
　ZHANG Duan wei,SONG Xi de*,ZHANG Zong qin,et al.Studies on the Adventitious Shoot Regeneration from the Leaves of Hippophae rhamnoides L.[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(04):93.
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沙棘叶片不定芽再生的研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第23卷
期数:
2008年04期
页码:
93
栏目:
林木遗传育种
出版日期:
2008-07-30

文章信息/Info

Title:
Studies on the Adventitious Shoot Regeneration from the Leaves of Hippophae rhamnoides L.
作者:
张端伟; 宋西德; 张宗勤; 等
西北农林科技大学 林学院，陕西 杨陵 712100
Author(s):
ZHANG Duanwei;  SONG Xide*;  ZHANG Zongqin;  ZHANGYong;  ZHOU Fengli;  PANJing
College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China
关键词:
沙棘; 叶片; 不定芽; 再生
Keywords:
Hippophae rhamnoides L.; leaf;  adventitious shoot;  regeneration
分类号:
S723.132
文献标志码:
A
摘要:
以"实优1号"沙棘枝条水培芽叶片为试材，研究了叶片着生节位、同一叶片不同部位、不同浓度比值的6BA与IAA对愈伤组织产生和不定芽再生的影响。结果表明，适宜水培叶片愈伤组织产生的外植体材料为水培茎尖上部与中部叶片的基部和中部，适宜诱导不定芽的外植体为下部叶片与中部叶片；同一叶片不同部位对不定芽再生率影响研究表明叶片中部为首选材料；可迅速高效的诱导不定芽发生的培养基为1/2MS+6BA 0.7 mg/L+IAA0.5 mg/L，平均每外植体分化不定芽数达到6.75个。
Abstract:
Using hydroponics stem apexes of cultivar "Shiyou 1" of Hippophae rhamnoides as explants, different locations on the stem apexes, different parts of the of leaves and different concentrations of 6BA with IAA were tested for their optimal effects on inducing callus and adventitious shoot regeneration. The results showed that the middle and proximal parts of the leaves from the upper and middle parts of the stem apexes were easier to induce callus,the upper and the middle parts of leaves were easier to induce adventitious shoot. The middle of the leaves was the most suitable explants to induce adventitious shoot; The medium that could induce adventitious shoots efficiently was 1/2 MS supplemented with + 6BA 0.7 mg/L + IAA 0.5 mg/L with the multiplication coefficient of 6.75.

参考文献/References:

［1］徐虹,梁宗锁.沙棘组织培养技术的研究［J］.西北植物学报,2001,21(2):267272.
［2］周松坤,宋西德,张宗勤,等.近5年沙棘组织培养研究进展［J］.西北林学院学报,2006,21(6):118122.
［3］孙兰英,单金友,王春艳,等.沙棘组织培养培养基筛选试验［J］.沙棘,1998(3):1415.
［4］赵国林等.沙棘的组织培养和植株再生［J］.植物生理学报,1989,25(1):42.
［5］周松坤,宋西德,张宗勤,等.良种沙棘“实优1 号”组织培养研究［J］.西北林学院学报.2006,21(3) :6771.
［6］黄莺,范燕萍,王文先,等.石竹愈伤组织诱导及再生［J］.华南农业大学学报:自然科学版,2003,24(1):：5052.
［7］于丽艳,王志和,周波,等.苹果离体叶片再生体系两步培养法的研究［J］.落叶果树,2005(2):14.
［8］赵政阳,曹晓玲,黄英,等.叶片成熟度对苹果试管苗叶片再生植株的影响［J］.陕西农业科学,1997(1):2021.

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备注/Memo

备注/Memo:
收稿日期：20071029修回日期：20080321 基金项目： 陕西省农业攻关项目（2007K011101）。作者简介：张端伟，女，在读硕士，主要从事林木育苗研究。Email：zhangduanwei125@126.com *通讯作者：宋西德，男，研究员，研究方向为林木种苗繁育理论与技术。
更新日期/Last Update: 2008-07-30