[1]雷亚灵,李周岐.八仙花茎段组织培养技术研究[J].西北林学院学报,2008,23(04):101.
　LEI Ya ling,LI Zhou qi*.Study on Tissue Culture of stem segment of Hydrangea macrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(04):101.
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八仙花茎段组织培养技术研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第23卷
期数:
2008年04期
页码:
101
栏目:
林木遗传育种
出版日期:
2008-07-30

文章信息/Info

Title:
Study on Tissue Culture of stem segment of Hydrangea macrophylla
作者:
雷亚灵; 李周岐
西北农林科技大学 林学院，陕西 杨陵712100
Author(s):
LEI Yaling; LI Zhouqi*
College of Forestry, Northwest A&F University, Yangling, Shaanxi712100, China
关键词:
八仙花;  茎段; 组织培养
Keywords:
Hydrangea macrophylla(Thunb.)Seringe; stems with axillaries bud; tissue culture
分类号:
S723.132
文献标志码:
A
摘要:
以八仙花带腋芽茎段为外植体，研究了其组织培养技术。结果表明：以70%酒精处理30 s+0.1%升汞处理10 min，成活率可达96%；以MS+6BA0.5 mg/L+IBA0.5 mg/L为启动培养基，诱导率可达100%；以MS+6BA0.5 mg/L+IBA0.3 mg/L为增殖培养基，培养25 d，增殖系数可达9.2；以1/2MS+IBA0.3 mg/L 进行生根培养，生根率100%，根长势良好且根毛区长达1.1 cm；移栽到以珍珠岩∶腐殖土=1∶2的基质中，覆盖地膜保湿7 d，成活率可达80%以上。
Abstract:
Stems with axillaries bud of Hydrangea macrophylla (Thunb.)Seringe were used as explants to conduce in vitro culture. The results showed that the optimum disinfector was 70% alcohol for 30 seconds and HgCl2 for 10 minutes. The induction culture medium was MS+BA0.5 mg/L+IBA0.5 mg/L and the ratio of induction was 100%. The optimum multiplication culture medium was MS+BA0.5 mg/L+IBA 0.3 mg/L and after 25 days multiplication coefficient was 9.2. The rooting culture medium was 1/2MS+IBA 0.3mg/L and the ratio of rooting was 100%, and the root’s growth was better and the length of district root hairs was 1.1 cm. When transplanted in pots with the mediums of 1 perlite∶2 humus, most of the seedings could grow well.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：20080118修回日期：20080515 基金项目：西安市科技计划项目“秦巴山区重要野生观赏树木引种驯化与快速繁殖技术研究”（FY07107）。作者简介：雷亚灵，女，西北农林科技大学林木遗传育种专业硕士研究生。 *通讯作者：李周岐，男，西北农林科技大学林木遗传育种学教授，博士生导师。Emile:Lzhouqi@yahoo.com.cn
更新日期/Last Update: 2008-07-30